AGN1_PAEDI
ID AGN1_PAEDI Reviewed; 149 AA.
AC A0A411PQP6;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 08-MAY-2019, sequence version 1.
DT 25-MAY-2022, entry version 11.
DE RecName: Full=Decarboxylase AgnL1 {ECO:0000303|PubMed:30746079};
DE EC=4.1.1.- {ECO:0000305|PubMed:30746079};
DE AltName: Full=Agnestins biosynthesis cluster protein L1 {ECO:0000303|PubMed:30746079};
GN Name=AgnL1 {ECO:0000303|PubMed:30746079};
OS Paecilomyces divaricatus (Penicillium divaricatum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Thermoascaceae; Paecilomyces.
OX NCBI_TaxID=644132;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC STRAIN=K5013;
RX PubMed=30746079; DOI=10.1039/c8sc03778g;
RA Szwalbe A.J., Williams K., Song Z., de Mattos-Shipley K., Vincent J.L.,
RA Bailey A.M., Willis C.L., Cox R.J., Simpson T.J.;
RT "Characterisation of the biosynthetic pathway to agnestins A and B reveals
RT the reductive route to chrysophanol in fungi.";
RL Chem. Sci. 10:233-238(2019).
CC -!- FUNCTION: Decarboxylase; part of the gene cluster that mediates the
CC biosynthesis of agnestins, dihydroxy-xanthone metabolites
CC (PubMed:30746079). The pathway begins with the assembly and cyclization
CC of atrochrysone thioester by the non-reducing polyketide synthase
CC Agnpks1 (PubMed:30746079). The atrochrysone carboxyl ACP thioesterase
CC AgnL7 then breaks the thioester bond and releases the atrochrysone
CC carboxylic acid as the first enzyme-free intermediate
CC (PubMed:30746079). The decarboxylase AgnL1 then catalyzes the concerted
CC decarboxylation-elimination required to convert atochrysone carboxylic
CC acid into emodin anthrone, which is further oxidized to emodin by the
CC anthrone oxygenase AgnL2 (PubMed:30746079). Emodin then undergoes
CC reduction catalyzed by the oxidoreductase AgnL4 to yield the
CC dihydroquinone tautomer which is the substrate for reduction by the
CC short chain dehydrogenase AgnL6 reduction to produce hydroxyketone,
CC followed by AgnL8 dehydration and likely spontaneous autoxidation to
CC chrysophanol (PubMed:30746079). Baeyer-Villiger oxidation by the
CC oxidase AgnL3 leads to monodictyphenone via cleavage of the C-10/C-10a
CC bond of chrysophanol (PubMed:30746079). Alternative cleavage at the C-
CC 4a/C-10 bond of chrysophanol leads also to the formation some cephalone
CC F (PubMed:30746079). Further conversion to agnestins A and B, requires
CC reduction to dihydro-monodictyphenone, oxidation to agnestin C probably
CC via an epoxide, and rearrangement to either agnestin A or agnestin B
CC directly, although agnestin A or agnestin B can also interconvert
CC (PubMed:30746079). Within the cluster, AgnR1 is the only unassigned
CC oxidoreductase present which could be involved in this conversion.
CC However, AgnR1 seems not to be involved in this step, and thus genes
CC involved in the proposed oxidation/reduction may be located elsewhere
CC on the genome (PubMed:30746079). Further agnestin A derivatives are
CC probably formed by spontaneous decarboxylations, dehydrations and
CC methanolysis reactions (PubMed:30746079).
CC {ECO:0000269|PubMed:30746079}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=atrochrysone carboxylate + H(+) = atrochrysone + CO2;
CC Xref=Rhea:RHEA:64264, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:149713, ChEBI:CHEBI:150016;
CC Evidence={ECO:0000305|PubMed:30746079};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64265;
CC Evidence={ECO:0000305|PubMed:30746079};
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:30746079}.
CC -!- SIMILARITY: Belongs to the tpcK family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MH898872; QBG38887.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A411PQP6; -.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR InterPro; IPR011008; Dimeric_a/b-barrel.
DR InterPro; IPR009799; EthD_dom.
DR Pfam; PF07110; EthD; 1.
DR SUPFAM; SSF54909; SSF54909; 1.
PE 3: Inferred from homology;
KW Lyase.
FT CHAIN 1..149
FT /note="Decarboxylase AgnL1"
FT /id="PRO_0000449012"
FT DOMAIN 30..125
FT /note="EthD"
FT /evidence="ECO:0000255"
SQ SEQUENCE 149 AA; 17303 MW; 8C55EC32A3EEC7D3 CRC64;
MPDASEIPPP PPGAPGTQFL CLTICGYRRP GMSEEDYRHH MTQVSAPMTQ DLMVKYGVKR
WTMVHNTSET RALMSRLFDH QMTQLADFDC FSQVVFESVE EYRRMKEDAW YQEHLVGDHE
KFADTQRSRM TLGWITELIR DGKVVKGTQ