ENY2_ARATH
ID ENY2_ARATH Reviewed; 115 AA.
AC Q6NQ54;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Transcription and mRNA export factor ENY2 {ECO:0000255|HAMAP-Rule:MF_03046};
DE AltName: Full=Enhancer of yellow 2 transcription factor homolog {ECO:0000255|HAMAP-Rule:MF_03046};
GN Name=ENY2 {ECO:0000303|PubMed:29588169};
GN OrderedLocusNames=At3g27100 {ECO:0000312|Araport:AT3G27100};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION.
RX PubMed=19843313; DOI=10.1111/j.1365-313x.2009.04048.x;
RA Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E.,
RA Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y.;
RT "Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components
RT and anchoring nucleoporin.";
RL Plant J. 61:259-270(2010).
RN [6]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, INTERACTION WITH
RP SGF11 AND MOS4, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=29588169; DOI=10.1016/j.jmb.2018.03.018;
RA Pfab A., Bruckmann A., Nazet J., Merkl R., Grasser K.D.;
RT "The adaptor protein ENY2 is a component of the deubiquitination module of
RT the Arabidopsis SAGA transcriptional co-activator complex but not of the
RT TREX-2 complex.";
RL J. Mol. Biol. 430:1479-1494(2018).
RN [7]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, INTERACTION WITH
RP SGF11, AND SUBCELLULAR LOCATION.
RX PubMed=30192741; DOI=10.7554/elife.37892;
RA Nassrallah A., Rougee M., Bourbousse C., Drevensek S., Fonseca S.,
RA Iniesto E., Ait-Mohamed O., Deton-Cabanillas A.F., Zabulon G., Ahmed I.,
RA Stroebel D., Masson V., Lombard B., Eeckhout D., Gevaert K., Loew D.,
RA Genovesio A., Breyton C., de Jaeger G., Bowler C., Rubio V., Barneche F.;
RT "DET1-mediated degradation of a SAGA-like deubiquitination module controls
RT H2Bub homeostasis.";
RL Elife 7:0-0(2018).
CC -!- FUNCTION: Component of a deubiquitination module (DUB module) that
CC specifically deubiquinates monoubiquinated histone H2B (H2Bub)
CC (PubMed:29588169, PubMed:30192741). Does not seem to be a component of
CC the TREX-2 complex (PubMed:29588169). Seems to act independently of the
CC SAGA multiprotein complex (PubMed:30192741). The DUB module is
CC responsible for the major H2Bub deubiquitinase activity in Arabidopsis
CC (PubMed:30192741). {ECO:0000269|PubMed:29588169,
CC ECO:0000269|PubMed:30192741}.
CC -!- SUBUNIT: Component of a deubiquitination module (DUB module) formed by
CC ENY2, SGF11, and UBP22 in Arabidopsis (PubMed:29588169,
CC PubMed:30192741). Interacts directly with SGF11, but not with UBP22
CC (PubMed:29588169, PubMed:30192741). Interacts with MOS4
CC (PubMed:29588169). {ECO:0000269|PubMed:29588169,
CC ECO:0000269|PubMed:30192741}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000255|HAMAP-
CC Rule:MF_03046, ECO:0000269|PubMed:29588169,
CC ECO:0000269|PubMed:30192741}. Note=Does not localize in nucleolus and
CC displays a rather patchy distribution forming a speckled pattern in the
CC nucleoplasm (PubMed:29588169, PubMed:30192741). Does not localize in
CC the nuclear pore complex at the nuclear periphery (PubMed:29588169,
CC PubMed:30192741). {ECO:0000269|PubMed:29588169,
CC ECO:0000269|PubMed:30192741}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, cotyledons, leaves and upper
CC part of sepals. {ECO:0000269|PubMed:29588169}.
CC -!- SIMILARITY: Belongs to the ENY2 family. {ECO:0000255|HAMAP-
CC Rule:MF_03046}.
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DR EMBL; AB026649; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002686; AEE77266.1; -; Genomic_DNA.
DR EMBL; BT010609; AAQ89631.1; -; mRNA.
DR EMBL; AK175619; BAD43382.1; -; mRNA.
DR RefSeq; NP_189346.2; NM_113624.5.
DR AlphaFoldDB; Q6NQ54; -.
DR SMR; Q6NQ54; -.
DR BioGRID; 7659; 1.
DR IntAct; Q6NQ54; 1.
DR STRING; 3702.AT3G27100.2; -.
DR iPTMnet; Q6NQ54; -.
DR PRIDE; Q6NQ54; -.
DR ProteomicsDB; 221944; -.
DR EnsemblPlants; AT3G27100.1; AT3G27100.1; AT3G27100.
DR GeneID; 822329; -.
DR Gramene; AT3G27100.1; AT3G27100.1; AT3G27100.
DR KEGG; ath:AT3G27100; -.
DR Araport; AT3G27100; -.
DR eggNOG; KOG4479; Eukaryota.
DR HOGENOM; CLU_134052_0_1_1; -.
DR OMA; RLMCRNI; -.
DR OrthoDB; 1538551at2759; -.
DR PhylomeDB; Q6NQ54; -.
DR PRO; PR:Q6NQ54; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q6NQ54; baseline and differential.
DR Genevisible; Q6NQ54; AT.
DR GO; GO:0071819; C:DUBm complex; IBA:GO_Central.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-UniRule.
DR GO; GO:0005654; C:nucleoplasm; IDA:CACAO.
DR GO; GO:0000124; C:SAGA complex; IBA:GO_Central.
DR GO; GO:0070390; C:transcription export complex 2; IEA:UniProtKB-UniRule.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0016578; P:histone deubiquitination; IBA:GO_Central.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.246.140; -; 1.
DR HAMAP; MF_03046; ENY2_Sus1; 1.
DR InterPro; IPR018783; TF_ENY2.
DR InterPro; IPR038212; TF_EnY2_sf.
DR PANTHER; PTHR12514; PTHR12514; 1.
DR Pfam; PF10163; EnY2; 1.
PE 1: Evidence at protein level;
KW Activator; Chromatin regulator; mRNA transport; Nucleus; Protein transport;
KW Reference proteome; Transcription; Transcription regulation; Translocation;
KW Transport; Ubl conjugation pathway.
FT CHAIN 1..115
FT /note="Transcription and mRNA export factor ENY2"
FT /id="PRO_0000423831"
SQ SEQUENCE 115 AA; 13107 MW; DCA3CE63CFC4E026 CRC64;
MKHSVNRPPT PDEDDVADGF EKDKVTLREI INVKLVESGE KENLMELVRD RLVECGWKDE
MRIACREHVK KKGRKDVTVD ELIRVITPKG RASVPDSVKA ELLNRIQNFI VSAAL
