AGL6_HALVD
ID AGL6_HALVD Reviewed; 413 AA.
AC D4GU74;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Low-salt glycan biosynthesis hexosyltransferase Agl6;
DE EC=2.4.1.-;
DE AltName: Full=Archaeal glycosylation protein 6;
GN Name=agl6; OrderedLocusNames=HVO_2061; ORFNames=C498_05593;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [3]
RP FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=24194539; DOI=10.1128/mbio.00716-13;
RA Kaminski L., Guan Z., Yurist-Doutsch S., Eichler J.;
RT "Two distinct N-glycosylation pathways process the Haloferax volcanii S-
RT layer glycoprotein upon changes in environmental salinity.";
RL MBio 4:E00716-E00716(2013).
CC -!- FUNCTION: Hexosyltransferase involved in N-glycan biosynthetic pathway
CC that takes place under low-salt conditions (1.75 M instead of 3.4 M).
CC Participates in the formation of the tetrasaccharide present at 'Asn-
CC 532' of S-layer glycoprotein Csg, consisting of a sulfated hexose, 2
CC hexoses and rhamnose. Together with Agl5, mediates the addition of
CC sugars 1 and 2 to dolichol phosphate in the tetrasaccharide.
CC {ECO:0000269|PubMed:24194539}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000269|PubMed:24194539}.
CC -!- PATHWAY: Cell surface structure biogenesis; S-layer biogenesis.
CC {ECO:0000269|PubMed:24194539}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Abolishes formation of the tetrasaccharide
CC present at 'Asn-532' of S-layer glycoprotein Csg. No effect on 'Asn-47'
CC and 'Asn-117' glycosylation of S-layer glycoprotein Csg.
CC {ECO:0000269|PubMed:24194539}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR EMBL; CP001956; ADE02267.1; -; Genomic_DNA.
DR EMBL; AOHU01000040; ELY33655.1; -; Genomic_DNA.
DR RefSeq; WP_004041946.1; NZ_AOHU01000040.1.
DR AlphaFoldDB; D4GU74; -.
DR STRING; 309800.C498_05593; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR EnsemblBacteria; ADE02267; ADE02267; HVO_2061.
DR EnsemblBacteria; ELY33655; ELY33655; C498_05593.
DR GeneID; 8926118; -.
DR KEGG; hvo:HVO_2061; -.
DR PATRIC; fig|309800.29.peg.1085; -.
DR eggNOG; arCOG00894; Archaea.
DR eggNOG; arCOG01910; Archaea.
DR HOGENOM; CLU_033536_4_0_2; -.
DR OMA; EYFPQMC; -.
DR OrthoDB; 96698at2157; -.
DR UniPathway; UPA00378; -.
DR UniPathway; UPA00977; -.
DR Proteomes; UP000008243; Chromosome.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR GO; GO:0045232; P:S-layer organization; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Membrane; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..413
FT /note="Low-salt glycan biosynthesis hexosyltransferase
FT Agl6"
FT /id="PRO_0000428770"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 413 AA; 43811 MW; DBE574DFBDEDD406 CRC64;
MSTRSQSESP VDAPQQGATN GQSASDIHHS GDELLLSADS DIAPTLSVVM PTLNEEGGIA
QCIEWVKAAL EDMQIYGEVV VADSSTDRTP EIAAENGAIV IEPDGKGYGY AYLYAFERVR
GDYIAMGDAD CTYDFEELPK LLNMVRSGDA DMAMGSRLEG EILPGSMPPL HEHVGNPLLT
KFLNVFYGAG VSDAHSGMRV FSRDAWETMD CSSTGMEFAS EMIMEAGAKD LEIKEKPITY
HPREGEANLE SFPDGWRHVR FMLVNAPGYL FSAPGFGLSV IGVLALVLAW SGVEVGGAQF
GIHTGIGGGL LTLAGFQLML FGAFSTVSSD PVRGASDPFT TWFTERISLE RGATIGSVVL
LCGLAYGGLL AFTWVTSGFS ALPIAVADVV ATVAVVIGLQ MVFGSFLLGS LGE
