EPIC1_PHYIN
ID EPIC1_PHYIN Reviewed; 126 AA.
AC A1L015;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=Cystatin-like cysteine protease inhibitor EPIC1 {ECO:0000303|PubMed:17085509};
DE AltName: Full=Extracellular protease inhibitor with cystatin-like domain protein 1 {ECO:0000303|PubMed:17085509};
DE AltName: Full=Secreted effector EPIC1 {ECO:0000303|PubMed:17085509};
DE Flags: Precursor;
GN Name=EPIC1 {ECO:0000303|PubMed:17085509};
OS Phytophthora infestans (Potato late blight agent) (Botrytis infestans).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=4787;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Isolate 88069;
RX PubMed=17085509; DOI=10.1104/pp.106.090050;
RA Tian M., Win J., Song J., van der Hoorn R., van der Knaap E., Kamoun S.;
RT "A Phytophthora infestans cystatin-like protein targets a novel tomato
RT papain-like apoplastic protease.";
RL Plant Physiol. 143:364-377(2007).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HOST RCR3.
RX PubMed=19171904; DOI=10.1073/pnas.0809201106;
RA Song J., Win J., Tian M., Schornack S., Kaschani F., Ilyas M.,
RA van der Hoorn R.A., Kamoun S.;
RT "Apoplastic effectors secreted by two unrelated eukaryotic plant pathogens
RT target the tomato defense protease Rcr3.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:1654-1659(2009).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HOST C14.
RX PubMed=20940351; DOI=10.1104/pp.110.158030;
RA Kaschani F., Shabab M., Bozkurt T., Shindo T., Schornack S., Gu C.,
RA Ilyas M., Win J., Kamoun S., van der Hoorn R.A.;
RT "An effector-targeted protease contributes to defense against Phytophthora
RT infestans and is under diversifying selection in natural hosts.";
RL Plant Physiol. 154:1794-1804(2010).
RN [4]
RP EPIC1-C14 COMPLEX STRUCTURE MODELING.
RX PubMed=21301220; DOI=10.4161/psb.6.1.14190;
RA Kaschani F., Van der Hoorn R.A.;
RT "A model of the C14-EPIC complex indicates hotspots for a protease-
RT inhibitor arms race in the oomycete-potato interaction.";
RL Plant Signal. Behav. 6:109-112(2011).
CC -!- FUNCTION: Secreted effector that interacts with and inhibits the
CC pathogenesis-related papain-like cysteine proteases C14 and RCR3 of
CC host plants (PubMed:17085509, PubMed:19171904). Inhibition of host
CC proteases by a pathogen extracellular protease inhibitor forms a
CC specific type of defense-counterdefense mechanism between plants and
CC microbial pathogens (PubMed:17085509). {ECO:0000269|PubMed:17085509,
CC ECO:0000269|PubMed:19171904}.
CC -!- SUBUNIT: Interacts with the host papain-like cysteine protease RCR3
CC (PubMed:19171904). Interacts with the host papain-like cysteine
CC protease C14 (PubMed:20940351) (Probable).
CC {ECO:0000269|PubMed:19171904, ECO:0000269|PubMed:20940351,
CC ECO:0000305|PubMed:21301220}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17085509,
CC ECO:0000269|PubMed:19171904, ECO:0000269|PubMed:20940351}.
CC Note=Localizes to host apoplast where it targets defense proteases for
CC inhibition. {ECO:0000269|PubMed:17085509, ECO:0000269|PubMed:19171904,
CC ECO:0000269|PubMed:20940351}.
CC -!- INDUCTION: Expression is up-regulated during infection of host tomato.
CC {ECO:0000269|PubMed:17085509}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; AY935250; AAY21181.1; -; mRNA.
DR AlphaFoldDB; A1L015; -.
DR SMR; A1L015; -.
DR DIP; DIP-48694N; -.
DR IntAct; A1L015; 1.
DR MEROPS; I25.047; -.
DR VEuPathDB; FungiDB:PITG_09169; -.
DR OMA; AITDAPW; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR046350; Cystatin_sf.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Protease inhibitor; Secreted; Signal;
KW Thiol protease inhibitor; Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..126
FT /note="Cystatin-like cysteine protease inhibitor EPIC1"
FT /id="PRO_0000448005"
FT MOTIF 69..73
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250|UniProtKB:P01040"
FT SITE 25
FT /note="Reactive site"
FT /evidence="ECO:0000250|UniProtKB:P01040"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 126 AA; 13711 MW; D4021C6B18020532 CRC64;
MTFLRPILAL LAATALVTTS AQVDGGYSKK EVTPEDMELL QKAQSNVSAY NSDVTSRICY
LKVDSLETQV VSGENYKFHV SGCSVNSDNE LGGCANQNCE SSKYDIVIYS QSWTNTLEVT
SITPVK
