EPI1_PHYIT
ID EPI1_PHYIT Reviewed; 149 AA.
AC D0MVC9;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Extracellular protease inhibitor 1 {ECO:0000303|PubMed:15096512};
DE AltName: Full=Secreted effector EPI1 {ECO:0000303|PubMed:15096512};
DE Flags: Precursor;
GN Name=EPI1 {ECO:0000303|PubMed:15096512}; ORFNames=PITG_22681;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HOST P69B, AND INDUCTION.
RX PubMed=15096512; DOI=10.1074/jbc.m400941200;
RA Tian M., Huitema E., Da Cunha L., Torto-Alalibo T., Kamoun S.;
RT "A Kazal-like extracellular serine protease inhibitor from Phytophthora
RT infestans targets the tomato pathogenesis-related protease P69B.";
RL J. Biol. Chem. 279:26370-26377(2004).
CC -!- FUNCTION: Secreted effector that interacts with and inhibits the
CC pathogenesis-related P69B subtilisin-like serine protease of host
CC tomato (PubMed:15096512). Inhibition of host proteases by a pathogen
CC extracellular protease inhibitor forms a specific type of defense-
CC counterdefense mechanism between plants and microbial pathogens
CC (PubMed:15096512). {ECO:0000269|PubMed:15096512}.
CC -!- SUBUNIT: Interacts with host subtilisin-like protease P69B.
CC {ECO:0000269|PubMed:15096512}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:15096512}.
CC Note=Localizes to host apoplast where it targets defense proteases for
CC inhibition. {ECO:0000269|PubMed:15096512}.
CC -!- INDUCTION: Expressed during host infection with the highest mRNA levels
CC 3 days post-inoculation (PubMed:15096512). EPI1 and its host target
CC P69B are concurrently expressed during infection of tomato by
CC P.infestans (PubMed:15096512). {ECO:0000269|PubMed:15096512}.
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DR EMBL; DS028119; EEY61125.1; -; Genomic_DNA.
DR RefSeq; XP_002908042.1; XM_002907996.1.
DR AlphaFoldDB; D0MVC9; -.
DR MEROPS; I01.040; -.
DR EnsemblProtists; PITG_22681T0; PITG_22681T0; PITG_22681.
DR GeneID; 9468739; -.
DR KEGG; pif:PITG_22681; -.
DR VEuPathDB; FungiDB:PITG_22681; -.
DR eggNOG; ENOG502RGGN; Eukaryota.
DR HOGENOM; CLU_143159_0_0_1; -.
DR InParanoid; D0MVC9; -.
DR OMA; RAKCHNE; -.
DR OrthoDB; 413549at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0048046; C:apoplast; IDA:PHI-base.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:PHI-base.
DR GO; GO:0140403; P:effector-mediated suppression of host innate immune response; IDA:PHI-base.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR Pfam; PF07648; Kazal_2; 2.
DR SMART; SM00280; KAZAL; 2.
DR SUPFAM; SSF100895; SSF100895; 2.
DR PROSITE; PS51465; KAZAL_2; 2.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW Repeat; Secreted; Serine protease inhibitor; Signal; Virulence.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..149
FT /note="Extracellular protease inhibitor 1"
FT /id="PRO_5003012518"
FT DOMAIN 29..86
FT /note="Kazal-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DOMAIN 88..141
FT /note="Kazal-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 41..42
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 100..101
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 35..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 39..58
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 94..124
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 98..117
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 106..139
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ SEQUENCE 149 AA; 16097 MW; 27AD694A4497F188 CRC64;
MKSALLFTLV VAAVHAQSPQ VISPAPRRES NEIDCPEYCL DVYDPVGDGE GNTYSNECYM
KRAKCHNETT PPAWKDLVLI TGSSTGEQPP SKKCSTVCPD VELPVCGSNR VRYGNPCELR
IAACEHPELN IVEDSGKACV GSKVTPQEG
