ENTS_ECOLI
ID ENTS_ECOLI Reviewed; 416 AA.
AC P24077; Q2MBL0;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Enterobactin exporter EntS {ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305};
DE AltName: Full=Protein p43;
GN Name=entS {ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000303|PubMed:12068807};
GN Synonyms=ybdA; OrderedLocusNames=b0591, JW0583;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1838574; DOI=10.1111/j.1365-2958.1991.tb00788.x;
RA Shea C.M., McIntosh M.A.;
RT "Nucleotide sequence and genetic organization of the ferric enterobactin
RT transport system: homology to other periplasmic binding protein-dependent
RT systems in Escherichia coli.";
RL Mol. Microbiol. 5:1415-1428(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1787794; DOI=10.1111/j.1365-2958.1991.tb00787.x;
RA Chenault S.S., Earhart C.F.;
RT "Organization of genes encoding membrane proteins of the Escherichia coli
RT ferrienterobactin permease.";
RL Mol. Microbiol. 5:1405-1413(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=BM694;
RX PubMed=12068807; DOI=10.1046/j.1365-2958.2002.02885.x;
RA Furrer J.L., Sanders D.N., Hook-Barnard I.G., McIntosh M.A.;
RT "Export of the siderophore enterobactin in Escherichia coli: involvement of
RT a 43 kDa membrane exporter.";
RL Mol. Microbiol. 44:1225-1234(2002).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [8]
RP FUNCTION.
RX PubMed=22952739; DOI=10.1371/journal.pone.0043700;
RA Koita K., Rao C.V.;
RT "Identification and analysis of the putative pentose sugar efflux
RT transporters in Escherichia coli.";
RL PLoS ONE 7:E43700-E43700(2012).
CC -!- FUNCTION: Component of an export pathway for enterobactin
CC (PubMed:12068807). Overexpression reduces intracellular arabinose
CC concentrations (PubMed:22952739). {ECO:0000269|PubMed:12068807,
CC ECO:0000269|PubMed:22952739}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01436, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_01436}.
CC -!- DISRUPTION PHENOTYPE: Mutant is unable to secrete enterobactin
CC efficiently. It secretes little, if any, enterobactin, but elevated
CC levels of enterobactin breakdown products 2,3-dihydroxybenzoylserine
CC (DHBS) monomer, dimer and trimer. {ECO:0000269|PubMed:12068807}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. EntS (TC
CC 2.A.1.38) family. {ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305}.
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DR EMBL; X57470; CAA40706.1; -; Genomic_DNA.
DR EMBL; X59402; CAA42044.1; -; Genomic_DNA.
DR EMBL; U82598; AAB40790.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73692.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76346.1; -; Genomic_DNA.
DR PIR; S16306; S16306.
DR RefSeq; NP_415123.1; NC_000913.3.
DR RefSeq; WP_001041786.1; NZ_SSZK01000032.1.
DR AlphaFoldDB; P24077; -.
DR SMR; P24077; -.
DR BioGRID; 4260903; 384.
DR STRING; 511145.b0591; -.
DR TCDB; 2.A.1.38.1; the major facilitator superfamily (mfs).
DR PaxDb; P24077; -.
DR PRIDE; P24077; -.
DR EnsemblBacteria; AAC73692; AAC73692; b0591.
DR EnsemblBacteria; BAE76346; BAE76346; BAE76346.
DR GeneID; 946268; -.
DR KEGG; ecj:JW0583; -.
DR KEGG; eco:b0591; -.
DR PATRIC; fig|1411691.4.peg.1678; -.
DR EchoBASE; EB1096; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_034180_11_0_6; -.
DR InParanoid; P24077; -.
DR OMA; TPDDMRG; -.
DR PhylomeDB; P24077; -.
DR BioCyc; EcoCyc:YBDA-MON; -.
DR BioCyc; MetaCyc:YBDA-MON; -.
DR PRO; PR:P24077; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0042931; F:enterobactin transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:EcoCyc.
DR GO; GO:0042930; P:enterobactin transport; IMP:EcoliWiki.
DR GO; GO:0046677; P:response to antibiotic; IMP:EcoCyc.
DR Gene3D; 1.20.1250.20; -; 1.
DR HAMAP; MF_01436; MFS_EntS; 1.
DR InterPro; IPR023722; Enterobactin_exp_EntS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR010290; TM_effector.
DR Pfam; PF05977; MFS_3; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..416
FT /note="Enterobactin exporter EntS"
FT /id="PRO_0000168661"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 43..55
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 77..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 105..109
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 131..156
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 178
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 200..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 240..256
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 278..287
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 288..307
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 308..313
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 314..336
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 337..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 378
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436, ECO:0000305"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436"
FT TOPO_DOM 400..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01436,
FT ECO:0000269|PubMed:15919996"
FT CONFLICT 123
FT /note="F -> S (in Ref. 1; CAA40706)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 43282 MW; 64FCCF9AF99AC25A CRC64;
MNKQSWLLNL SLLKTHPAFR AVFLARFISI VSLGLLGVAV PVQIQMMTHS TWQVGLSVTL
TGGAMFVGLM VGGVLADRYE RKKVILLARG TCGIGFIGLC LNALLPEPSL LAIYLLGLWD
GFFASLGVTA LLAATPALVG RENLMQAGAI TMLTVRLGSV ISPMIGGLLL ATGGVAWNYG
LAAAGTFITL LPLLSLPALP PPPQPREHPL KSLLAGFRFL LASPLVGGIA LLGGLLTMAS
AVRVLYPALA DNWQMSAAQI GFLYAAIPLG AAIGALTSGK LAHSARPGLL MLLSTLGSFL
AIGLFGLMPM WILGVVCLAL FGWLSAVSSL LQYTMLQTQT PEAMLGRING LWTAQNVTGD
AIGAALLGGL GAMMTPVASA SASGFGLLII GVLLLLVLVE LRHFRQTPPQ VTASDS
