AGN8_PAEDI
ID AGN8_PAEDI Reviewed; 174 AA.
AC A0A411PQN4;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 08-MAY-2019, sequence version 1.
DT 03-AUG-2022, entry version 9.
DE RecName: Full=Dehydratase AgnL8 {ECO:0000303|PubMed:30746079};
DE EC=4.2.1.- {ECO:0000305|PubMed:30746079};
DE AltName: Full=Agnestins biosynthesis cluster protein L8 {ECO:0000303|PubMed:30746079};
GN Name=AgnL8 {ECO:0000303|PubMed:30746079};
OS Paecilomyces divaricatus (Penicillium divaricatum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Thermoascaceae; Paecilomyces.
OX NCBI_TaxID=644132;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC STRAIN=K5013;
RX PubMed=30746079; DOI=10.1039/c8sc03778g;
RA Szwalbe A.J., Williams K., Song Z., de Mattos-Shipley K., Vincent J.L.,
RA Bailey A.M., Willis C.L., Cox R.J., Simpson T.J.;
RT "Characterisation of the biosynthetic pathway to agnestins A and B reveals
RT the reductive route to chrysophanol in fungi.";
RL Chem. Sci. 10:233-238(2019).
CC -!- FUNCTION: Dehydratase; part of the gene cluster that mediates the
CC biosynthesis of agnestins, dihydroxy-xanthone metabolites
CC (PubMed:30746079). The pathway begins with the assembly and cyclization
CC of atrochrysone thioester by the non-reducing polyketide synthase
CC Agnpks1 (PubMed:30746079). The atrochrysone carboxyl ACP thioesterase
CC AgnL7 then breaks the thioester bond and releases the atrochrysone
CC carboxylic acid as the first enzyme-free intermediate
CC (PubMed:30746079). The decarboxylase AgnL1 then catalyzes the concerted
CC decarboxylation-elimination required to convert atochrysone carboxylic
CC acid into emodin anthrone, which is further oxidized to emodin by the
CC anthrone oxygenase AgnL2 (PubMed:30746079). Emodin then undergoes
CC reduction catalyzed by the oxidoreductase AgnL4 to yield the
CC dihydroquinone tautomer which is the substrate for reduction by the
CC short chain dehydrogenase AgnL6 reduction to produce hydroxyketone,
CC followed by AgnL8 dehydration and likely spontaneous autoxidation to
CC chrysophanol (PubMed:30746079). Baeyer-Villiger oxidation by the
CC oxidase AgnL3 leads to monodictyphenone via cleavage of the C-10/C-10a
CC bond of chrysophanol (PubMed:30746079). Alternative cleavage at the C-
CC 4a/C-10 bond of chrysophanol leads also to the formation some cephalone
CC F (PubMed:30746079). Further conversion to agnestins A and B, requires
CC reduction to dihydro-monodictyphenone, oxidation to agnestin C probably
CC via an epoxide, and rearrangement to either agnestin A or agnestin B
CC directly, although agnestin A or agnestin B can also interconvert
CC (PubMed:30746079). Within the cluster, AgnR1 is the only unassigned
CC oxidoreductase present which could be involved in this conversion.
CC However, AgnR1 seems not to be involved in this step, and thus genes
CC involved in the proposed oxidation/reduction may be located elsewhere
CC on the genome (PubMed:30746079). Further agnestin A derivatives are
CC probably formed by spontaneous decarboxylations, dehydrations and
CC methanolysis reactions (PubMed:30746079).
CC {ECO:0000269|PubMed:30746079}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:30746079}.
CC -!- SUBUNIT: Homotrimer (By similarity). Each subunit contains an active
CC site, located in the central part of the hydrophobic core of the
CC monomer, which functions independently (By similarity).
CC {ECO:0000250|UniProtKB:P56221}.
CC -!- SIMILARITY: Belongs to the scytalone dehydratase family. {ECO:0000305}.
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DR EMBL; MH898872; QBG38880.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A411PQN4; -.
DR SMR; A0A411PQN4; -.
DR GO; GO:0030411; F:scytalone dehydratase activity; IEA:InterPro.
DR GO; GO:0006582; P:melanin metabolic process; IEA:InterPro.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR004235; Scytalone_dehydratase.
DR Pfam; PF02982; Scytalone_dh; 1.
DR PIRSF; PIRSF024851; SCD1; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 3: Inferred from homology;
KW Lyase.
FT CHAIN 1..174
FT /note="Dehydratase AgnL8"
FT /id="PRO_0000449020"
FT ACT_SITE 79
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT ACT_SITE 104
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 24
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 44
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 47
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
SQ SEQUENCE 174 AA; 20200 MW; 2B3D5515C10EFA55 CRC64;
MAKDPTFEEV TGCQRALFEW ADSYDTKDWE RLKKCVAPTL RIDYRSFLDK LWEAMPSDEF
ILMASDPRFL GNPLLKTQHF VGLSTWQKIS PDEIEGTHQL RVPHQRYTDS NMKEVAVKGH
AHGIATMWYK RVEDEWKFAG VCPQIRWAEF DYDKVFAEGK DHFGENGGNG EHAV