ENT8_ARATH
ID ENT8_ARATH Reviewed; 389 AA.
AC Q84XI3; Q7XAU2; Q9FWY1;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Equilibrative nucleotide transporter 8;
DE Short=AtENT8;
DE AltName: Full=Nucleoside transporter ENT8;
GN Name=ETN8; OrderedLocusNames=At1g02630; ORFNames=T14P4.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=12810710; DOI=10.1074/jbc.m304768200;
RA Li G., Liu K., Baldwin S.A., Wang D.;
RT "Equilibrative nucleoside transporters of Arabidopsis thaliana. cDNA
RT cloning, expression pattern, and analysis of transport activities.";
RL J. Biol. Chem. 278:35732-35742(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Li G., Liu K., Wang D.;
RT "ENT8 splice variant mRNA.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in nucleoside transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}. Note=Plasma membrane.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84XI3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84XI3-2; Sequence=VSP_044113;
CC -!- TISSUE SPECIFICITY: Expressed in stems, flowers and siliques.
CC {ECO:0000269|PubMed:12810710}.
CC -!- INDUCTION: By nitrogen deficiency and 5-fluorouracil plus methotrexate.
CC {ECO:0000269|PubMed:12810710}.
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG10625.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY187030; AAO31974.1; -; mRNA.
DR EMBL; AY341059; AAQ16125.1; -; mRNA.
DR EMBL; AC022521; AAG10625.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27451.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27452.1; -; Genomic_DNA.
DR EMBL; AK221480; BAD94637.1; -; mRNA.
DR PIR; C86156; C86156.
DR RefSeq; NP_001030934.1; NM_001035857.2. [Q84XI3-2]
DR RefSeq; NP_171763.1; NM_100143.3. [Q84XI3-1]
DR AlphaFoldDB; Q84XI3; -.
DR SMR; Q84XI3; -.
DR STRING; 3702.AT1G02630.1; -.
DR iPTMnet; Q84XI3; -.
DR PaxDb; Q84XI3; -.
DR PRIDE; Q84XI3; -.
DR ProteomicsDB; 220552; -. [Q84XI3-1]
DR EnsemblPlants; AT1G02630.1; AT1G02630.1; AT1G02630. [Q84XI3-1]
DR EnsemblPlants; AT1G02630.2; AT1G02630.2; AT1G02630. [Q84XI3-2]
DR GeneID; 839240; -.
DR Gramene; AT1G02630.1; AT1G02630.1; AT1G02630. [Q84XI3-1]
DR Gramene; AT1G02630.2; AT1G02630.2; AT1G02630. [Q84XI3-2]
DR KEGG; ath:AT1G02630; -.
DR Araport; AT1G02630; -.
DR TAIR; locus:2196070; AT1G02630.
DR eggNOG; KOG1479; Eukaryota.
DR HOGENOM; CLU_021611_5_0_1; -.
DR InParanoid; Q84XI3; -.
DR OMA; FDMSVVY; -.
DR PhylomeDB; Q84XI3; -.
DR PRO; PR:Q84XI3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84XI3; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR Pfam; PF01733; Nucleoside_tran; 2.
DR PIRSF; PIRSF016379; ENT; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..389
FT /note="Equilibrative nucleotide transporter 8"
FT /id="PRO_0000419161"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 242..285
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_044113"
SQ SEQUENCE 389 AA; 43345 MW; CE3949EC5A79D8BD CRC64;
MVDEKVIVDE VETRDAYRVA YVIHFLLGAG SLIPWNALIT AVDYFGYLYP DKHVEKTFTV
AYMSCSVLVL VLMMTWNTRM SYRVRMNLGF SMFIIAMMIS PLIDWVWKGE KGENVSYMLM
VGSVVLCGLA DGVVGGSLIG SAGKLPRQYM QAIFAGTASS GIIISLLRIA TKASLPQTPQ
GMRTSAHSYF IVSSTILLCC FISCNVLHKL PVMQQHLKFH QPLHSTLTIW MVGRKIKWPA
SGMLIIYSVT LSIFPGFIAE NLKSQLLQSW YPILLITVYN ISDFVGKSLT ALYLWQSIKS
ATWACIVRLL FYPLFSACLR GPKWLRTEVP VVVLTFMLGL TNGYLTSVLM IMAPKTVHAS
EAELAAIFMV VFLGLGLVCG SVIGWLWLI
