ID   ENTH_CROTZ              Reviewed;         137 AA.
AC   C9XXI1;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Proofreading thioesterase EntH {ECO:0000255|HAMAP-Rule:MF_00907};
DE            EC=3.1.2.- {ECO:0000255|HAMAP-Rule:MF_00907};
DE   AltName: Full=Enterobactin synthase component H {ECO:0000255|HAMAP-Rule:MF_00907};
GN   Name=entH {ECO:0000255|HAMAP-Rule:MF_00907}; OrderedLocusNames=Ctu_30430;
OS   Cronobacter turicensis (strain DSM 18703 / CCUG 55852 / LMG 23827 / z3032).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Cronobacter.
OX   NCBI_TaxID=693216;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18703 / CCUG 55852 / LMG 23827 / z3032;
RX   PubMed=21037008; DOI=10.1128/jb.01162-10;
RA   Stephan R., Lehner A., Tischler P., Rattei T.;
RT   "Complete genome sequence of Cronobacter turicensis LMG 23827, a food-borne
RT   pathogen causing deaths in neonates.";
RL   J. Bacteriol. 193:309-310(2011).
CC   -!- FUNCTION: Required for optimal enterobactin synthesis. Acts as a
CC       proofreading enzyme that prevents EntB misacylation by hydrolyzing the
CC       thioester bound existing between EntB and wrongly charged molecules.
CC       {ECO:0000255|HAMAP-Rule:MF_00907}.
CC   -!- PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00907}.
CC   -!- SUBUNIT: Homotetramer. Dimer of dimers. Interacts specifically with the
CC       aryl carrier protein (ArCP) domain of EntB. {ECO:0000255|HAMAP-
CC       Rule:MF_00907}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00907}.
CC   -!- SIMILARITY: Belongs to the thioesterase PaaI family.
CC       {ECO:0000255|HAMAP-Rule:MF_00907}.
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DR   EMBL; FN543093; CBA32696.1; -; Genomic_DNA.
DR   RefSeq; WP_015742038.1; NC_013282.2.
DR   AlphaFoldDB; C9XXI1; -.
DR   SMR; C9XXI1; -.
DR   EnsemblBacteria; CBA32696; CBA32696; CTU_30430.
DR   GeneID; 60373512; -.
DR   KEGG; ctu:CTU_30430; -.
DR   PATRIC; fig|693216.3.peg.2880; -.
DR   HOGENOM; CLU_089876_13_1_6; -.
DR   OMA; QHGGVYC; -.
DR   UniPathway; UPA00017; -.
DR   Proteomes; UP000002069; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016790; F:thiolester hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009239; P:enterobactin biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00907; Thioesterase_EntH; 1.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   InterPro; IPR003736; PAAI_dom.
DR   InterPro; IPR026576; Thioesterase_EntH.
DR   InterPro; IPR006683; Thioestr_dom.
DR   Pfam; PF03061; 4HBT; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR00369; unchar_dom_1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase.
FT   CHAIN           1..137
FT                   /note="Proofreading thioesterase EntH"
FT                   /id="PRO_0000413864"
FT   ACT_SITE        63
FT                   /note="Nucleophile or proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00907"
SQ   SEQUENCE   137 AA;  14878 MW;  E2C696AB06281528 CRC64;
     MIWKRHMTLE ALNATSVGTL VEHLGIEYTR LGDDLLEATM PVDTRTHQPF GLLHGGASAA
     LAETLGSMAG YLTTRDGQCV VGTEISASHH RAVSQGQVRG VCQPLHLGRQ SQCWEIVIYD
     EQGRRCCTSR LSTAVMG