3MGA_HAEIN
ID 3MGA_HAEIN Reviewed; 185 AA.
AC P44321;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=DNA-3-methyladenine glycosylase;
DE EC=3.2.2.20;
DE AltName: Full=3-methyladenine-DNA glycosidase;
DE Short=TAG;
GN Name=tag; OrderedLocusNames=HI_0654;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-
CC methyladenine from the damaged DNA polymer formed by alkylation
CC lesions. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of alkylated DNA, releasing 3-methyladenine.;
CC EC=3.2.2.20;
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DR EMBL; L42023; AAC22313.1; -; Genomic_DNA.
DR PIR; G64084; G64084.
DR RefSeq; NP_438814.1; NC_000907.1.
DR RefSeq; WP_005694489.1; NC_000907.1.
DR AlphaFoldDB; P44321; -.
DR SMR; P44321; -.
DR STRING; 71421.HI_0654; -.
DR EnsemblBacteria; AAC22313; AAC22313; HI_0654.
DR KEGG; hin:HI_0654; -.
DR PATRIC; fig|71421.8.peg.683; -.
DR eggNOG; COG2818; Bacteria.
DR HOGENOM; CLU_083758_1_0_6; -.
DR OMA; YVAYHDT; -.
DR PhylomeDB; P44321; -.
DR BioCyc; HINF71421:G1GJ1-689-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0008725; F:DNA-3-methyladenine glycosylase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006284; P:base-excision repair; IEA:InterPro.
DR GO; GO:0006307; P:DNA dealkylation involved in DNA repair; IBA:GO_Central.
DR InterPro; IPR005019; Adenine_glyco.
DR InterPro; IPR011257; DNA_glycosylase.
DR InterPro; IPR004597; Tag.
DR Pfam; PF03352; Adenine_glyco; 1.
DR SUPFAM; SSF48150; SSF48150; 1.
DR TIGRFAMs; TIGR00624; tag; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; Hydrolase; Metal-binding; Reference proteome; Zinc.
FT CHAIN 1..185
FT /note="DNA-3-methyladenine glycosylase"
FT /id="PRO_0000194879"
FT BINDING 5
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 19
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 176
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 185 AA; 21193 MW; 03AC41B636BD4321 CRC64;
MTTRCPWVGE QSIYIDYHDK EWGKPEFDSQ KLFEKICLEG QQAGLSWITV LKKRESYREA
FHQFDPKKIA KMTALDIDAC MQNSGLIRHR AKLEAIVKNA KAYLAMEKCG ENFSDFIWSF
VNHKPIVNDV PDLRSVPTKT EVSKALSKAL KKRGFVFIGE TTCYAFMQSM GLVDDHLNDC
PCKTS