EOBI_PETHY
ID EOBI_PETHY Reviewed; 201 AA.
AC L7R9Z0; F1LKD1;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=MYB-like transcription factor EOBI {ECO:0000303|PubMed:23275577};
DE AltName: Full=Protein EMISSION OF BENZENOIDS I {ECO:0000303|PubMed:23275577};
DE Short=PhEOBI {ECO:0000303|PubMed:23275577};
GN Name=EOBI {ECO:0000303|PubMed:23275577};
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY
RP EOBII, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, SUBCELLULAR
RP LOCATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. W115;
RX PubMed=23275577; DOI=10.1105/tpc.112.105247;
RA Spitzer-Rimon B., Farhi M., Albo B., Cna'ani A., Ben Zvi M.M., Masci T.,
RA Edelbaum O., Yu Y., Shklarman E., Ovadis M., Vainstein A.;
RT "The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII,
RT regulates scent production by activating ODO1 and structural scent-related
RT genes in petunia.";
RL Plant Cell 24:5089-5105(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 12-116.
RC STRAIN=cv. Violet 26;
RA Spitzer B., Marhevka E., Ovadis M., Barkai O., Vainstein A.;
RT "EOBI phenylpropanoid pathway-related transcription factor.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: MYB-type transcription factor controlling the production of
CC volatile organic compounds (VOCs), including floral volatile benzenoids
CC and phenylpropanoids (FVBP), in flowers of fragrant cultivars (e.g. cv.
CC Mitchell and cv. V26) by regulating the expression of ODO1, a key
CC regulator of the shikimate pathway, and of several biosynthetic floral
CC scent-related genes (e.g. IGS, EGS, BSMT1, BSMT2, PAL1, PAL2, EPSPS,
CC DAHPS, CS, CM1, ADT1 and PPA-AT) (PubMed:23275577). Binds to and
CC activates the promoters of at least ODO1, IGS1 and PAL1
CC (PubMed:23275577). {ECO:0000269|PubMed:23275577}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:23275577}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in flower organs
CC (PubMed:23275577). Accumulates mostly in flower limbs, to a lower
CC extent in pistils and flower tubes, and, at low levels, in stamens
CC (PubMed:23275577). {ECO:0000269|PubMed:23275577}.
CC -!- DEVELOPMENTAL STAGE: Accumulates progressively in flower limbs during
CC flower development, reaching maximal levels at anthesis.
CC {ECO:0000269|PubMed:23275577}.
CC -!- INDUCTION: Circadian-regulation with higher levels during the light
CC period in flowers (PubMed:23275577). Activated by EOBII but repressed
CC by ODO1 via the regulation of its promoter (PubMed:23275577).
CC {ECO:0000269|PubMed:23275577}.
CC -!- DISRUPTION PHENOTYPE: Down-regulation of ODO1 and numerous structural
CC scent-related genes from both the shikimate and phenylpropanoid
CC pathways (e.g. IGS, EGS, BSMT1, BSMT2, PAL1, PAL2, EPSPS, DAHPS, CS,
CC CM1, ADT1 and PPA-AT) leading to reduced volatiles scent production
CC (e.g. phenylpropanoid: benzyl alcohol, benzylbenzoate, methylbenzoate,
CC methylsalicylate, eugenol and isoeugenol).
CC {ECO:0000269|PubMed:23275577}.
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DR EMBL; KC182627; AGC00813.1; -; mRNA.
DR EMBL; EU360892; ACB59076.2; -; mRNA.
DR AlphaFoldDB; L7R9Z0; -.
DR SMR; L7R9Z0; -.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:2000762; P:regulation of phenylpropanoid metabolic process; IMP:UniProtKB.
DR GO; GO:0019632; P:shikimate metabolic process; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR044676; EOBI/EOBII-like_plant.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR PANTHER; PTHR45675; PTHR45675; 1.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..201
FT /note="MYB-like transcription factor EOBI"
FT /id="PRO_0000451490"
FT DOMAIN 10..62
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 63..117
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 38..62
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 90..113
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 121..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 201 AA; 23315 MW; 14AA0A907B0A2F9E CRC64;
MDKRTCNSQD VEVRKGPWTM EEDLILINYI ANHGEGVWNS LARSAGLKRT GKSCRLRWLN
YLRPDVRRGN ITPEEQLLIM ELHAKWGNRW SKIAKHLPGR TDNEIKNYWR TRIQKHIKQA
DQNMKKPSKC EQNDQKAIST SQASTGPTDT IDSYSPSSYT ENTNNNMENI TFQGNFPTET
NENIWSMEDL WSLQLLNDAT N
