AGLU_ORYSJ
ID AGLU_ORYSJ Reviewed; 885 AA.
AC Q653V7; Q0DA62;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Probable alpha-glucosidase Os06g0675700;
DE EC=3.2.1.20;
DE AltName: Full=Maltase;
DE Flags: Precursor;
GN OrderedLocusNames=Os06g0675700, LOC_Os06g46284;
GN ORFNames=B1153E06.2, OsJ_22347 {ECO:0000312|EMBL:EEE66212.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP PROTEIN SEQUENCE OF 34-43.
RX PubMed=10617595; DOI=10.1074/jbc.275.1.129;
RA Chang T., Kuo M.-C., Khoo K.-H., Inoue S., Inoue Y.;
RT "Developmentally regulated expression of a peptide:N-glycanase during
RT germination of rice seeds (Oryza sativa) and its purification and
RT characterization.";
RL J. Biol. Chem. 275:129-134(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-
CC glucose residues with release of alpha-D-glucose.; EC=3.2.1.20;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family. {ECO:0000305}.
CC -!- CAUTION: Was isolated and reported to have peptide:N-glycanase activity
CC (PubMed:10617595). However, its strong sequence similarity with alpha-
CC glucosidase proteins suggest that it is not its function in vivo.
CC {ECO:0000305|PubMed:10617595}.
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DR EMBL; AP004989; BAD45910.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF20261.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS99103.1; -; Genomic_DNA.
DR EMBL; CM000143; EEE66212.1; -; Genomic_DNA.
DR EMBL; AK119824; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015643120.1; XM_015787634.1.
DR AlphaFoldDB; Q653V7; -.
DR SMR; Q653V7; -.
DR STRING; 4530.OS06T0675700-01; -.
DR BindingDB; Q653V7; -.
DR ChEMBL; CHEMBL1163102; -.
DR CAZy; GH31; Glycoside Hydrolase Family 31.
DR PaxDb; Q653V7; -.
DR PRIDE; Q653V7; -.
DR EnsemblPlants; Os06t0675700-01; Os06t0675700-01; Os06g0675700.
DR GeneID; 4341824; -.
DR Gramene; Os06t0675700-01; Os06t0675700-01; Os06g0675700.
DR KEGG; osa:4341824; -.
DR eggNOG; KOG1065; Eukaryota.
DR HOGENOM; CLU_000631_11_1_1; -.
DR InParanoid; Q653V7; -.
DR OMA; FGVETCG; -.
DR OrthoDB; 151244at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IBA:GO_Central.
DR GO; GO:0032450; F:maltose alpha-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0044275; P:cellular carbohydrate catabolic process; IEA:UniProt.
DR Gene3D; 2.60.40.1180; -; 2.
DR InterPro; IPR031727; Gal_mutarotase_N.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR000322; Glyco_hydro_31.
DR InterPro; IPR030458; Glyco_hydro_31_AS.
DR InterPro; IPR030459; Glyco_hydro_31_CS.
DR InterPro; IPR025887; Glyco_hydro_31_N_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR Pfam; PF13802; Gal_mutarotas_2; 1.
DR Pfam; PF01055; Glyco_hydro_31; 1.
DR Pfam; PF16863; NtCtMGAM_N; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF74650; SSF74650; 1.
DR PROSITE; PS00129; GLYCOSYL_HYDROL_F31_1; 1.
DR PROSITE; PS00707; GLYCOSYL_HYDROL_F31_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000269|PubMed:10617595"
FT CHAIN 34..885
FT /note="Probable alpha-glucosidase Os06g0675700"
FT /id="PRO_0000249005"
FT ACT_SITE 443
FT /evidence="ECO:0000250"
FT ACT_SITE 446
FT /evidence="ECO:0000250"
FT ACT_SITE 540
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 378
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 397
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 467
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 477
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 576
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 844
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 34
FT /note="G -> S (in Ref. 6; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 43
FT /note="S -> L (in Ref. 6; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="A -> P (in Ref. 5; AK119824)"
FT /evidence="ECO:0000305"
FT CONFLICT 445
FT /note="N -> S (in Ref. 5; AK119824)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 885 AA; 96336 MW; 34ABDF8A194C75A5 CRC64;
MMGSPPAPPA RRLGALAVFL LALFLAAPWG VDCGYNVASV AGSKNRLRAR LELAGGGGGA
APELGPDVRR LSLTASLETD SRLHVRITDA DHPRWEVPQD VIPRPSPDSF LAATRPGGGR
VLSTATSDLT FAIHTSPFRF TVTRRSTGDV LFDTTPNLVF KDRYLELTSS LPPPGRASLY
GLGEQTKRTF RLQRNDTFTL WNSDIAAGNV DLNLYGSHPF YMDVRSGGGG GGGAAHGVLL
LNSNGMDVIY GGSYVTYKVI GGVLDFYFFA GPSPLAVVDQ YTQLIGRPAP MPYWSFGFHQ
CRYGYKNVAD LEGVVAGYAK ARIPLEVMWT DIDYMDAYKD FTLDPVNFPA DRMRPFVDRL
HRNGQKFVVI IDPGINVNTT YGTFVRGMKQ DIFLKWNGSN YLGVVWPGNV YFPDFLNPRA
AEFWAREIAA FRRTLPVDGL WVDMNEISNF VDPPPLNAID DPPYRINNSG VRRPINNKTV
PASAVHYGGV AEYDAHNLFG FLEARATHDA LLRDTGRRPF VLSRSTFVGS GRYTAHWTGD
NAATWEDLHY SINTMLSFGL FGIPMIGADI CGFGGNTTEE LCSRWIQLGA FYPFSRDHSA
IGTVRRELYL WESVARSARK ALGLRYRLLP YLYTLMYEAH TTGAPIARPL FFSYPGDVET
YGIDRQFLLG RGVLVSPVLE PGATTVTAYF PAGRWFSLYD FSLAVATKTG KRVTLPAPAD
TVNVHVAGGN ILTLQQPALT SSRVRQSVVH LLVALADDGT ATGDLFLDDG ESPEMAGPRS
RWSQIKFSGA TESGGGVVRV RSHVVHDSYA PSRTMAIGKV VLMGLRSAAP PKGFAVYANG
VQVNASTAVG GAAGSPEKGA LGVAHVSGLT LVVGQEFDLK VVMTY