ID   EPHD_MYCTU              Reviewed;         592 AA.
AC   P9WGS3; L0TBU4; P66777; Q10402;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Probable oxidoreductase EphD;
DE            EC=1.-.-.-;
GN   Name=ephD; OrderedLocusNames=Rv2214c; ORFNames=MTCY190.25c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44991.1; -; Genomic_DNA.
DR   PIR; G70786; G70786.
DR   RefSeq; NP_216730.1; NC_000962.3.
DR   RefSeq; WP_003411445.1; NZ_NVQJ01000008.1.
DR   AlphaFoldDB; P9WGS3; -.
DR   SMR; P9WGS3; -.
DR   STRING; 83332.Rv2214c; -.
DR   PaxDb; P9WGS3; -.
DR   DNASU; 887472; -.
DR   GeneID; 45426190; -.
DR   GeneID; 887472; -.
DR   KEGG; mtu:Rv2214c; -.
DR   TubercuList; Rv2214c; -.
DR   eggNOG; COG1028; Bacteria.
DR   eggNOG; COG2267; Bacteria.
DR   OMA; HTEVPVQ; -.
DR   PhylomeDB; P9WGS3; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProt.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..592
FT                   /note="Probable oxidoreductase EphD"
FT                   /id="PRO_0000054854"
FT   DOMAIN          30..286
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        474
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         461
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   592 AA;  64030 MW;  FF8434097B28D093 CRC64;
     MPATQQMSRL VDSPDGVRIA VYHEGNPDGP TVVLVHGFPD SHVLWDGVVP LLAERFRIVR
     YDNRGVGRSS VPKPISAYTM AHFADDFDAV IGELSPGEPV HVLAHDWGSV GVWEYLRRPG
     ASDRVASFTS VSGPSQDHLV NYVYGGLRRP WRPRTFLRAI SQTLRLSYMA LFSVPVVAPL
     LLRVALSSAA VRRNMVGDIP VDQIHHSETL ARDAAHSVKT YPANYFRSFS SSRRGRAIPI
     VDVPVQLIVN SQDPYVRPYG YDQTARWVPR LWRRDIKAGH FSPMSHPQVM AAAVHDFADL
     ADGKQPSRAL LRAQVGRPRG YFGDTLVSVT GAGSGIGRET ALAFAREGAE IVISDIDEAT
     VKDTAAEIAA RGGIAYPYVL DVSDAEAVEA FAERVSAEHG VPDIVVNNAG IGQAGRFLDT
     PAEQFDRVLA VNLGGVVNGC RAFGQRLVER GTGGHIVNVS SMAAYAPLQS LSAYCTSKAA
     TYMFSDCLRA ELDAAGVGLT TICPGVIDTN IVATTGFHAP GTDEEKIDGR RGQIDKMFAL
     RSYGPDKVAD AIVSAVKKKK PIRPVAPEAY ALYGISRVLP QALRSTARLR VI