AGNO_POVBK
ID AGNO_POVBK Reviewed; 66 AA.
AC P03085;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 02-JUN-2021, entry version 82.
DE RecName: Full=Agnoprotein;
DE AltName: Full=Agno;
OS BK polyomavirus (BKPyV) (Human polyomavirus 1).
OC Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes;
OC Sepolyvirales; Polyomaviridae; Betapolyomavirus.
OX NCBI_TaxID=1891762;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Dunlop;
RX PubMed=229976; DOI=10.1016/0092-8674(79)90209-5;
RA Seif I., Khoury G., Dhar R.;
RT "The genome of human papovavirus BKV.";
RL Cell 18:963-977(1979).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MM;
RX PubMed=228391; DOI=10.1126/science.228391;
RA Yang R.C.A., Wu R.;
RT "BK virus DNA: complete nucleotide sequence of a human tumor virus.";
RL Science 206:456-462(1979).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RC STRAIN=MM;
RX PubMed=228246; DOI=10.1093/nar/7.3.651;
RA Yang R.C.A., Wu R.;
RT "Comparative study of papovavirus DNA: BKV(MM), BKV(WT) and SV40.";
RL Nucleic Acids Res. 7:651-668(1979).
RN [4]
RP IDENTIFICATION OF PROTEIN.
RX PubMed=6262654; DOI=10.1038/291346a0;
RA Jay G., Nomura S., Anderson C.W., Khoury G.;
RT "Identification of the SV40 agnogene product: a DNA binding protein.";
RL Nature 291:346-349(1981).
CC -!- FUNCTION: Alters the structure of the nuclear envelope by interacting
CC with host CBX5 and disrupting CBX5 association with LBR. Involved in
CC the perinuclear-nuclear localization of the capsid protein VP1 during
CC virion assembly and maturation. Plays an important role in the release
CC of progeny virions from infected cells and in viral propagation,
CC probably by acting as a viral ionic channel in the host plasma
CC membrane. Allows influx of extracellular calcium ions in the host cell.
CC May contribute to viral genome transcription and translation of viral
CC late proteins (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homooligomer. Interacts with VP1 (By similarity). Interacts
CC with large T antigen; this interaction may impact upon the activity of
CC T-antigen on the control of viral gene transcription and replication.
CC Interacts with small t antigen. Interacts with host CBX5; this
CC interaction induces the dissociation of CBX5 from LBR, resulting in
CC destabilization of the nuclear envelope (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}. Host nucleus
CC membrane {ECO:0000305}; Single-pass type II membrane protein
CC {ECO:0000305}. Host rough endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}. Host cell membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC Note=Mostly perinuclear. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing, Alternative initiation; Named isoforms=5;
CC Name=Agno;
CC IsoId=P03085-1; Sequence=Displayed;
CC Name=VP1; Synonyms=Major capsid protein VP1;
CC IsoId=P03088-1; Sequence=External;
CC Name=VP2; Synonyms=Minor capsid protein VP2;
CC IsoId=P03094-1; Sequence=External;
CC Name=VP3; Synonyms=Minor capsid protein VP3;
CC IsoId=P03094-2; Sequence=External;
CC Name=VP4; Synonyms=Viroporin VP4;
CC IsoId=P03094-3; Sequence=External;
CC -!- PTM: Phosphorylated by host PKC. Phosphorylation alters the stability
CC and may also have an impact on the subcellular location.
CC -!- MISCELLANEOUS: [Isoform Agno]: Produced by alternative initiation of
CC the late mRNA.
CC -!- SIMILARITY: Belongs to the polyomavirus agnoprotein family.
CC {ECO:0000305}.
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DR EMBL; V01108; CAA24296.1; -; Genomic_DNA.
DR EMBL; V01109; CAA24304.1; -; Genomic_DNA.
DR EMBL; M57959; AAA47146.2; -; Genomic_DNA.
DR EMBL; D00678; BAA00584.1; -; Genomic_DNA.
DR PIR; B03632; DNVPB.
DR RefSeq; YP_717936.1; NC_001538.1. [P03085-1]
DR SMR; P03085; -.
DR PRIDE; P03085; -.
DR DNASU; 29031013; -.
DR GeneID; 29031013; -.
DR KEGG; vg:29031013; -.
DR Proteomes; UP000008475; Genome.
DR Proteomes; UP000008990; Genome.
DR GO; GO:0044200; C:host cell nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044169; C:host cell rough endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0005216; F:ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW.
DR GO; GO:0051259; P:protein complex oligomerization; IEA:UniProtKB-KW.
DR InterPro; IPR002643; Polyoma_agno.
DR Pfam; PF01736; Polyoma_agno; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Alternative splicing; Host cell membrane;
KW Host cytoplasm; Host endoplasmic reticulum; Host membrane; Host nucleus;
KW Host-virus interaction; Ion channel; Ion transport; Membrane;
KW Phosphoprotein; Signal-anchor; Transmembrane; Transmembrane helix;
KW Transport; Viral ion channel.
FT CHAIN 1..66
FT /note="Agnoprotein"
FT /id="PRO_0000115031"
FT TOPO_DOM 1..27
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 28..44
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..66
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT MOD_RES 7
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT MOD_RES 11
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT MOD_RES 21
FT /note="Phosphothreonine; by host"
FT /evidence="ECO:0000250"
SQ SEQUENCE 66 AA; 7397 MW; 0198F846AC787EA9 CRC64;
MVLRQLSRQA SVKVGKTWTG TKKRAQRIFI FILELLLEFC RGEDSVDGKN KSTTALPAVK
DSVKDS
