ENT4_YEAST
ID ENT4_YEAST Reviewed; 247 AA.
AC Q07872; D6VXW8;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Epsin-4;
GN Name=ENT4; OrderedLocusNames=YLL038C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- MISCELLANEOUS: Present with 1960 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z73143; CAA97488.1; -; Genomic_DNA.
DR EMBL; AY558191; AAS56517.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09284.1; -; Genomic_DNA.
DR PIR; S64789; S64789.
DR RefSeq; NP_013062.1; NM_001181858.1.
DR AlphaFoldDB; Q07872; -.
DR SMR; Q07872; -.
DR BioGRID; 31216; 199.
DR DIP; DIP-2685N; -.
DR IntAct; Q07872; 5.
DR MINT; Q07872; -.
DR STRING; 4932.YLL038C; -.
DR iPTMnet; Q07872; -.
DR MaxQB; Q07872; -.
DR PaxDb; Q07872; -.
DR PRIDE; Q07872; -.
DR TopDownProteomics; Q07872; -.
DR EnsemblFungi; YLL038C_mRNA; YLL038C; YLL038C.
DR GeneID; 850621; -.
DR KEGG; sce:YLL038C; -.
DR SGD; S000003961; ENT4.
DR VEuPathDB; FungiDB:YLL038C; -.
DR eggNOG; KOG2056; Eukaryota.
DR HOGENOM; CLU_053580_1_0_1; -.
DR InParanoid; Q07872; -.
DR OMA; NEFVAWI; -.
DR BioCyc; YEAST:G3O-32140-MON; -.
DR Reactome; R-SCE-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR PRO; PR:Q07872; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q07872; protein.
DR GO; GO:0030479; C:actin cortical patch; TAS:SGD.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0030276; F:clathrin binding; ISS:SGD.
DR GO; GO:0000147; P:actin cortical patch assembly; ISS:SGD.
DR GO; GO:0007015; P:actin filament organization; ISS:SGD.
DR GO; GO:0006897; P:endocytosis; ISS:SGD.
DR GO; GO:0072659; P:protein localization to plasma membrane; IMP:SGD.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR008942; ENTH_VHS.
DR Pfam; PF01417; ENTH; 1.
DR SMART; SM00273; ENTH; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50942; ENTH; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..247
FT /note="Epsin-4"
FT /id="PRO_0000074527"
FT DOMAIN 7..146
FT /note="ENTH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT REGION 179..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..200
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 195
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 247 AA; 28271 MW; F5C95AA2402918D0 CRC64;
MPLLDTFKSF IQSPTESKVK QATNEDETSG ATGTLMNEIS ILTYSPKTVR EIIQVIRKRL
LLGQNRRNSH RNCIQVMKTL TLVSYLMNNG SNEFIKWLKG NMILIEILED FQVQDPRDER
KAEDIQKLSR NVLGLLQDDG LLEKQRKDVI QFRSSISTPG RKSTDNSHLK LEEMRSELTR
QSLEKKAKPP TTSTSLDFQR QRTRNTHEYA RFSLDPLAEE DSEDTPGVAG GISKLSFRPK
SSNNPFR
