EOBII_PETHY
ID EOBII_PETHY Reviewed; 197 AA.
AC E0CJS3; F8S2G9;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=MYB-like transcription factor EOBII {ECO:0000303|PubMed:20543029};
DE AltName: Full=MYB-like protein NON1 {ECO:0000303|PubMed:21464473};
DE Short=PhNON1 {ECO:0000303|PubMed:21464473};
DE AltName: Full=Protein EMISSION OF BENZENOIDS II {ECO:0000303|PubMed:20543029};
DE Short=PhEOBII {ECO:0000303|PubMed:21464473};
GN Name=EOBII {ECO:0000303|PubMed:20543029};
GN Synonyms=NON1 {ECO:0000303|PubMed:21464473};
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Mitchell;
RA Verdonk J.C., Colquhoun T.A., Kim J.Y., Underwood B.A., Clark D.G.;
RT "Differential expression of transcription factors in petunia flowers.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL
RP STAGE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Violet 26;
RX PubMed=20543029; DOI=10.1105/tpc.109.067280;
RA Spitzer-Rimon B., Marhevka E., Barkai O., Marton I., Edelbaum O., Masci T.,
RA Prathapani N.K., Shklarman E., Ovadis M., Vainstein A.;
RT "EOBII, a gene encoding a flower-specific regulator of phenylpropanoid
RT volatiles' biosynthesis in petunia.";
RL Plant Cell 22:1961-1976(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9, FUNCTION, DISRUPTION PHENOTYPE,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Mitchell;
RX PubMed=21464473; DOI=10.1104/pp.111.176248;
RA Colquhoun T.A., Schwieterman M.L., Wedde A.E., Schimmel B.C.J.,
RA Marciniak D.M., Verdonk J.C., Kim J.Y., Oh Y., Galis I., Baldwin I.T.,
RA Clark D.G.;
RT "EOBII controls flower opening by functioning as a general transcriptomic
RT switch.";
RL Plant Physiol. 156:974-984(2011).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Mitchell, cv. R27, and cv. Violet 26;
RX PubMed=21585571; DOI=10.1111/j.1365-313x.2011.04644.x;
RA Van Moerkercke A., Haring M.A., Schuurink R.C.;
RT "The transcription factor EMISSION OF BENZENOIDS II activates the MYB
RT ODORANT1 promoter at a MYB binding site specific for fragrant petunias.";
RL Plant J. 67:917-928(2011).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. W115;
RX PubMed=23275577; DOI=10.1105/tpc.112.105247;
RA Spitzer-Rimon B., Farhi M., Albo B., Cna'ani A., Ben Zvi M.M., Masci T.,
RA Edelbaum O., Yu Y., Shklarman E., Ovadis M., Vainstein A.;
RT "The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII,
RT regulates scent production by activating ODO1 and structural scent-related
RT genes in petunia.";
RL Plant Cell 24:5089-5105(2012).
RN [6]
RP FUNCTION.
RC STRAIN=cv. Mitchell, and cv. R27;
RX PubMed=22499185; DOI=10.4161/psb.19311;
RA Van Moerkercke A., Haring M.A., Schuurink R.C.;
RT "A model for combinatorial regulation of the petunia R2R3-MYB transcription
RT factor ODORANT1.";
RL Plant Signal. Behav. 7:518-520(2012).
CC -!- FUNCTION: MYB-type transcription factor controlling the production of
CC volatile organic compounds (VOCs), including floral volatile benzenoids
CC and phenylpropanoids (FVBP), in flowers of fragrant cultivars (e.g. cv.
CC Mitchell and cv. V26) by regulating the expression of ODO1 and EOBI,
CC key regulators of the shikimate pathway, and of several biosynthetic
CC floral scent-related genes including IGS, PAL2 and CFAT
CC (PubMed:21585571, PubMed:20543029, PubMed:23275577). This scent, mostly
CC produced in the evening and night by the petals, attracts the
CC pollinators (e.g. the night-active hawkmoth pollinator Manduca sexta)
CC (PubMed:21585571, PubMed:20543029). Binds to and activates the ODO1 and
CC EOBI promoters via MYB binding sites (MBS) 5'-AAACCTAAT-3' and 5'-
CC CTAACT-3' (PubMed:21585571, PubMed:22499185, PubMed:20543029,
CC PubMed:23275577). Regulates the promoters of IGS1, CFAT and PAL2
CC (PubMed:20543029). Controls flowers petal opening by modulating a
CC global transcriptomic switch (PubMed:21464473).
