ID   ENTH_KLEP7              Reviewed;         137 AA.
AC   A6T644;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Proofreading thioesterase EntH;
DE            EC=3.1.2.-;
DE   AltName: Full=Enterobactin synthase component H;
GN   Name=entH; OrderedLocusNames=KPN78578_06040; ORFNames=KPN_00615;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for optimal enterobactin synthesis. Acts as a
CC       proofreading enzyme that prevents EntB misacylation by hydrolyzing the
CC       thioester bound existing between EntB and wrongly charged molecules (By
CC       similarity). {ECO:0000250}.
CC   -!- PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis.
CC   -!- SUBUNIT: Homotetramer. Dimer of dimers. Interacts specifically with the
CC       aryl carrier protein (ArCP) domain of EntB. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thioesterase PaaI family. {ECO:0000305}.
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DR   EMBL; CP000647; ABR76065.1; -; Genomic_DNA.
DR   RefSeq; WP_008805589.1; NC_009648.1.
DR   AlphaFoldDB; A6T644; -.
DR   SMR; A6T644; -.
DR   STRING; 272620.KPN_00615; -.
DR   EnsemblBacteria; ABR76065; ABR76065; KPN_00615.
DR   GeneID; 56940074; -.
DR   KEGG; kpn:KPN_00615; -.
DR   HOGENOM; CLU_089876_13_1_6; -.
DR   OMA; QHGGVYC; -.
DR   UniPathway; UPA00017; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016790; F:thiolester hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009239; P:enterobactin biosynthetic process; IEA:UniProtKB-UniRule.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   InterPro; IPR003736; PAAI_dom.
DR   InterPro; IPR026576; Thioesterase_EntH.
DR   InterPro; IPR006683; Thioestr_dom.
DR   Pfam; PF03061; 4HBT; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR00369; unchar_dom_1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..137
FT                   /note="Proofreading thioesterase EntH"
FT                   /id="PRO_0000413870"
FT   ACT_SITE        63
FT                   /note="Nucleophile or proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   137 AA;  14799 MW;  6001456AB6424EC5 CRC64;
     MAWKRELTLA ALNASSENTM VAHLGIIYTR LEAGLLEAEM PVDARTHQPF GLLHGGASAA
     LAETLGSMAG WLMTEEGQCV VGTELNATHH RPVSSGKVRG ECRPLHLGRQ SQSWEIVVYD
     EKGRRCCTCR LGTAVLG