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EPG2_CAEEL
ID   EPG2_CAEEL              Reviewed;         690 AA.
AC   Q95XR4;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Ectopic P granules protein 2;
GN   Name=epg-2 {ECO:0000312|WormBase:Y39G10AR.10};
GN   ORFNames=Y39G10AR.10 {ECO:0000312|WormBase:Y39G10AR.10};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=20550938; DOI=10.1016/j.cell.2010.04.034;
RA   Tian Y., Li Z., Hu W., Ren H., Tian E., Zhao Y., Lu Q., Huang X., Yang P.,
RA   Li X., Wang X., Kovacs A.L., Yu L., Zhang H.;
RT   "C. elegans screen identifies autophagy genes specific to multicellular
RT   organisms.";
RL   Cell 141:1042-1055(2010).
RN   [3]
RP   FUNCTION, INTERACTION WITH SEPA-1, SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=24140420; DOI=10.1016/j.molcel.2013.09.014;
RA   Li S., Yang P., Tian E., Zhang H.;
RT   "Arginine methylation modulates autophagic degradation of PGL granules in
RT   C. elegans.";
RL   Mol. Cell 52:421-433(2013).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24374177; DOI=10.1016/j.devcel.2013.11.022;
RA   Manil-Segalen M., Lefebvre C., Jenzer C., Trichet M., Boulogne C.,
RA   Satiat-Jeunemaitre B., Legouis R.;
RT   "The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by
RT   interacting with the HOPS subunit VPS39.";
RL   Dev. Cell 28:43-55(2014).
RN   [5]
RP   INTERACTION WITH LGG-1 AND LGG-2, DOMAIN LIR MOTIF, AND MUTAGENESIS OF
RP   59-LYS--LEU-64; 381-ASP--LEU-385; 683-GLU--VAL-687; 684-TRP--MET-686;
RP   TRP-684; GLU-685 AND VAL-687.
RX   PubMed=26687600; DOI=10.1016/j.molcel.2015.11.019;
RA   Wu F., Watanabe Y., Guo X.Y., Qi X., Wang P., Zhao H.Y., Wang Z.,
RA   Fujioka Y., Zhang H., Ren J.Q., Fang T.C., Shen Y.X., Feng W., Hu J.J.,
RA   Noda N.N., Zhang H.;
RT   "Structural Basis of the Differential Function of the Two C. elegans Atg8
RT   Homologs, LGG-1 and LGG-2, in Autophagy.";
RL   Mol. Cell 60:914-929(2015).
RN   [6]
RP   FUNCTION, INTERACTION WITH SEPA-1, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=28806108; DOI=10.1080/15548627.2017.1339843;
RA   Zhang G., Lin L., Qi D., Zhang H.;
RT   "The composition of a protein aggregate modulates the specificity and
RT   efficiency of its autophagic degradation.";
RL   Autophagy 13:1487-1495(2017).
CC   -!- FUNCTION: Involved in autophagy (PubMed:20550938, PubMed:24140420,
CC       PubMed:24374177). Thought to act as an adapter protein that brings PGL
CC       granules to autophagic structures containing lgg-1 (PubMed:20550938).
CC       Association with other adapters such as sepa-1 is required for the
CC       accumulation and degradation of germ cell specific P-granules by
CC       autophagy in somatic cells (PubMed:24140420, PubMed:28806108). This
CC       ensures exclusive localization of the P-granules in germ cells
CC       (PubMed:24140420, PubMed:28806108). May also play a role in the removal
CC       of sepa-1 from somatic cells (PubMed:28806108).
CC       {ECO:0000269|PubMed:20550938, ECO:0000269|PubMed:24140420,
CC       ECO:0000269|PubMed:24374177, ECO:0000269|PubMed:28806108}.
CC   -!- SUBUNIT: Interacts with sepa-1 (PubMed:24140420, PubMed:28806108).
CC       Interacts (via the LIR motifs) with lgg-1 and lgg-2 (PubMed:26687600).
CC       Shows strong interaction with lgg-1 and weak interaction with lgg-2
CC       (PubMed:26687600). {ECO:0000269|PubMed:24140420,
CC       ECO:0000269|PubMed:26687600, ECO:0000269|PubMed:28806108}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20550938,
CC       ECO:0000269|PubMed:24140420, ECO:0000269|PubMed:28806108}. Note=Co-
CC       localizes with sepa-1 in cytoplasmic aggregates.
CC       {ECO:0000269|PubMed:24140420}.
CC   -!- DEVELOPMENTAL STAGE: Aggregates of epg-2 peak in abundance at the 100
CC       cell stage then decrease until they are very few at the comma stage
CC       (PubMed:20550938, PubMed:28806108). Absent from the 200 stage onwards
CC       (PubMed:24140420, PubMed:28806108). {ECO:0000269|PubMed:20550938,
CC       ECO:0000269|PubMed:24140420, ECO:0000269|PubMed:28806108}.
CC   -!- DOMAIN: The LIR motifs (LC3-interacting region) are required for its
CC       interaction with lgg-1 and lgg-2. {ECO:0000269|PubMed:26687600}.
CC   -!- DISRUPTION PHENOTYPE: No colocalization of P-granule components with
CC       lgg-1 (PubMed:20550938). Furthermore, there is an accumulation of pgl-3
CC       positive P-granules in somatic cells of embryos (PubMed:28806108).
