AGN5_APLCA
ID AGN5_APLCA Reviewed; 112 AA.
AC P07712;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Abdominal ganglion neuropeptides L5-67;
DE Contains:
DE RecName: Full=Luqin;
DE Contains:
DE RecName: Full=Luqin-B;
DE Contains:
DE RecName: Full=Luqin-C;
DE Contains:
DE RecName: Full=Proline-rich mature peptide;
DE Short=PRMP;
DE Flags: Precursor;
OS Aplysia californica (California sea hare).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC Aplysiidae; Aplysia.
OX NCBI_TaxID=6500;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3013547; DOI=10.1089/dna.1986.5.203;
RA Shyamala M., Fisher J.M., Scheller R.H.;
RT "A neuropeptide precursor expressed in Aplysia neuron L5.";
RL DNA 5:203-208(1986).
RN [2]
RP CHARACTERIZATION, PROTEIN SEQUENCE OF 24-33, AND AMIDATION AT PHE-33.
RX PubMed=7784264; DOI=10.1016/0196-9781(94)00140-5;
RA Aloyz R.S., DesGroseillers L.;
RT "Processing of the L5-67 precursor peptide and characterization of LUQIN in
RT the LUQ neurons of Aplysia californica.";
RL Peptides 16:331-338(1995).
RN [3]
RP MASS SPECTROMETRY, AND PROTEOLYTIC PROCESSING.
RX PubMed=9809658; DOI=10.1016/s0196-9781(98)00094-1;
RA Li L., Moroz T.P., Garden R.W., Floyd P.D., Weiss K.R., Sweedler J.V.;
RT "Mass spectrometric survey of interganglionically transported peptides in
RT Aplysia.";
RL Peptides 19:1425-1433(1998).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Neurons L2-4 and L6, also called giant dorsal LUQ
CC (Left Upper Quadrant) neurons of the abdominal ganglion. Also expressed
CC in smaller neurons in the CNS and in peripheral organs such as the
CC kidney.
CC -!- PTM: The prohormone is proteolytically cleaved in 2 steps, yielding
CC first 2 products: luqin and PRMP. In the second step, PRMP is cleaved
CC to yield luqin-B and luqin-C. {ECO:0000269|PubMed:9809658}.
CC -!- MASS SPECTROMETRY: [Luqin]: Mass=1200.8; Mass_error=0.0002;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Proline-rich mature peptide]: Mass=8555;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Luqin-B]: Mass=3221.9; Mass_error=0.0003;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Luqin-C]: Mass=5354; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9809658};
CC -!- CAUTION: Was previously thought to be expressed in L5. {ECO:0000305}.
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DR EMBL; M13649; AAA27764.1; -; mRNA.
DR PIR; A25458; A25458.
DR RefSeq; NP_001191480.1; NM_001204551.1.
DR AlphaFoldDB; P07712; -.
DR GeneID; 100533239; -.
DR OrthoDB; 1625203at2759; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR016535; RFamide_neuropeptide_ACEP-1.
DR PIRSF; PIRSF008109; RFamide_neuropeptide_ACEP-1; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:7784264"
FT PEPTIDE 24..33
FT /note="Luqin"
FT /id="PRO_0000001788"
FT PEPTIDE 37..112
FT /note="Proline-rich mature peptide"
FT /id="PRO_0000001789"
FT PEPTIDE 37..65
FT /note="Luqin-B"
FT /id="PRO_0000001790"
FT PEPTIDE 66..112
FT /note="Luqin-C"
FT /id="PRO_0000001791"
FT MOD_RES 33
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7784264"
SQ SEQUENCE 112 AA; 12447 MW; A110C859028958C4 CRC64;
MKTAVLLVCL AYVMAAILSL CASAPSWRPQ GRFGKRTIPD RLPQTEESSL PDFGFSHLPA
LPLELFYNPR DLVHSGFRPR LCSVSGVEGY PPCVESHSDR KMKNLLDDLF GL
