AGL8_ARATH
ID AGL8_ARATH Reviewed; 242 AA.
AC Q38876;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Agamous-like MADS-box protein AGL8;
DE AltName: Full=Floral homeotic protein AGL8;
DE AltName: Full=Transcription factor FRUITFULL;
GN Name=AGL8; Synonyms=FUL; OrderedLocusNames=At5g60910; ORFNames=MSL3.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8535133; DOI=10.2307/3870185;
RA Mandel M.A., Yanofsky M.F.;
RT "The Arabidopsis AGL8 MADS box gene is expressed in inflorescence meristems
RT and is negatively regulated by APETALA1.";
RL Plant Cell 7:1763-1771(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=9502732; DOI=10.1242/dev.125.8.1509;
RA Gu Q., Ferrandiz C., Yanofsky M.F., Martienssen R.;
RT "The FRUITFULL MADS-box gene mediates cell differentiation during
RT Arabidopsis fruit development.";
RL Development 125:1509-1517(1998).
RN [6]
RP FUNCTION.
RX PubMed=10648231; DOI=10.1242/dev.127.4.725;
RA Ferrandiz C., Gu Q., Martienssen R., Yanofsky M.F.;
RT "Redundant regulation of meristem identity and plant architecture by
RT FRUITFULL, APETALA1 and CAULIFLOWER.";
RL Development 127:725-734(2000).
RN [7]
RP FUNCTION.
RX PubMed=15035986; DOI=10.1016/s0092-8674(04)00217-x;
RA Liljegren S.J., Roeder A.H.K., Kempin S.A., Gremski K., Ostergaard L.,
RA Guimil S., Reyes D.K., Yanofsky M.F.;
RT "Control of fruit patterning in Arabidopsis by INDEHISCENT.";
RL Cell 116:843-853(2004).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=28586421; DOI=10.1093/jxb/erx184;
RA Bemer M., van Mourik H., Muino J.M., Ferrandiz C., Kaufmann K.,
RA Angenent G.C.;
RT "FRUITFULL controls SAUR10 expression and regulates Arabidopsis growth and
RT architecture.";
RL J. Exp. Bot. 68:3391-3403(2017).
CC -!- FUNCTION: Probable transcription factor that promotes early floral
CC meristem identity in synergy with APETALA1 and CAULIFLOWER. Is required
CC subsequently for the transition of an inflorescence meristem into a
CC floral meristem (PubMed:28586421). Seems to be partially redundant to
CC the function of APETALA1 and CAULIFLOWER in the up-regulation of LEAFY.
CC Is also required for normal pattern of cell division, expansion and
CC differentiation during morphogenesis of the silique (PubMed:28586421).
CC Probably not required for fruit elongation but instead is required to
CC prevent ectopic activity of IND. Represses SAUR10 expression in stems
CC and inflorescence branches (PubMed:28586421).
CC {ECO:0000269|PubMed:10648231, ECO:0000269|PubMed:15035986,
CC ECO:0000269|PubMed:28586421, ECO:0000269|PubMed:9502732}.
CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q38876; O82794: AGL24; NbExp=3; IntAct=EBI-621912, EBI-592083;
CC Q38876; O64645: SOC1; NbExp=4; IntAct=EBI-621912, EBI-592041;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q38876-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Vascular tissue of cauline leaves, floral shoot
CC apex and valves of carpels and fruits. {ECO:0000269|PubMed:9502732}.
CC -!- INDUCTION: Dramatically up-regulated upon the transition from
CC vegetative to reproductive development.
CC -!- DISRUPTION PHENOTYPE: Altered cauline leaf shape, abnormal stem
CC development and architecture, including enhanced branching, decreased
CC inflorescence branch angles, and shorter stem and internode lengths.
CC Reduced stems elongation after bolting. {ECO:0000269|PubMed:28586421}.
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DR EMBL; U33473; AAA97403.1; -; mRNA.
DR EMBL; AB008269; BAB10640.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97395.1; -; Genomic_DNA.
DR EMBL; AF386929; AAK62374.1; -; mRNA.
DR EMBL; AY072463; AAL66878.1; -; mRNA.
DR PIR; S71208; S71208.
DR RefSeq; NP_568929.1; NM_125484.4. [Q38876-1]
DR AlphaFoldDB; Q38876; -.
DR SMR; Q38876; -.
DR BioGRID; 21456; 17.
DR DIP; DIP-33794N; -.
DR IntAct; Q38876; 19.
DR STRING; 3702.AT5G60910.1; -.
DR PaxDb; Q38876; -.
DR PRIDE; Q38876; -.
DR ProteomicsDB; 244732; -. [Q38876-1]
DR EnsemblPlants; AT5G60910.1; AT5G60910.1; AT5G60910. [Q38876-1]
DR GeneID; 836212; -.
DR Gramene; AT5G60910.1; AT5G60910.1; AT5G60910. [Q38876-1]
DR KEGG; ath:AT5G60910; -.
DR Araport; AT5G60910; -.
DR TAIR; locus:2173522; AT5G60910.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR InParanoid; Q38876; -.
DR OMA; ISQIENW; -.
DR PhylomeDB; Q38876; -.
DR PRO; PR:Q38876; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q38876; baseline and differential.
DR Genevisible; Q38876; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0060560; P:developmental growth involved in morphogenesis; IDA:UniProtKB.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0010154; P:fruit development; IMP:TAIR.
DR GO; GO:0010077; P:maintenance of inflorescence meristem identity; IGI:TAIR.
DR GO; GO:0009911; P:positive regulation of flower development; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Coiled coil; Developmental protein;
KW Differentiation; DNA-binding; Flowering; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..242
FT /note="Agamous-like MADS-box protein AGL8"
FT /id="PRO_0000199461"
FT DOMAIN 3..57
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 88..178
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT COILED 89..178
FT /evidence="ECO:0000255"
SQ SEQUENCE 242 AA; 27536 MW; 516499731EADB2D2 CRC64;
MGRGRVQLKR IENKINRQVT FSKRRSGLLK KAHEISVLCD AEVALIVFSS KGKLFEYSTD
SCMERILERY DRYLYSDKQL VGRDVSQSEN WVLEHAKLKA RVEVLEKNKR NFMGEDLDSL
SLKELQSLEH QLDAAIKSIR SRKNQAMFES ISALQKKDKA LQDHNNSLLK KIKEREKKTG
QQEGQLVQCS NSSSVLLPQY CVTSSRDGFV ERVGGENGGA SSLTEPNSLL PAWMLRPTTT
NE