EPHX4_HUMAN
ID EPHX4_HUMAN Reviewed; 362 AA.
AC Q8IUS5; Q8NCC6;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Epoxide hydrolase 4;
DE EC=3.3.-.-;
DE AltName: Full=Abhydrolase domain-containing protein 7;
DE AltName: Full=Epoxide hydrolase-related protein;
GN Name=EPHX4; Synonyms=ABHD7, EH4, EPHXRP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PHE-321.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q8IUS5; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-17962164, EBI-8638294;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Epoxide hydrolase
CC family. {ECO:0000305}.
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DR EMBL; AK074822; BAC11230.1; -; mRNA.
DR EMBL; BC041475; AAH41475.1; -; mRNA.
DR CCDS; CCDS736.1; -.
DR RefSeq; NP_775838.3; NM_173567.4.
DR AlphaFoldDB; Q8IUS5; -.
DR SMR; Q8IUS5; -.
DR BioGRID; 128958; 39.
DR IntAct; Q8IUS5; 1.
DR STRING; 9606.ENSP00000359410; -.
DR ESTHER; human-EPHX4; Epoxide_hydrolase.
DR MEROPS; S33.974; -.
DR iPTMnet; Q8IUS5; -.
DR PhosphoSitePlus; Q8IUS5; -.
DR BioMuta; EPHX4; -.
DR DMDM; 134035378; -.
DR EPD; Q8IUS5; -.
DR jPOST; Q8IUS5; -.
DR MassIVE; Q8IUS5; -.
DR MaxQB; Q8IUS5; -.
DR PaxDb; Q8IUS5; -.
DR PeptideAtlas; Q8IUS5; -.
DR PRIDE; Q8IUS5; -.
DR ProteomicsDB; 70611; -.
DR Antibodypedia; 33635; 92 antibodies from 17 providers.
DR DNASU; 253152; -.
DR Ensembl; ENST00000370383.5; ENSP00000359410.4; ENSG00000172031.7.
DR GeneID; 253152; -.
DR KEGG; hsa:253152; -.
DR MANE-Select; ENST00000370383.5; ENSP00000359410.4; NM_173567.5; NP_775838.3.
DR UCSC; uc001don.3; human.
DR CTD; 253152; -.
DR GeneCards; EPHX4; -.
DR HGNC; HGNC:23758; EPHX4.
DR HPA; ENSG00000172031; Tissue enhanced (brain, choroid plexus).
DR MIM; 617401; gene.
DR neXtProt; NX_Q8IUS5; -.
DR OpenTargets; ENSG00000172031; -.
DR PharmGKB; PA164719207; -.
DR VEuPathDB; HostDB:ENSG00000172031; -.
DR eggNOG; KOG4178; Eukaryota.
DR GeneTree; ENSGT00940000156233; -.
DR HOGENOM; CLU_020336_7_3_1; -.
DR InParanoid; Q8IUS5; -.
DR OMA; YRWMVRS; -.
DR OrthoDB; 616687at2759; -.
DR PhylomeDB; Q8IUS5; -.
DR TreeFam; TF314403; -.
DR PathwayCommons; Q8IUS5; -.
DR SignaLink; Q8IUS5; -.
DR BioGRID-ORCS; 253152; 7 hits in 1075 CRISPR screens.
DR ChiTaRS; EPHX4; human.
DR GenomeRNAi; 253152; -.
DR Pharos; Q8IUS5; Tbio.
DR PRO; PR:Q8IUS5; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q8IUS5; protein.
DR Bgee; ENSG00000172031; Expressed in middle temporal gyrus and 107 other tissues.
DR Genevisible; Q8IUS5; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00111; ABHYDROLASE.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..362
FT /note="Epoxide hydrolase 4"
FT /id="PRO_0000281378"
FT TRANSMEM 17..37
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT DOMAIN 94..211
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 169
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 281
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 336
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT VARIANT 321
FT /note="Y -> F (in dbSNP:rs17854127)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_031235"
FT CONFLICT 285
FT /note="F -> I (in Ref. 1; BAC11230)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 362 AA; 42324 MW; B0B359E0AF0EED7C CRC64;
MARLRDCLPR LMLTLRSLLF WSLVYCYCGL CASIHLLKLL WSLGKGPAQT FRRPAREHPP
ACLSDPSLGT HCYVRIKDSG LRFHYVAAGE RGKPLMLLLH GFPEFWYSWR YQLREFKSEY
RVVALDLRGY GETDAPIHRQ NYKLDCLITD IKDILDSLGY SKCVLIGHDW GGMIAWLIAI
CYPEMVMKLI VINFPHPNVF TEYILRHPAQ LLKSSYYYFF QIPWFPEFMF SINDFKVLKH
LFTSHSTGIG RKGCQLTTED LEAYIYVFSQ PGALSGPINH YRNIFSCLPL KHHMVTTPTL
LLWGENDAFM EVEMAEVTKI YVKNYFRLTI LSEASHWLQQ DQPDIVNKLI WTFLKEETRK
KD