CC {ECO:0000269|PubMed:20543029, ECO:0000269|PubMed:21464473,
CC ECO:0000269|PubMed:21585571, ECO:0000269|PubMed:22499185,
CC ECO:0000269|PubMed:23275577}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:20543029}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in flowers, mostly in
CC stigmas, petal tubes and petal limbs, and, to a lower extent, in
CC anthers and stamen (PubMed:21585571, PubMed:20543029, PubMed:21464473).
CC Also present at low levels in roots, stems, leaves and sepals
CC (PubMed:20543029, PubMed:21464473). {ECO:0000269|PubMed:20543029,
CC ECO:0000269|PubMed:21464473, ECO:0000269|PubMed:21585571}.
CC -!- DEVELOPMENTAL STAGE: Expressed early in flower development
CC (PubMed:20543029). In corollas, accumulates progressively during flower
CC development, from buds to anthesis, with a peak at flower opening, but
CC fades out in senescing flowers (PubMed:20543029, PubMed:21464473).
CC {ECO:0000269|PubMed:20543029, ECO:0000269|PubMed:21464473}.
CC -!- INDUCTION: Circadian-regulation with peak levels occurring in the early
CC morning hours and lowest levels during the late afternoon hours in
CC flowers. {ECO:0000269|PubMed:20543029}.
CC -!- DISRUPTION PHENOTYPE: Impaired expression of ODO1, EOBI and several
CC biosynthetic floral scent-related genes (e.g. IGS, PAL2 and CFAT)
CC leading to reduced scent production in flowers (e.g. phenylpropanoid
CC volatiles including benzaldehyde, phenylethyl alcohol, benzylbenzoate
CC and isoeugenol) (PubMed:20543029, PubMed:21464473, PubMed:23275577).
CC Impaired anthesis enter and premature senescence of flowers
CC (PubMed:21464473). Altered cell wall modifier transcript levels in
CC flowers (PubMed:21464473). {ECO:0000269|PubMed:20543029,
CC ECO:0000269|PubMed:21464473, ECO:0000269|PubMed:23275577}.
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DR EMBL; EU374207; ACB12237.1; -; mRNA.
DR EMBL; EU360893; ACB59077.1; -; mRNA.
DR EMBL; HQ450382; AEI83419.1; -; Genomic_DNA.
DR AlphaFoldDB; E0CJS3; -.
DR SMR; E0CJS3; -.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009505; C:plant-type cell wall; IMP:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0080187; P:floral organ senescence; IMP:UniProtKB.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:1903338; P:regulation of cell wall organization or biogenesis; IMP:UniProtKB.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IDA:UniProtKB.
DR GO; GO:2000762; P:regulation of phenylpropanoid metabolic process; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR044676; EOBI/EOBII-like_plant.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR PANTHER; PTHR45675; PTHR45675; 1.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Flowering; Nucleus; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..197
FT /note="MYB-like transcription factor EOBII"
FT /id="PRO_0000451491"
FT DOMAIN 10..62
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 63..117
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 38..62
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 90..113
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 125..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 197 AA; 22585 MW; 4664A708893EED30 CRC64;
MDKKPCNSQD AEVRKGPWTM EEDLILINYI ANHGEGVWNS LAKSAGLKRT GKSCRLRWLN
YLRPDVRRGN ITPEEQLLIM ELHAKWGNRW SKIAKHLPGR TDNEIKNYWR TRIQKHIKQA
ETMNGQAASS EQNDHQEACT SQMSNGPNDN TIDQTYSPTS YSGNVDTFQA GPNFLTEAND
NMWSMEDIWS MQLLNGD
