AGL70_ARATH
ID AGL70_ARATH Reviewed; 196 AA.
AC Q9LSR7;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=Agamous-like MADS-box protein AGL70 {ECO:0000303|PubMed:22295091};
DE AltName: Full=MADS box FLC1-like nuclear protein 3 {ECO:0000303|PubMed:22295091};
DE AltName: Full=Protein AGAMOUS-like 70 {ECO:0000303|PubMed:22295091};
DE AltName: Full=Protein MADS AFFECTING FLOWERING 3 {ECO:0000303|PubMed:12724541};
GN Name=AGL70 {ECO:0000303|PubMed:22295091};
GN Synonyms=FCL3 {ECO:0000303|PubMed:22295091},
GN MAF3 {ECO:0000303|PubMed:12724541};
GN OrderedLocusNames=At5g65060 {ECO:0000312|Araport:AT5G65060};
GN ORFNames=F15O5.2 {ECO:0000312|EMBL:BAA97510.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND REPRESSION BY VERNALIZATION.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=12724541; DOI=10.1105/tpc.009506;
RA Ratcliffe O.J., Kumimoto R.W., Wong B.J., Riechmann J.L.;
RT "Analysis of the Arabidopsis MADS AFFECTING FLOWERING gene family: MAF2
RT prevents vernalization by short periods of cold.";
RL Plant Cell 15:1159-1169(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Hau-0, cv. Li-8, and cv. Nd-0;
RX PubMed=19139056; DOI=10.1093/molbev/msn300;
RA Caicedo A.L., Richards C., Ehrenreich I.M., Purugganan M.D.;
RT "Complex rearrangements lead to novel chimeric gene fusion polymorphisms at
RT the Arabidopsis thaliana MAF2-5 flowering time gene cluster.";
RL Mol. Biol. Evol. 26:699-711(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=12949148; DOI=10.1093/molbev/msg216;
RA Kofuji R., Sumikawa N., Yamasaki M., Kondo K., Ueda K., Ito M., Hasebe M.;
RT "Evolution and divergence of the MADS-box gene family based on genome-wide
RT expression analyses.";
RL Mol. Biol. Evol. 20:1963-1977(2003).
RN [6]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=16547097; DOI=10.1534/genetics.106.056580;
RA Wang J., Tian L., Lee H.S., Chen Z.J.;
RT "Nonadditive regulation of FRI and FLC loci mediates flowering-time
RT variation in Arabidopsis allopolyploids.";
RL Genetics 173:965-974(2006).
RN [8]
RP GENE FAMILY.
RX PubMed=22295091; DOI=10.1371/journal.pone.0030524;
RA Severing E.I., van Dijk A.D.J., Morabito G., Busscher-Lange J.,
RA Immink R.G.H., van Ham R.C.H.J.;
RT "Predicting the impact of alternative splicing on plant MADS domain protein
RT function.";
RL PLoS ONE 7:E30524-E30524(2012).
RN [9]
RP REGULATION BY HAM1 AND HAM2.
RC STRAIN=cv. C24, and cv. Columbia;
RX PubMed=23273925; DOI=10.1016/j.jplph.2012.11.007;
RA Xiao J., Zhang H., Xing L., Xu S., Liu H., Chong K., Xu Y.;
RT "Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic
RT modification of FLC in regulating flowering in Arabidopsis.";
RL J. Plant Physiol. 170:444-451(2013).
RN [10]
RP FUNCTION.
RC STRAIN=cv. Col-FRI, and cv. Columbia;
RX PubMed=25339407; DOI=10.1104/pp.114.247346;
RA Suter L., Rueegg M., Zemp N., Hennig L., Widmer A.;
RT "Gene regulatory variation mediates flowering responses to vernalization
RT along an altitudinal gradient in Arabidopsis.";
RL Plant Physiol. 166:1928-1942(2014).
CC -!- FUNCTION: Probable transcription factor involved in the negative
CC regulation of flowering time, probably through the photoperiodic and
CC vernalization pathways; more efficient in cv. Landsberg erecta than in
CC cv. Columbia background. Prevents premature flowering (PubMed:12724541,
CC PubMed:25339407). Involved in the modulation of vernalization impact on
CC flowering according to genotype acclimation to altitude
CC (PubMed:25339407). {ECO:0000269|PubMed:12724541,
CC ECO:0000269|PubMed:25339407}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251,
CC ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots, leaves and flowers, and,
CC to a lower extent, in inflorescence, siliques, pollen and shoots.
CC {ECO:0000269|PubMed:12837945, ECO:0000269|PubMed:12949148}.
CC -!- INDUCTION: Repressed during vernalization (PubMed:12724541). Regulated
CC by HAM1 and HAM2 via epigenetic modification of chromatins at H4K5
CC acetylation during flowering (PubMed:23273925).
CC {ECO:0000269|PubMed:12724541, ECO:0000269|PubMed:23273925}.
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DR EMBL; AY231445; AAO65310.1; -; mRNA.
DR EMBL; EU980617; ACL93409.1; -; Genomic_DNA.
DR EMBL; EU980619; ACL93416.1; -; Genomic_DNA.
DR EMBL; EU980621; ACL93423.1; -; Genomic_DNA.
DR EMBL; EU980626; ACL93443.1; -; Genomic_DNA.
DR EMBL; AB026633; BAA97510.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97993.1; -; Genomic_DNA.
DR RefSeq; NP_201311.1; NM_125905.4.
DR AlphaFoldDB; Q9LSR7; -.
DR SMR; Q9LSR7; -.
DR STRING; 3702.AT5G65060.1; -.
DR PaxDb; Q9LSR7; -.
DR EnsemblPlants; AT5G65060.1; AT5G65060.1; AT5G65060.
DR GeneID; 836630; -.
DR Gramene; AT5G65060.1; AT5G65060.1; AT5G65060.
DR KEGG; ath:AT5G65060; -.
DR Araport; AT5G65060; -.
DR TAIR; locus:2144345; AT5G65060.
DR eggNOG; KOG0014; Eukaryota.
DR InParanoid; Q9LSR7; -.
DR OrthoDB; 1433397at2759; -.
DR PhylomeDB; Q9LSR7; -.
DR PRO; PR:Q9LSR7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LSR7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010582; P:floral meristem determinacy; IBA:GO_Central.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:TAIR.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010048; P:vernalization response; IDA:UniProtKB.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..196
FT /note="Agamous-like MADS-box protein AGL70"
FT /id="PRO_0000441234"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 80..170
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT MOTIF 8..15
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
SQ SEQUENCE 196 AA; 22129 MW; E147706CD3DF6A46 CRC64;
MGRRKVEIKR IENKSSRQVT FSKRRKGLIE KARQLSILCE SSIAVVAVSG SGKLYDSASG
DNMSKIIDRY EIHHADELKA LDLAEKIRNY LPHKELLEIV QSKLEESNVD NVSVDSLISM
EEQLETALSV IRAKKTELMM EDMKSLQERE KLLIEENQIL ASQVGKKTFL VIEGDRGMSR
ENGSGNKVPE TLSLLK
