ENT1_ARATH
ID ENT1_ARATH Reviewed; 450 AA.
AC Q8VXY7; O64603; Q9M5X9;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Equilibrative nucleotide transporter 1;
DE Short=AtENT1;
DE AltName: Full=Nucleoside transporter ENT1;
GN Name=ENT1; OrderedLocusNames=At1g70330; ORFNames=F17O7.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-450.
RA Li J., Wang D.;
RT "Cloning and in vitro expression of the cDNA of a putative nucleoside
RT transporter from Arabidopsis thaliana.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=11749958; DOI=10.1016/s0014-5793(01)03195-7;
RA Moehlmann T., Mezher Z., Schwerdtfeger G., Neuhaus H.E.;
RT "Characterisation of a concentrative type of adenosine transporter from
RT Arabidopsis thaliana (ENT1,At).";
RL FEBS Lett. 509:370-374(2001).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=12810710; DOI=10.1074/jbc.m304768200;
RA Li G., Liu K., Baldwin S.A., Wang D.;
RT "Equilibrative nucleoside transporters of Arabidopsis thaliana. cDNA
RT cloning, expression pattern, and analysis of transport activities.";
RL J. Biol. Chem. 278:35732-35742(2003).
RN [7]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=15228386; DOI=10.1042/bj20040389;
RA Wormit A., Traub M., Floerchinger M., Neuhaus H.E., Moehlmann T.;
RT "Characterization of three novel members of the Arabidopsis thaliana
RT equilibrative nucleoside transporter (ENT) family.";
RL Biochem. J. 383:19-26(2004).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=17151019; DOI=10.1074/mcp.m600250-mcp200;
RA Jaquinod M., Villiers F., Kieffer-Jaquinod S., Hugouvieux V., Bruley C.,
RA Garin J., Bourguignon J.;
RT "A proteomics dissection of Arabidopsis thaliana vacuoles isolated from
RT cell culture.";
RL Mol. Cell. Proteomics 6:394-412(2007).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21642237; DOI=10.1093/jxb/err183;
RA Bernard C., Traub M., Kunz H.H., Hach S., Trentmann O., Moehlmann T.;
RT "Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen
RT germination and vegetative growth in Arabidopsis.";
RL J. Exp. Bot. 62:4627-4637(2011).
CC -!- FUNCTION: Nucleoside transporter involved in adenosine transport and
CC required for nucleotide metabolism which influences growth and pollen
CC germination. Has high affinity for adenosine when expressed in a
CC heterologous system (yeast). {ECO:0000269|PubMed:11749958,
CC ECO:0000269|PubMed:15228386, ECO:0000269|PubMed:21642237}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=3.6 uM for adenosine {ECO:0000269|PubMed:11749958,
CC ECO:0000269|PubMed:15228386};
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:17151019,
CC ECO:0000305|PubMed:21642237}; Multi-pass membrane protein {ECO:0000255,
CC ECO:0000305|PubMed:21642237}. Note=Tonoplast.
CC -!- TISSUE SPECIFICITY: In young seedlings, expressed in root elongation
CC zone, root cortex, root-hair, at the transition to the shoot and
CC cotyledons. Expressed in hydathodes of fully developed leaves and
CC pollen. {ECO:0000269|PubMed:12810710}.
CC -!- INDUCTION: By nitrogen deficiency and 5-fluorouracil plus methotrexate.
CC {ECO:0000269|PubMed:12810710}.
CC -!- MISCELLANEOUS: Plants over-expressing ENT1 have decreased leaf content
CC of adenosine and show growth deficiencies. Plants silencing ENT1 have
CC increased leaf content of adenosine and decreased rate of pollen
CC germination. {ECO:0000305|PubMed:21642237}.
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC18807.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC003671; AAC18807.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE35046.1; -; Genomic_DNA.
DR EMBL; AY074345; AAL67041.1; -; mRNA.
DR EMBL; BT003008; AAO22816.1; -; mRNA.
DR EMBL; AF220759; AAF26446.1; -; mRNA.
DR PIR; T01489; T01489.
DR RefSeq; NP_564987.2; NM_105701.2.
DR AlphaFoldDB; Q8VXY7; -.
DR SMR; Q8VXY7; -.
DR STRING; 3702.AT1G70330.1; -.
DR PaxDb; Q8VXY7; -.
DR PRIDE; Q8VXY7; -.
DR ProteomicsDB; 220774; -.
DR EnsemblPlants; AT1G70330.1; AT1G70330.1; AT1G70330.
DR GeneID; 843369; -.
DR Gramene; AT1G70330.1; AT1G70330.1; AT1G70330.
DR KEGG; ath:AT1G70330; -.
DR Araport; AT1G70330; -.
DR TAIR; locus:2016119; AT1G70330.
DR eggNOG; KOG1479; Eukaryota.
DR HOGENOM; CLU_021611_5_0_1; -.
DR InParanoid; Q8VXY7; -.
DR OMA; FWNLGDL; -.
DR OrthoDB; 674800at2759; -.
DR PhylomeDB; Q8VXY7; -.
DR PRO; PR:Q8VXY7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VXY7; baseline and differential.
DR Genevisible; Q8VXY7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR InterPro; IPR034764; ENT1/ENT2.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR Pfam; PF01733; Nucleoside_tran; 1.
DR PIRSF; PIRSF016379; ENT; 1.
DR PRINTS; PR01130; DERENTRNSPRT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00939; 2a57; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..450
FT /note="Equilibrative nucleotide transporter 1"
FT /id="PRO_0000419154"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 111
FT /note="L -> P (in Ref. 4; AAF26446)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="M -> T (in Ref. 4; AAF26446)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 450 AA; 49344 MW; E82337DE5AA158B9 CRC64;
MTPISQRISQ KSPKLRETKQ IQMTTTDKSA GIVTDSEAGP ETSLLLNPHE GSTKKAPSDS
YHFAYIIYFT LGVGFLLPWN AFITAVDYFS YLYPSTAVDR IFAVIYMLVA LVCLFVIVVF
YAHKSLASFR INLGLLLFVI ALLVVPVLDL VYVKGQVGLY AGFDVTSAAV ALSGLGDALM
QGGLIGVAGE MPERYMQAVV AGTAGSGVLV SLLRILTKAV YPQDPDGLRK SANLYFAVGI
VVMVICAVFY NVAHKLPVIK FHEERKNEEL IREKSEEKGS LTGLAWRTTL WDIVTKVKSH
GFGIVLLYMV TLSIFPGYIT EDVHSELLTD WYPILLIAAY NVFDLVGKCL TAVFMLEDEK
IAVGGSIARL LFYPLFWGCL HGPMFLRTEI PVTILTCLLG LTNGYLTSVL MILAPKSVPL
RHSETAGIVT VMFLVVGLAS GSVIAWFWVI
