ID   EPFL2_ARATH             Reviewed;         128 AA.
AC   Q9T068;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=EPIDERMAL PATTERNING FACTOR-like protein 2 {ECO:0000303|PubMed:19435754};
DE            Short=EPF-like protein 2;
DE   Contains:
DE     RecName: Full=MEPFL2 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=EPFL2 {ECO:0000303|PubMed:19435754};
GN   OrderedLocusNames=At4g37810 {ECO:0000312|Araport:AT4G37810};
GN   ORFNames=T28I19.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19435754; DOI=10.1093/pcp/pcp068;
RA   Hara K., Yokoo T., Kajita R., Onishi T., Yahata S., Peterson K.M.,
RA   Torii K.U., Kakimoto T.;
RT   "Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL
RT   PATTERNING FACTOR 2 in Arabidopsis leaves.";
RL   Plant Cell Physiol. 50:1019-1031(2009).
RN   [4]
RP   3D-STRUCTURE MODELING, AND DISULFIDE BOND.
RX   PubMed=22027592; DOI=10.1038/ncomms1520;
RA   Ohki S., Takeuchi M., Mori M.;
RT   "The NMR structure of stomagen reveals the basis of stomatal density
RT   regulation by plant peptide hormones.";
RL   Nat. Commun. 2:512-512(2011).
CC   -!- FUNCTION: Controls stomatal patterning. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant cysteine rich small secretory peptide
CC       family. Epidermal patterning factor subfamily. {ECO:0000305}.
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DR   EMBL; AL035709; CAB38929.1; -; Genomic_DNA.
DR   EMBL; AL161592; CAB80446.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE86840.1; -; Genomic_DNA.
DR   PIR; T06028; T06028.
DR   RefSeq; NP_680774.1; NM_148408.3.
DR   AlphaFoldDB; Q9T068; -.
DR   SMR; Q9T068; -.
DR   STRING; 3702.AT4G37810.1; -.
DR   PaxDb; Q9T068; -.
DR   PRIDE; Q9T068; -.
DR   EnsemblPlants; AT4G37810.1; AT4G37810.1; AT4G37810.
DR   GeneID; 829937; -.
DR   Gramene; AT4G37810.1; AT4G37810.1; AT4G37810.
DR   KEGG; ath:AT4G37810; -.
DR   Araport; AT4G37810; -.
DR   TAIR; locus:2137614; AT4G37810.
DR   eggNOG; ENOG502S846; Eukaryota.
DR   HOGENOM; CLU_135272_0_0_1; -.
DR   InParanoid; Q9T068; -.
DR   OMA; TQLRYKA; -.
DR   OrthoDB; 1567319at2759; -.
DR   PhylomeDB; Q9T068; -.
DR   PRO; PR:Q9T068; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9T068; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0010052; P:guard cell differentiation; ISS:UniProtKB.
DR   GO; GO:0010374; P:stomatal complex development; ISS:UniProtKB.
DR   InterPro; IPR039455; EPFL.
DR   PANTHER; PTHR33109; PTHR33109; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Disulfide bond; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..128
FT                   /note="EPIDERMAL PATTERNING FACTOR-like protein 2"
FT                   /id="PRO_0000392500"
FT   CHAIN           53..128
FT                   /note="MEPFL2"
FT                   /evidence="ECO:0000303|PubMed:22027592"
FT                   /id="PRO_0000430508"
FT   REGION          79..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        60..119
FT                   /evidence="ECO:0000303|PubMed:22027592"
FT   DISULFID        65..71
FT                   /evidence="ECO:0000303|PubMed:22027592"
FT   DISULFID        68..121
FT                   /evidence="ECO:0000303|PubMed:22027592"
SQ   SEQUENCE   128 AA;  14318 MW;  DE5E08A319E1B36C CRC64;
     MVWSSNMSSF LLILLILNST HFSLMANGRP EPDSVEFTKS GDQDVKMMMR GLIGSRPPRC
     ERVRCRSCGH CEAIQVPTNP QTKLHSPLTT SSSSSSETIH LDYTRGDDST NYKPMSWKCK
     CGNSIYNP