ID   EPI2A_PHYIT             Reviewed;         125 AA.
AC   D0NBV4; A1L016;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Cystatin-like cysteine protease inhibitor EPIC2A {ECO:0000303|PubMed:17085509};
DE   AltName: Full=Extracellular protease inhibitor with cystatin-like domain protein 2A {ECO:0000303|PubMed:17085509};
DE   AltName: Full=Secreted effector EPIC2A {ECO:0000303|PubMed:17085509};
DE   Flags: Precursor;
GN   Name=EPIC2A {ECO:0000303|PubMed:17085509}; ORFNames=PITG_09175;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=T30-4;
RX   PubMed=17085509; DOI=10.1104/pp.106.090050;
RA   Tian M., Win J., Song J., van der Hoorn R., van der Knaap E., Kamoun S.;
RT   "A Phytophthora infestans cystatin-like protein targets a novel tomato
RT   papain-like apoplastic protease.";
RL   Plant Physiol. 143:364-377(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
CC   -!- FUNCTION: Secreted effector that interacts with and inhibits host
CC       apoplastic pathogenesis-related papain-like cysteine proteases
CC       (Probable). Inhibition of host proteases by a pathogen extracellular
CC       protease inhibitor forms a specific type of defense-counterdefense
CC       mechanism between plants and microbial pathogens (Probable).
CC       {ECO:0000305|PubMed:17085509}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:17085509}.
CC       Note=Localizes to host apoplast where it targets defense proteases for
CC       inhibition. {ECO:0000305|PubMed:17085509}.
CC   -!- INDUCTION: Expression is up-regulated during infection of host tomato.
CC       {ECO:0000269|PubMed:17085509}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AY935251; AAY21182.1; -; mRNA.
DR   EMBL; DS028131; EEY55259.1; -; Genomic_DNA.
DR   RefSeq; XP_002903483.1; XM_002903437.1.
DR   AlphaFoldDB; D0NBV4; -.
DR   SMR; D0NBV4; -.
DR   EnsemblProtists; PITG_09175T0; PITG_09175T0; PITG_09175.
DR   GeneID; 9470510; -.
DR   KEGG; pif:PITG_09175; -.
DR   VEuPathDB; FungiDB:PITG_09175; -.
DR   eggNOG; ENOG502RGHP; Eukaryota.
DR   HOGENOM; CLU_117422_0_0_1; -.
DR   InParanoid; D0NBV4; -.
DR   OrthoDB; 1647595at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR046350; Cystatin_sf.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Protease inhibitor; Reference proteome; Secreted; Signal;
KW   Thiol protease inhibitor; Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..125
FT                   /note="Cystatin-like cysteine protease inhibitor EPIC2A"
FT                   /id="PRO_5003012301"
FT   MOTIF           68..72
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250|UniProtKB:P01040"
FT   SITE            25
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250|UniProtKB:P01040"
FT   CARBOHYD        45
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   125 AA;  13685 MW;  C713DA40841B4FE7 CRC64;
     MSFLRPTLAL LAVTALVTTS AQMNGYTKKE VTPEDMELLQ KAQSNVSAYN RDVTSRICYL
     KVDSLETQVV SGESYKFHVS GCGVNSDKEL GGCANQNCES SKYDIVIYSQ SWTNTLEVTS
     ITPAN