CC       RNAi-mediated knockdown results in increased lgg-2-positive
CC       autophagosomes following fertilization and at later embryonic stages
CC       (PubMed:24374177). {ECO:0000269|PubMed:20550938,
CC       ECO:0000269|PubMed:24374177, ECO:0000269|PubMed:28806108}.
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DR   EMBL; FO081209; CCD69908.1; -; Genomic_DNA.
DR   RefSeq; NP_490955.1; NM_058554.4.
DR   AlphaFoldDB; Q95XR4; -.
DR   SMR; Q95XR4; -.
DR   BioGRID; 37273; 8.
DR   DIP; DIP-27321N; -.
DR   IntAct; Q95XR4; 4.
DR   STRING; 6239.Y39G10AR.10; -.
DR   EPD; Q95XR4; -.
DR   PaxDb; Q95XR4; -.
DR   PeptideAtlas; Q95XR4; -.
DR   PRIDE; Q95XR4; -.
DR   EnsemblMetazoa; Y39G10AR.10.1; Y39G10AR.10.1; WBGene00021468.
DR   GeneID; 171787; -.
DR   KEGG; cel:CELE_Y39G10AR.10; -.
DR   UCSC; Y39G10AR.10; c. elegans.
DR   CTD; 171787; -.
DR   WormBase; Y39G10AR.10; CE26989; WBGene00021468; epg-2.
DR   eggNOG; ENOG502THXW; Eukaryota.
DR   HOGENOM; CLU_430982_0_0_1; -.
DR   InParanoid; Q95XR4; -.
DR   OMA; AINQEMI; -.
DR   OrthoDB; 1749076at2759; -.
DR   PhylomeDB; Q95XR4; -.
DR   PRO; PR:Q95XR4; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00021468; Expressed in embryo and 3 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR   GO; GO:0016236; P:macroautophagy; IMP:WormBase.
DR   GO; GO:1902902; P:negative regulation of autophagosome assembly; IMP:UniProtKB.
PE   1: Evidence at protein level;
KW   Autophagy; Coiled coil; Cytoplasm; Reference proteome.
FT   CHAIN           1..690
FT                   /note="Ectopic P granules protein 2"
FT                   /id="PRO_0000404707"
FT   REGION          381..385
FT                   /note="Required for interaction with lgg-1"
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   REGION          666..690
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          20..181
FT                   /evidence="ECO:0000255"
FT   COILED          359..409
FT                   /evidence="ECO:0000255"
FT   COILED          458..494
FT                   /evidence="ECO:0000255"
FT   COILED          560..643
FT                   /evidence="ECO:0000255"
FT   MOTIF           61..64
FT                   /note="LIR 1"
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MOTIF           684..687
FT                   /note="LIR 2"
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   COMPBIAS        666..681
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         59..64
FT                   /note="KTYSTL->ATASTA: Impairs the interaction with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         381..385
FT                   /note="DFKIL->KAKIA: Abolishes the interaction with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         683..687
FT                   /note="EWEMV->KAKMA: Impairs the interaction with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         684..686
FT                   /note="WEM->FVE: Impairs the interaction with lgg-1.
FT                   Abolishes the interaction with lgg-2."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         684
FT                   /note="W->A: Abolishes the interaction with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         684
FT                   /note="W->F: Abolishes the interaction with lgg-2, but not
FT                   with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         685
FT                   /note="E->K: Abolishes the interaction with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
FT   MUTAGEN         687
FT                   /note="V->A: Abolishes the interaction with lgg-1."
FT                   /evidence="ECO:0000269|PubMed:26687600"
SQ   SEQUENCE   690 AA;  79973 MW;  40730553023C5BAE CRC64;
     MSANRTVTVF SSSAEDQEPI ELAEDSLQNL DKMLAEEKEE HQLLKDEVVL LRKENVEAKT
     YSTLLEIMLD EAEEKASSAQ ETTSEENNLK ILNRDLVAEN LELKEMKEEL RKIWLSDSKK
     FQEALTRISD ENTKLQKDCH ELESIRQCAQ FALDNCNEEL EKTQTENEEH ESRIETLERE
     VCEKDIAMKD IVERKDEISL QLELQTKEFT SALNDLMYGR EDTLKQIHQM KENWKVKQNE
     FEVEITKLKS QNDYFDSERL QLTDRIRALL NELSDVRLEL GSTRLAMKEK AEVTEAVTSF
     NKDLRDKLED EIARLGECLQ FRKDEHEQDE AVIAHLEEQL KLGSDKAAAF SSEHSDTIEL
     LRESETELME LRMENYDLKE DFKILKEEKE DVNRTCECLR EQLSTTIQER DIEKGQMQSE
     MDAKMVAVHQ QYAKQIDNMK YNHMLAINQE LIKGQMALES GKKKHANEIL TVRNELEQSN
     AAHQSLRDQC SLLLSSEDDL RTAHLALESK MTLVSEECIA LRVSRANAQK EIGNLTEHHK
     LEVALLEDAK SGIQQRLHYA TIEIEQLKKI NEVTQAQFKK ETDEKNAEIN EFQAAMVSMK
     QQYNVLGNHC RVLTSQGISD RTTIDKLQET IREHTELAIE TKRIHDAEIV QLNDAHKKLV
     DNLGVEELDE EPKASTESEE KAEWEMVDEE
 
 
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