ID   EPFL1_ARATH             Reviewed;         122 AA.
AC   Q9LFT5;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=EPIDERMAL PATTERNING FACTOR-like protein 1 {ECO:0000303|PubMed:19435754};
DE            Short=EPF-like protein 1;
DE   Contains:
DE     RecName: Full=MEPFL1 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=EPFL1 {ECO:0000303|PubMed:19435754};
GN   OrderedLocusNames=At5g10310 {ECO:0000312|Araport:AT5G10310};
GN   ORFNames=F18D22.80;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19435754; DOI=10.1093/pcp/pcp068;
RA   Hara K., Yokoo T., Kajita R., Onishi T., Yahata S., Peterson K.M.,
RA   Torii K.U., Kakimoto T.;
RT   "Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL
RT   PATTERNING FACTOR 2 in Arabidopsis leaves.";
RL   Plant Cell Physiol. 50:1019-1031(2009).
RN   [5]
RP   3D-STRUCTURE MODELING, AND DISULFIDE BOND.
RX   PubMed=22027592; DOI=10.1038/ncomms1520;
RA   Ohki S., Takeuchi M., Mori M.;
RT   "The NMR structure of stomagen reveals the basis of stomatal density
RT   regulation by plant peptide hormones.";
RL   Nat. Commun. 2:512-512(2011).
CC   -!- FUNCTION: Controls stomatal patterning. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant cysteine rich small secretory peptide
CC       family. Epidermal patterning factor subfamily. {ECO:0000305}.
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DR   EMBL; AL360334; CAB96687.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED91519.1; -; Genomic_DNA.
DR   EMBL; BT010810; AAR24177.1; -; mRNA.
DR   EMBL; BT011282; AAR92318.1; -; mRNA.
DR   PIR; T50819; T50819.
DR   RefSeq; NP_196593.1; NM_121069.4.
DR   AlphaFoldDB; Q9LFT5; -.
DR   SMR; Q9LFT5; -.
DR   STRING; 3702.AT5G10310.1; -.
DR   PaxDb; Q9LFT5; -.
DR   PRIDE; Q9LFT5; -.
DR   EnsemblPlants; AT5G10310.1; AT5G10310.1; AT5G10310.
DR   GeneID; 830895; -.
DR   Gramene; AT5G10310.1; AT5G10310.1; AT5G10310.
DR   KEGG; ath:AT5G10310; -.
DR   Araport; AT5G10310; -.
DR   TAIR; locus:2145367; AT5G10310.
DR   eggNOG; ENOG502SAGJ; Eukaryota.
DR   HOGENOM; CLU_135272_2_0_1; -.
DR   InParanoid; Q9LFT5; -.
DR   OMA; NSKINMF; -.
DR   PhylomeDB; Q9LFT5; -.
DR   PRO; PR:Q9LFT5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LFT5; baseline and differential.
DR   Genevisible; Q9LFT5; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0010052; P:guard cell differentiation; ISS:UniProtKB.
DR   GO; GO:0010374; P:stomatal complex development; ISS:UniProtKB.
DR   InterPro; IPR039455; EPFL.
DR   PANTHER; PTHR33109; PTHR33109; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Disulfide bond; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..122
FT                   /note="EPIDERMAL PATTERNING FACTOR-like protein 1"
FT                   /id="PRO_0000392499"
FT   CHAIN           48..122
FT                   /note="MEPFL1"
FT                   /evidence="ECO:0000303|PubMed:22027592"
FT                   /id="PRO_0000430507"
FT   DISULFID        55..113
FT                   /evidence="ECO:0000303|PubMed:22027592"
FT   DISULFID        59..65
FT                   /evidence="ECO:0000303|PubMed:22027592"
FT   DISULFID        62..115
FT                   /evidence="ECO:0000303|PubMed:22027592"
SQ   SEQUENCE   122 AA;  13652 MW;  92C807991AB38D52 CRC64;
     MFAIYKSTLL LLPLILILLI TPQVSSFLQP IQPPISPQVA LIEDKARLGS TPPSCHNRCN
     NCHPCMAIQV PTLPTRSRFT RVNPFSGGFV RPPSSLTTVL DQYSNYKPMG WKCHCNGHFY
     NP