EPG5_MOUSE
ID EPG5_MOUSE Reviewed; 2572 AA.
AC Q80TA9;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Ectopic P granules protein 5 homolog;
GN Name=Epg5; Synonyms=Kiaa1632;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 996-2572.
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in autophagy. May play a role in a late step of
CC autophagy, such as clearance of autophagosomal cargo. Plays a key role
CC in innate and adaptive immune response triggered by unmethylated
CC cytidine-phosphate-guanosine (CpG) dinucleotides from pathogens, and
CC mediated by the nucleotide-sensing receptor TLR9. It is necessary for
CC the translocation of CpG dinucleotides from early endosomes to late
CC endosomes and lysosomes, where TLR9 is located.
CC {ECO:0000250|UniProtKB:Q9HCE0}.
CC -!- SUBUNIT: Interacts with RAN. {ECO:0000250|UniProtKB:Q9HCE0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q9HCE0}. Lysosome
CC {ECO:0000250|UniProtKB:Q9HCE0}.
CC -!- SIMILARITY: Belongs to the EPG5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC126553; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK122536; BAC65818.1; -; mRNA.
DR CCDS; CCDS57126.1; -.
DR RefSeq; NP_001182562.1; NM_001195633.1.
DR AlphaFoldDB; Q80TA9; -.
DR SMR; Q80TA9; -.
DR STRING; 10090.ENSMUSP00000038681; -.
DR iPTMnet; Q80TA9; -.
DR PhosphoSitePlus; Q80TA9; -.
DR EPD; Q80TA9; -.
DR MaxQB; Q80TA9; -.
DR PaxDb; Q80TA9; -.
DR PeptideAtlas; Q80TA9; -.
DR PRIDE; Q80TA9; -.
DR ProteomicsDB; 275751; -.
DR Antibodypedia; 22425; 80 antibodies from 15 providers.
DR Ensembl; ENSMUST00000044622; ENSMUSP00000038681; ENSMUSG00000039840.
DR GeneID; 100502841; -.
DR KEGG; mmu:100502841; -.
DR UCSC; uc008fsa.2; mouse.
DR CTD; 57724; -.
DR MGI; MGI:1918673; Epg5.
DR VEuPathDB; HostDB:ENSMUSG00000039840; -.
DR eggNOG; KOG3622; Eukaryota.
DR GeneTree; ENSGT00390000007354; -.
DR HOGENOM; CLU_000773_0_0_1; -.
DR InParanoid; Q80TA9; -.
DR OMA; EHFLPMY; -.
DR OrthoDB; 42984at2759; -.
DR PhylomeDB; Q80TA9; -.
DR TreeFam; TF313847; -.
DR BioGRID-ORCS; 100502841; 14 hits in 73 CRISPR screens.
DR ChiTaRS; Epg5; mouse.
DR PRO; PR:Q80TA9; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q80TA9; protein.
DR Bgee; ENSMUSG00000039840; Expressed in hindlimb stylopod muscle and 60 other tissues.
DR Genevisible; Q80TA9; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0097352; P:autophagosome maturation; IMP:MGI.
DR GO; GO:0006914; P:autophagy; IMP:MGI.
DR GO; GO:1990786; P:cellular response to dsDNA; ISS:UniProtKB.
DR GO; GO:0032456; P:endocytic recycling; IMP:MGI.
DR GO; GO:0008333; P:endosome to lysosome transport; ISS:UniProtKB.
DR GO; GO:0006862; P:nucleotide transport; ISS:UniProtKB.
DR GO; GO:0034162; P:toll-like receptor 9 signaling pathway; ISS:UniProtKB.
DR InterPro; IPR029651; EPG-5.
DR PANTHER; PTHR31139:SF4; PTHR31139:SF4; 1.
PE 1: Evidence at protein level;
KW Autophagy; Coiled coil; Cytoplasm; Lysosome; Reference proteome.
