AGL66_ARATH
ID AGL66_ARATH Reviewed; 332 AA.
AC Q1PFC2; A0MEH0; Q76K85; Q7X9H4;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Agamous-like MADS-box protein AGL66 {ECO:0000305};
GN Name=AGL66 {ECO:0000303|PubMed:12837945};
GN OrderedLocusNames=At1g77980 {ECO:0000312|Araport:AT1G77980};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 26-332, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [6]
RP FUNCTION, INTERACTION WITH AGL30, AND DISRUPTION PHENOTYPE.
RX PubMed=19211705; DOI=10.1104/pp.109.135806;
RA Adamczyk B.J., Fernandez D.E.;
RT "MIKC* MADS domain heterodimers are required for pollen maturation and tube
RT growth in Arabidopsis.";
RL Plant Physiol. 149:1713-1723(2009).
CC -!- FUNCTION: Probable transcription factor that forms a heterodimer with
CC the MADS-box protein AGL30 and is involved in the regulation of pollen
CC maturation at the late stages of pollen development and pollen tube
CC growth. {ECO:0000269|PubMed:19211705}.
CC -!- SUBUNIT: Forms a heterodimer with AGL30. {ECO:0000269|PubMed:19211705}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- TISSUE SPECIFICITY: Expressed in pollen. {ECO:0000269|PubMed:12949148}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. Pollen grains from the double mutant agl66 and agl104 have
CC severely reduced viability, delayed germination and aberrant pollen
CC tube growth. {ECO:0000269|PubMed:19211705}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28473.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY141242; AAN52806.1; -; mRNA.
DR EMBL; AC009243; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE36053.1; -; Genomic_DNA.
DR EMBL; DQ446441; ABE65783.1; -; mRNA.
DR EMBL; DQ652940; ABK28473.1; ALT_SEQ; mRNA.
DR EMBL; AB094111; BAC99086.1; -; mRNA.
DR RefSeq; NP_177921.2; NM_106447.4.
DR AlphaFoldDB; Q1PFC2; -.
DR SMR; Q1PFC2; -.
DR IntAct; Q1PFC2; 6.
DR STRING; 3702.AT1G77980.1; -.
DR PaxDb; Q1PFC2; -.
DR PRIDE; Q1PFC2; -.
DR ProteomicsDB; 244943; -.
DR EnsemblPlants; AT1G77980.1; AT1G77980.1; AT1G77980.
DR GeneID; 844133; -.
DR Gramene; AT1G77980.1; AT1G77980.1; AT1G77980.
DR KEGG; ath:AT1G77980; -.
DR Araport; AT1G77980; -.
DR TAIR; locus:2029386; AT1G77980.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_048196_1_1_1; -.
DR InParanoid; Q1PFC2; -.
DR OMA; HSAMYET; -.
DR OrthoDB; 1160797at2759; -.
DR PhylomeDB; Q1PFC2; -.
DR PRO; PR:Q1PFC2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q1PFC2; baseline and differential.
DR Genevisible; Q1PFC2; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0010152; P:pollen maturation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..332
FT /note="Agamous-like MADS-box protein AGL66"
FT /id="PRO_0000433968"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT COILED 120..151
FT /evidence="ECO:0000255"
FT CONFLICT 60
FT /note="K -> E (in Ref. 1; AAN52806)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 332 AA; 38051 MW; 034CA58951E602E1 CRC64;
MGRVKLEIKR IENTTNRQVT FSKRRNGLIK KAYELSILCD IDIALLMFSP SDRLSLFSGK
TRIEDVFSRY INLSDQEREN ALVFPDQSRR PDFQSKEYLL RTLQQLKAEN DIALQLTNPT
AINSDVEELE HEVYKLQQQL LMAEEELRKY EPDPIRFTTM EEYETCEKQL MDTLTRVNQR
REHILSQDQL SSYEASALQQ QQSMGGPFGN DVVGGWLTEN GPNEAHLFDA SAHSAMYETL
LQGSSSSSNQ NNIMGESNVS NHNGDMFQEW AQAYNSTTAH NPSTLFPPMQ HQHGLVVDPN
IEEIEIPVMK KDAQADHEVS DYDIRMPQLS SQ
