ENT7_ARATH
ID ENT7_ARATH Reviewed; 417 AA.
AC Q944P0; Q9SY87;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Equilibrative nucleotide transporter 7;
DE Short=AtENT7;
DE AltName: Full=Nucleoside transporter ENT7;
GN Name=ENT7; OrderedLocusNames=At1g61630; ORFNames=T25B24.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=12810710; DOI=10.1074/jbc.m304768200;
RA Li G., Liu K., Baldwin S.A., Wang D.;
RT "Equilibrative nucleoside transporters of Arabidopsis thaliana. cDNA
RT cloning, expression pattern, and analysis of transport activities.";
RL J. Biol. Chem. 278:35732-35742(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=15228386; DOI=10.1042/bj20040389;
RA Wormit A., Traub M., Floerchinger M., Neuhaus H.E., Moehlmann T.;
RT "Characterization of three novel members of the Arabidopsis thaliana
RT equilibrative nucleoside transporter (ENT) family.";
RL Biochem. J. 383:19-26(2004).
CC -!- FUNCTION: Nucleoside transporter that can mediate uptake of adenosine,
CC uridine, guanosine or cytidine when expressed in a heterologous system
CC (yeast). {ECO:0000269|PubMed:15228386}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=9.8 uM for adenosine {ECO:0000269|PubMed:15228386};
CC KM=13.4 uM for uridine {ECO:0000269|PubMed:15228386};
CC KM=9.4 uM for guanosine {ECO:0000269|PubMed:15228386};
CC KM=40 uM for cytidine {ECO:0000269|PubMed:15228386};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}. Note=Plasma membrane.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and flowers.
CC {ECO:0000269|PubMed:12810710}.
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25545.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF426398; AAL25094.1; -; mRNA.
DR EMBL; AC005850; AAD25545.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33864.1; -; Genomic_DNA.
DR EMBL; DQ446383; ABE65731.1; -; mRNA.
DR PIR; G96641; G96641.
DR RefSeq; NP_176357.2; NM_104845.3.
DR AlphaFoldDB; Q944P0; -.
DR SMR; Q944P0; -.
DR STRING; 3702.AT1G61630.1; -.
DR TCDB; 2.A.57.1.10; the equilibrative nucleoside transporter (ent) family.
DR PaxDb; Q944P0; -.
DR PRIDE; Q944P0; -.
DR ProteomicsDB; 221900; -.
DR EnsemblPlants; AT1G61630.1; AT1G61630.1; AT1G61630.
DR GeneID; 842459; -.
DR Gramene; AT1G61630.1; AT1G61630.1; AT1G61630.
DR KEGG; ath:AT1G61630; -.
DR Araport; AT1G61630; -.
DR TAIR; locus:2200893; AT1G61630.
DR eggNOG; KOG1479; Eukaryota.
DR HOGENOM; CLU_021611_5_1_1; -.
DR InParanoid; Q944P0; -.
DR OMA; MNIFMIY; -.
DR OrthoDB; 559763at2759; -.
DR PhylomeDB; Q944P0; -.
DR PRO; PR:Q944P0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q944P0; baseline and differential.
DR Genevisible; Q944P0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015858; P:nucleoside transport; IDA:UniProtKB.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR Pfam; PF01733; Nucleoside_tran; 1.
DR PIRSF; PIRSF016379; ENT; 1.
DR PRINTS; PR01130; DERENTRNSPRT.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..417
FT /note="Equilibrative nucleotide transporter 7"
FT /id="PRO_0000419160"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 417 AA; 45735 MW; 7ECD5783F38F9B6C CRC64;
MTNPEDIPSR IEGKNVARLV CCFLGVGSLV AWNAMLTITD YYYQLFPKYH PSRVLTIVYQ
LVANVFIITL ATKEAKLNTR LRNIFGYSLY TAGTFCLIIL DLASHGSGSV VAYVLLCLIV
ALFGLADAFV QGAMVGDLSF MSPDFIQAFM AGLGIAGALT SVLRLITKAI FDNSPDGLRK
GALLFIGIAT LIELACVFLY TLVFAKLPIV KYYRAKAGKE GAKTVSADLA AAGLQEQAEQ
VHQMDESKIQ KLTKKQLLRE NIDLGINLSL IYVVTLSIFP GFLYENTGEH RLGDWYAPVL
VAMYNGWDAI SRFIPSIKPL AMESRKWITV CVVARLLLVP AFYFTAKYAD QGWMLFLTSF
LGLSNGYLTV CIFSTAPKGY NGPEANALGN LMCVFLLGGI FAGVCLGWLW LIGNDSF