FT CHAIN 1..2572
FT /note="Ectopic P granules protein 5 homolog"
FT /id="PRO_0000306256"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 84..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1600..1626
FT /evidence="ECO:0000255"
FT COMPBIAS 48..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..123
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2572 AA; 290801 MW; 17637F25ACDB8482 CRC64;
MAEAVKPRRA KAKASRTKGK EKKKHEALQT CDAGPLPETC REQESPCPAS ELKGDDLKSS
ADPQLHSDVC GWNESEMFDI PLTSLTIGDE GPPVQDTEDL KERGEVTAGD GDDEMELKVD
PGDNVIAKGE PCKNFPEVED HTLIQCGPPE STLQPDFPCT QQAVEGSHAR EHPTRKQDEA
ALGCSKVFQN VSLHSSYEAK EVSQPPRVKK LYPELPAEIA EVPALVAVKP LLRSERLYPE
LPSQPEVTPF TKEQLKLLEP GSWLENVASY VEEFDNIAHQ DRHEFYELLL NYSRCRKQLL
LAEAELLTLM SDCHSAKSRL WHFKDEQMAV QGICADQVKV YGHHHYQRVE MNENVLGELK
KLFDAKSEHL HQTLTLHSYT SVLSRLQVES YIFTLLNSSA ALRSLAVYQA DQVPKLTESI
PSDVCQLKEC ISVLFMFTRR VSEDAQFHED ILLWLQKLVS VLQRVGCPGD HFFLLNHVLR
CPAGIRKWAV PFIQIKVLNN PSGVFHFMQS LALLMSPVKN RAEFMCHMKP SEWKPSSSGP
ASGNWTLVDE AGEEDEDPET SWILLNEDDL VTLLSQFPFQ ELFQHLLGFK AKGDYLPETT
RPQEMMKIFA FANSLVELLA VGLDTFNRAR YRQFVKRIGY LIRMTLGYVS DHWAQYVSHS
TGAGLTPQPY SMEKLQVEFD ELFLRAVLHV LKAKRLGIWL FMSEMPFGTL SVQMLWKLLY
LMHQVESGDL QQLCASLQPA ECKRRLQDPE HFASFEKCLS SINSSEEICL LTAFAQMARA
RRTNVDEDFI KIIVLEIYEV SYVTLSTRET FSKVGRELLG AIAAVHPEII SVLLDRVQET
IDQVGMVSLY LFKELPLYLW RPSAPEIAVI RDWLLNNNLT AVKNKLACVI LEGLNWGFTE
QGTLHLDQAL HTEVALLVLE AYQKYLAQKP YTGLISESMK QVSYLASIVR YGETPETSFN
QWAWNLILRL KLHKNDFGRQ NFPVIPFCST VPDMTESSMF HPLLKAVKSG LPIGCYLALA
VTAVGHSLEK FCAEGIPLLG VLVQSRHLRA VVHALDKILP VFYPYQCYLL KNEQFLSNLL
LFLQLDSGVP QGVTQQVTHR VAQHLTGAVH GDNVKLLSSM IQAHICVSTQ PDGVGPVAVL
EFWVQALISQ HLWYREQPIL FLMDHLCKTA FHLMQEDCVQ KLLYQQHKNA LGYHCDRSLL
SSLVNWIVAG NITPSFVEGL STSTQVWFAW TVLNMESIFE EDSQLRRVVE RELVINAFSP
DQALKKAQVQ LKLPIVPSLQ RLLIYRWAHQ ALVTPSDHPL LPLIWQKFFL LYLHRPGPQY
GLPVDGCIGR RFFQSPSHVN LLKDMKRRLT EVADFHYAAS KALRVPAEGS EGTPEGQAGT
PGFLTSPELH RELVRLFNVY VLWLEDENFQ KGDTYIPSLP KHYDVHRLAK VMQNQQDLWM
EYVNMERIQH EFQETVALWT QAKLESHAAP CSSSAQLDFT DPLLAKARVL SNLEKHEAPH
PPLLLHPVRP PVPLIPSAAL LTQKDSTQLM CTDLNLLQQQ ARSATLRESQ QVALDGELLE
TMPKQYVNRE DQATLHLECR GSSGKKCQGA AVVTVQFEGM NKNEAVSQQI HVLQKEVRQL
QAEAAQPPAL NVVEAAVHAE NLITALVNTY KLQPTPGVQK LGISLFFTVV DHVSDETQRH
PPTRQFFTSC IEILGQVFVS GTKSECRKLL QTILKNRRLC SLLAPFFTPN AAPAEFIQLY
ERVVTCLRED NSDVIFMLLT KFDIQQWLNS TKPPLSDRTR LLESIHLALT AWGLEPEEDI
LMPFNLFCKH WTHLLLYQFP DQYSDVLRLL VQSSAEQLLS PECWKATLRA LGCYAPSSQQ
GAASVESSGL HSASRVLLSD KQVMETVQWL SDFFYKLRLS KLDFKSFGLF SKWSPYMADV
KTFLGYLVKR LTDLEIASLS QDPTASSKEV LRSLHAQIIQ LFKPWILVLE DAESSHQRHY
PWLESDTAVA SSIVQLFSDC VGSLHTSFKD RLLPGDEGAL RLHLLHYCET CTAPKMPEFI
LYAFHSAYQR LEWKDLHPDQ RLMEAFFKVE RGSPKSCFLF LGSVLCRVNW VSVLSDAWNP
SPLPETQSMA VCLLFMMVLL AKEAQLVDEP DSPLLSLLGQ TSSLSWHLVD LVSYQSVLGY
FSSHYPPSVV LANDCSSELI VKLLKVSAGL SAHADGRKHV DIVPKCQAFT HQMVQFLSAL
EQTGKITFPA LEREISKLLD DIIIFNPPDM DSQTRHMALS SFFVEVLMMM NNAAVPTAEF
LAVSIRTWIG QRVHGLIVLP LLTAACQSLA SVRHMAEITE ACIMAYFKES SLDQNLGWGP
VLVSLQVPQL TARDFLEECL ALGSCLTLYV YLLQCLNSEQ TVKNDMKMLL VVSGWLEQVY
PSSAQEEAKL FLWWHQILQL SLIQLEQNDS VLTESVIRIL LMLQSRQSLM AEERLSSGIL
GAIGLGRRSP LSNRFRVAAR SMAAFLLVQV PAEDQIRLKP SSELHLAPKA QQVLTALESM
TLSKQYVEYQ DQILHALQFI RHPGHCLQNG KSFLALLVNR LYPEVHYLDN IR
