AGLU_MUCJA
ID AGLU_MUCJA Reviewed; 864 AA.
AC Q92442;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Alpha-glucosidase;
DE EC=3.2.1.20;
DE AltName: Full=Maltase;
DE Flags: Precursor;
OS Mucor javanicus.
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC Mucoromycetes; Mucorales; Mucorineae; Mucoraceae; Mucor.
OX NCBI_TaxID=51122;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=DSM 1222 / BCRC 31544 / NBRC 4570 / NRRL 13035;
RX PubMed=8830045; DOI=10.1093/oxfordjournals.jbchem.a021269;
RA Sugimoto M., Suzuki Y.;
RT "Molecular cloning, sequencing, and expression of a cDNA encoding alpha-
RT glucosidase from Mucor javanicus.";
RL J. Biochem. 119:500-505(1996).
CC -!- FUNCTION: Hydrolyzes not only malto-oligosaccharides but also soluble
CC starch.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-
CC glucose residues with release of alpha-D-glucose.; EC=3.2.1.20;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family. {ECO:0000305}.
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DR EMBL; D67034; BAA11053.1; -; mRNA.
DR AlphaFoldDB; Q92442; -.
DR SMR; Q92442; -.
DR CAZy; GH31; Glycoside Hydrolase Family 31.
DR CLAE; AGL31A_MUCJA; -.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0032450; F:maltose alpha-glucosidase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.60.40.1180; -; 2.
DR InterPro; IPR031727; Gal_mutarotase_N.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR000322; Glyco_hydro_31.
DR InterPro; IPR030458; Glyco_hydro_31_AS.
DR InterPro; IPR030459; Glyco_hydro_31_CS.
DR InterPro; IPR025887; Glyco_hydro_31_N_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF13802; Gal_mutarotas_2; 1.
DR Pfam; PF01055; Glyco_hydro_31; 1.
DR Pfam; PF16863; NtCtMGAM_N; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF74650; SSF74650; 1.
DR PROSITE; PS00129; GLYCOSYL_HYDROL_F31_1; 1.
DR PROSITE; PS00707; GLYCOSYL_HYDROL_F31_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..864
FT /note="Alpha-glucosidase"
FT /id="PRO_0000018580"
FT ACT_SITE 430
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10066"
FT ACT_SITE 433
FT /evidence="ECO:0000250"
FT ACT_SITE 567
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 364
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 406
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 466
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 500
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 568
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 734
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 864 AA; 98761 MW; CFAB4759DC431403 CRC64;
MAKVSFIFVA IALITGNVLC QTDATYAVSS SAPGYKIDGH VRKTEAGLHI PLTLNSRGNK
KTGIDTFGKT IKDITVDVEY ETEERLHVKI SDKAKKQYLV PDSPLGFERP QIKHYVSPKH
SNLDFQYTAK PFSFKVVRKD DKTTIFDTTN MPLVFEDQYL ELSTKVPEDA NIYGIGEVTA
PFRRTHNVTT LWARDNPDDF YRNIYGAHPY YQEVRDGKAH GALLMNAHGM DVITTEGRIT
YKVIGGILDF YFFAPKSGKP NDLSIAYTDL IGKPMMPSHW MLGWHHCRYG YPNIDKVETV
KRKYKEANIP LQTVWVDIDY MEETKDFTFD KVNFPQDRMI GLGEQLHKDG QNYVVMVDPA
ISANTTYEPY VRGTEMDVWI KNADGSDFIG SVWPGFTTFP DWWHPNATKY WNKEIIDFVD
MLGVDGLWID MNEPASFCLG SCGSGKVDAG NQPYRWTYTE EEQAANHTRW EKELKAMGNP
PGEERNLLYP KYAINNGAGN LSEFTVATTA LHYGNIPHYD IHNLYGHAES HITRQALIKH
KNKIRPFVLT RSSFPGSGKS VGHWTGDNHS FWPYLKNSIA NILNFQMFGV SYSGADVCGF
NSDTTEELCT RWMEIGAFYP FARNHNNNAA KDQEPYLWES TAEASRIAIN TRYEMLPYFY
TLFEESNRLG LGVWRPLIFE YPAYEELVSN DVQTLVGSDI LLSPVLDEGK TSVKAQFPGG
QWYDWYTHEL TVDNKSNKKV KTVTLDAPLT HIPIHIRGGA IIPTKTPKYT VGETFATPYN
LVIALDKKGQ ASGRLYIDDG ESLEVKSSSG YHFHLQEWSP QGFWQVWLQE GRKDWLHHHY
WQARQQVAKG SRWQEDYQID PWQE