ID   AGL9_HALVD              Reviewed;         359 AA.
AC   D4GU62;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Low-salt glycan biosynthesis hexosyltransferase Agl9;
DE            EC=2.4.1.-;
DE   AltName: Full=Archaeal glycosylation protein 9;
GN   Name=agl9; OrderedLocusNames=HVO_2048; ORFNames=C498_05531;
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
RN   [3]
RP   FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=24194539; DOI=10.1128/mbio.00716-13;
RA   Kaminski L., Guan Z., Yurist-Doutsch S., Eichler J.;
RT   "Two distinct N-glycosylation pathways process the Haloferax volcanii S-
RT   layer glycoprotein upon changes in environmental salinity.";
RL   MBio 4:E00716-E00716(2013).
CC   -!- FUNCTION: Hexosyltransferase involved in N-glycan biosynthetic pathway
CC       that takes place under low-salt conditions (1.75 M instead of 3.4 M).
CC       Participates in the formation of the tetrasaccharide present at 'Asn-
CC       532' of S-layer glycoprotein Csg, consisting of a sulfated hexose, 2
CC       hexoses and rhamnose. Involved in the addition of sugar 3 to the
CC       disaccharide-charged dolichol phosphate in the tetrasaccharide.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- PATHWAY: Cell surface structure biogenesis; S-layer biogenesis.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- DISRUPTION PHENOTYPE: Impaired formation of the tetrasaccharide present
CC       at 'Asn-532' of S-layer glycoprotein Csg. No effect on 'Asn-47' and
CC       'Asn-117' glycosylation of S-layer glycoprotein Csg.
CC       {ECO:0000269|PubMed:24194539}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       {ECO:0000305}.
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DR   EMBL; CP001956; ADE05081.1; -; Genomic_DNA.
DR   EMBL; AOHU01000038; ELY34564.1; -; Genomic_DNA.
DR   RefSeq; WP_004041934.1; NZ_AOHU01000038.1.
DR   AlphaFoldDB; D4GU62; -.
DR   SMR; D4GU62; -.
DR   STRING; 309800.C498_05531; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   EnsemblBacteria; ADE05081; ADE05081; HVO_2048.
DR   EnsemblBacteria; ELY34564; ELY34564; C498_05531.
DR   GeneID; 8925606; -.
DR   KEGG; hvo:HVO_2048; -.
DR   eggNOG; arCOG01403; Archaea.
DR   HOGENOM; CLU_009583_2_2_2; -.
DR   OMA; DLISTHY; -.
DR   OrthoDB; 83551at2157; -.
DR   UniPathway; UPA00378; -.
DR   UniPathway; UPA00977; -.
DR   Proteomes; UP000008243; Chromosome.
DR   Proteomes; UP000011532; Unassembled WGS sequence.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR   GO; GO:0045232; P:S-layer organization; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13439; Glyco_transf_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..359
FT                   /note="Low-salt glycan biosynthesis hexosyltransferase
FT                   Agl9"
FT                   /id="PRO_0000428769"
SQ   SEQUENCE   359 AA;  40535 MW;  CF144B2C25418B24 CRC64;
     MSSDYSNISE LIEEEYNNSK SVGIFVSRFD KSEGVGTVVN RHISELNDAG IDVTLFAYEQ
     VDEIAEKKVV NVSSSLTGNN TLNKLISSMN PLKWLSLSSE LKKLDLLIVH QPILCSIAYY
     AQKIHDIDTI YYNHEVTRPE DKVGTIQRIY GYTLFQIYLH ISSRLDQIVS VSKYSQQEYS
     RRFDRQGMVI HNSIDHEMYN QSVDGSRIRE KYDLEDDPLV LFVGRIAKSK GIHRLISVFE
     DVSKTIPDAT LVIVGRPDNQ GYYQRVKVLS KSVDQQIIFA GIVPEEDLPA YYSAADLYAT
     CSIKEGYNLT IAEAEACGTP TIAFDIGAHS EVMNDGVLVP KDNTSLFKEE MVSQLTKQR