EPA2B_XENLA
ID EPA2B_XENLA Reviewed; 218 AA.
AC Q6TY21;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Embryonic polyadenylate-binding protein 2-B;
DE Short=Embryonic poly(A)-binding protein 2-B;
DE Short=XePABP2-B;
DE Short=ePABP-2B;
DE Short=ePABP2-B;
DE AltName: Full=Embryonic poly(A)-binding protein type II-B;
GN Name=Pabpn1l-b; Synonyms=epabp2-b, pabpnl1-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAR26263.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Oocyte {ECO:0000269|PubMed:15083517};
RX PubMed=15083517; DOI=10.1002/gene.20015;
RA Good P.J., Abler L., Herring D., Sheets M.D.;
RT "Xenopus embryonic poly(A) binding protein 2 (ePABP2) defines a new family
RT of cytoplasmic poly(A) binding proteins expressed during the early stages
RT of vertebrate development.";
RL Genesis 38:166-175(2004).
RN [2]
RP STRUCTURE BY NMR OF 60-180, AND SUBUNIT.
RX PubMed=18824697; DOI=10.1073/pnas.0801274105;
RA Song J., McGivern J.V., Nichols K.W., Markley J.L., Sheets M.D.;
RT "Structural basis for RNA recognition by a type II poly(A)-binding
RT protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:15317-15322(2008).
CC -!- FUNCTION: Binds the poly(A) tail of mRNA. Unable to interact with the
CC cap-binding complex and is therefore unlikely to be involved in
CC translation initiation. {ECO:0000250|UniProtKB:Q804A5,
CC ECO:0000269|PubMed:15083517}.
CC -!- SUBUNIT: Homodimer; Upon poly(A) binding, undergoes a dimer-monomer
CC transition that removes the polyproline motif from the RNA recognition
CC site and allows it to be replaced by the adenosine nucleotides of
CC poly(A). {ECO:0000269|PubMed:18824697}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15083517}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Restricted to oogenesis, early embryogenesis and the adult ovary.
CC Levels decrease after the onset of zygotic transcription (at protein
CC level). {ECO:0000269|PubMed:15083517}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY382837; AAR26263.1; -; mRNA.
DR RefSeq; NP_001084418.1; NM_001090949.1.
DR PDB; 2JWN; NMR; -; A/B=60-180.
DR PDBsum; 2JWN; -.
DR AlphaFoldDB; Q6TY21; -.
DR BMRB; Q6TY21; -.
DR SMR; Q6TY21; -.
DR GeneID; 403372; -.
DR KEGG; xla:403372; -.
DR CTD; 403372; -.
DR Xenbase; XB-GENE-1000606; pabpn1l.S.
DR OrthoDB; 1412946at2759; -.
DR EvolutionaryTrace; Q6TY21; -.
DR Proteomes; UP000186698; Chromosome 4S.
DR Bgee; 403372; Expressed in oocyte and 6 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0008143; F:poly(A) binding; IDA:UniProtKB.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; RNA-binding.
FT CHAIN 1..218
FT /note="Embryonic polyadenylate-binding protein 2-B"
FT /id="PRO_0000239464"
FT DOMAIN 93..170
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 84..92
FT /evidence="ECO:0007829|PDB:2JWN"
FT STRAND 94..100
FT /evidence="ECO:0007829|PDB:2JWN"
FT HELIX 106..114
FT /evidence="ECO:0007829|PDB:2JWN"
FT STRAND 119..127
FT /evidence="ECO:0007829|PDB:2JWN"
FT STRAND 134..143
FT /evidence="ECO:0007829|PDB:2JWN"
FT HELIX 144..151
FT /evidence="ECO:0007829|PDB:2JWN"
FT TURN 152..155
FT /evidence="ECO:0007829|PDB:2JWN"
FT STRAND 164..169
FT /evidence="ECO:0007829|PDB:2JWN"
SQ SEQUENCE 218 AA; 24270 MW; 3571AC009E95EDBA CRC64;
MSERVSEEPG LDKGDRAEEC ELDDPELKAI RMRVREMEEE AERLKGLSGQ DKSIGVSTRP
CMQTTHSKMT AGAYTEGPPQ PLSAEEKKEI DKRSVYVGNV DYGSTAQDLE AHFSSCGSIN
RITILCDKFS GHPKGYAYIE FAERNSVDAA VAMDETVFRG RTIKVLPKRT NMPGISSTDR
GGFRGRPRGN RGNYQRGQRP RGRPFRGRGR PGPLNNPY
