ID   AGN3_APLCA              Reviewed;         108 AA.
AC   P01364;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Abdominal ganglion neuropeptide R3-14;
DE   Contains:
DE     RecName: Full=Histidine-rich basic peptide;
DE              Short=HRBP;
DE   Contains:
DE     RecName: Full=Peptide I;
DE   Contains:
DE     RecName: Full=Peptide II;
DE   Flags: Precursor;
OS   Aplysia californica (California sea hare).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC   Aplysiidae; Aplysia.
OX   NCBI_TaxID=6500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6194901; DOI=10.1016/0092-8674(83)90206-4;
RA   Nambu J.R., Taussig R., Mahon A.C., Scheller R.H.;
RT   "Gene isolation with cDNA probes from identified Aplysia neurons:
RT   neuropeptide modulators of cardiovascular physiology.";
RL   Cell 35:47-56(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6474178; DOI=10.1126/science.6474178;
RA   Scheller R.H., Kaldany R.-R., Kreiner T., Mahon A.C., Nambu J.R.,
RA   Schaefer M., Taussig R.;
RT   "Neuropeptides: mediators of behavior in Aplysia.";
RL   Science 225:1300-1308(1984).
RN   [3]
RP   PROTEIN SEQUENCE OF 24-54 AND 66-108, AND PYROGLUTAMATE FORMATION AT
RP   GLN-66.
RC   TISSUE=Abdominal ganglion;
RX   PubMed=2573895; DOI=10.1016/0196-9781(89)90124-1;
RA   Nagle G.T., Knock S.L., Painter S.D., Blankenship J.E., Fritz R.R.,
RA   Kurosky A.;
RT   "Aplysia californica neurons R3-R14: primary structure of the myoactive
RT   histidine-rich basic peptide and peptide I.";
RL   Peptides 10:849-857(1989).
RN   [4]
RP   PROTEOLYTIC PROCESSING.
RX   PubMed=3549995; DOI=10.1523/jneurosci.07-03-00854.1987;
RA   Newcomb R., Scheller R.H.;
RT   "Proteolytic processing of the Aplysia egg-laying hormone and R3-14
RT   neuropeptide precursors.";
RL   J. Neurosci. 7:854-863(1987).
RN   [5]
RP   PYROGLUTAMATE FORMATION AT GLU-24 AND GLU-52.
RX   PubMed=9930740; DOI=10.1046/j.1471-4159.1999.0720676.x;
RA   Garden R.W., Moroz T.P., Gleeson J.M., Floyd P.D., Li L., Rubakhin S.S.,
RA   Sweedler J.V.;
RT   "Formation of N-pyroglutamyl peptides from N-Glu and N-Gln precursors in
RT   Aplysia neurons.";
RL   J. Neurochem. 72:676-681(1999).
CC   -!- FUNCTION: HRBP is a myoactive peptide that excites Aplysia heart and
CC       enhances gut motility in vitro.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Neurons R3-R14. A cluster of 12 giant neurons
CC       located on the right side of the abdominal ganglion.
CC   -!- PTM: The partial formation of pyroglutamate from N-terminal glutamic
CC       acid in peptides isolated from single cells is detected by mass
CC       spectrometry (PubMed:9930740). There are indications this modification
CC       depends on a heat sensitive factor. {ECO:0000269|PubMed:9930740}.
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DR   EMBL; K01223; AAA27759.1; -; mRNA.
DR   EMBL; K02184; AAA27763.1; -; mRNA.
DR   EMBL; M64342; AAA27760.1; -; Genomic_DNA.
DR   EMBL; M64343; AAA27761.1; -; mRNA.
DR   PIR; A01635; NLGAAA.
DR   RefSeq; NP_001191478.1; NM_001204549.1.
DR   AlphaFoldDB; P01364; -.
DR   GeneID; 100533236; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Neuropeptide; Pyrrolidone carboxylic acid; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:2573895"
FT   PEPTIDE         24..49
FT                   /note="Peptide I"
FT                   /evidence="ECO:0000269|PubMed:2573895"
FT                   /id="PRO_0000001782"
FT   PEPTIDE         52..63
FT                   /note="Peptide II"
FT                   /id="PRO_0000001783"
FT   PEPTIDE         66..108
FT                   /note="Histidine-rich basic peptide"
FT                   /evidence="ECO:0000269|PubMed:2573895"
FT                   /id="PRO_0000001784"
FT   MOD_RES         24
FT                   /note="Pyrrolidone carboxylic acid (Glu); partial"
FT                   /evidence="ECO:0000269|PubMed:9930740"
FT   MOD_RES         52
FT                   /note="Pyrrolidone carboxylic acid (Glu); partial"
FT                   /evidence="ECO:0000269|PubMed:9930740"
FT   MOD_RES         66
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:2573895"
SQ   SEQUENCE   108 AA;  12207 MW;  621C72C17BCC1373 CRC64;
     MQVLHLCLAV SIAVALLSQA AWSEEVFDDT DVGDELTNAL ESVLTDFKDK REAEEPSAFM
     TRLRRQVAQM HVWRAVNHDR NHGTGSGRHG RFLIRNRYRY GGGHLSDA