ID   ENO_IMPCY               Reviewed;          32 AA.
AC   P84208;
DT   09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   09-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Enolase;
DE            EC=4.2.1.11;
DE   AltName: Full=2-phospho-D-glycerate hydro-lyase;
DE   AltName: Full=2-phosphoglycerate dehydratase;
DE   Flags: Fragments;
OS   Imperata cylindrica (Cogon grass).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Germainiinae; Imperata.
OX   NCBI_TaxID=80369;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. Major {ECO:0000269|Ref.1}; TISSUE=Leaf {ECO:0000269|Ref.1};
RA   Chang I.-F.;
RT   "Ecotypic variation of a medicinal plant Imperata cylindrica populations in
RT   Taiwan: mass spectrometry-based proteomic evidence.";
RL   J. Med. Plant Res. 2940:71-76(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate;
CC         Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289,
CC         ChEBI:CHEBI:58702; EC=4.2.1.11;
CC         Evidence={ECO:0000250|UniProtKB:P00924};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Mg(2+) is required for catalysis and for stabilizing the dimer.
CC       {ECO:0000250};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 4/5.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P00924}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the enolase family. {ECO:0000255}.
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DR   AlphaFoldDB; P84208; -.
DR   SMR; P84208; -.
DR   UniPathway; UPA00109; UER00187.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004634; F:phosphopyruvate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036849; Enolase-like_C_sf.
DR   SUPFAM; SSF51604; SSF51604; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium.
FT   CHAIN           <1..>32
FT                   /note="Enolase"
FT                   /id="PRO_0000134071"
FT   NON_CONS        17..18
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|Ref.1"
FT   NON_TER         32
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   32 AA;  3292 MW;  C7300FE8679AB33E CRC64;
     SGETEDVTIA DIVVGLRIEE ELGDAAVYAG AK