ENT2_ARATH
ID ENT2_ARATH Reviewed; 417 AA.
AC Q9SR64; Q944N9;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Equilibrative nucleotide transporter 2;
DE Short=AtENT2;
DE AltName: Full=Nucleoside transporter ENT2;
GN Name=ENT2; OrderedLocusNames=At3g09990; ORFNames=T22K18.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12810710; DOI=10.1074/jbc.m304768200;
RA Li G., Liu K., Baldwin S.A., Wang D.;
RT "Equilibrative nucleoside transporters of Arabidopsis thaliana. cDNA
RT cloning, expression pattern, and analysis of transport activities.";
RL J. Biol. Chem. 278:35732-35742(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May be involved in nucleoside transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}. Note=Plasma membrane.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and flowers.
CC {ECO:0000269|PubMed:12810710}.
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
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DR EMBL; AF426399; AAL25095.1; -; mRNA.
DR EMBL; AC010927; AAF04424.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74845.1; -; Genomic_DNA.
DR RefSeq; NP_187610.1; NM_111834.1.
DR AlphaFoldDB; Q9SR64; -.
DR SMR; Q9SR64; -.
DR STRING; 3702.AT3G09990.1; -.
DR PaxDb; Q9SR64; -.
DR PRIDE; Q9SR64; -.
DR EnsemblPlants; AT3G09990.1; AT3G09990.1; AT3G09990.
DR GeneID; 820160; -.
DR Gramene; AT3G09990.1; AT3G09990.1; AT3G09990.
DR KEGG; ath:AT3G09990; -.
DR Araport; AT3G09990; -.
DR TAIR; locus:2100068; AT3G09990.
DR eggNOG; KOG1479; Eukaryota.
DR HOGENOM; CLU_021611_5_1_1; -.
DR InParanoid; Q9SR64; -.
DR OMA; HNEAKIN; -.
DR OrthoDB; 674800at2759; -.
DR PhylomeDB; Q9SR64; -.
DR PRO; PR:Q9SR64; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SR64; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR InterPro; IPR030200; ENT2_plant.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR PANTHER; PTHR10332:SF30; PTHR10332:SF30; 1.
DR Pfam; PF01733; Nucleoside_tran; 1.
DR PIRSF; PIRSF016379; ENT; 1.
DR PRINTS; PR01130; DERENTRNSPRT.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..417
FT /note="Equilibrative nucleotide transporter 2"
FT /id="PRO_0000419155"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 187
FT /note="F -> L (in Ref. 1; AAL25095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 417 AA; 45676 MW; 7F808E3169889755 CRC64;
MDTSILAVTT NPKGKNYALA VCWLLGVGCL LAWNSMLTIV DYYAYLFPWY HPSRILTIIY
QSFSIGALSV LVHKEARLNT RRRNLFGYSL FSLGSLAVLV LNLATSGRGG IGSFIGVCVI
SAAFGLADAH VYGGMIGDLS MMTPEFLQSF LAGLAASGAL TSGLRLVIKA AFKNSRDGLR
KGATLFFAMS ASFELVCVLL YAYVFPRIPV VKYYRAKAII QGSRTVWADL AAGGIQVQPI
TQDEEALRYD HRLNKGDLML LYSDLAVTLF LVYLLTFSIF PGFLSEDTGK YSLGDWYALV
LIAVFNVSDL VGRYVPMVKK LKMKSRKCLL ITSLGRLLLI PAFNITGIYG SQGWMIFLMS
VLGLSNGYLT VCVITSAPYD LLAPEQNALG NLLVLYICGG MFAGVACDWL WLVGKDW
