ID   EPIC3_PHYIN             Reviewed;         131 AA.
AC   A1L018;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Cystatin-like cysteine protease inhibitor EPIC3 {ECO:0000303|PubMed:17085509};
DE   AltName: Full=Extracellular protease inhibitor with cystatin-like domain protein 3 {ECO:0000303|PubMed:17085509};
DE   AltName: Full=Secreted effector EPIC3 {ECO:0000303|PubMed:17085509};
DE   Flags: Precursor;
GN   Name=EPIC3 {ECO:0000303|PubMed:17085509};
OS   Phytophthora infestans (Potato late blight agent) (Botrytis infestans).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4787;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   STRAIN=90128;
RX   PubMed=17085509; DOI=10.1104/pp.106.090050;
RA   Tian M., Win J., Song J., van der Hoorn R., van der Knaap E., Kamoun S.;
RT   "A Phytophthora infestans cystatin-like protein targets a novel tomato
RT   papain-like apoplastic protease.";
RL   Plant Physiol. 143:364-377(2007).
CC   -!- FUNCTION: Secreted effector that interacts with and inhibits host
CC       apoplastic pathogenesis-related papain-like cysteine proteases
CC       (Probable). Inhibition of host proteases by a pathogen extracellular
CC       protease inhibitor forms a specific type of defense-counterdefense
CC       mechanism between plants and microbial pathogens (Probable).
CC       {ECO:0000305|PubMed:17085509}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:17085509}.
CC       Note=Localizes to host apoplast where it targets defense proteases for
CC       inhibition. {ECO:0000305|PubMed:17085509}.
CC   -!- INDUCTION: Expressed during infection of host plant and in germinating
CC       cysts. {ECO:0000269|PubMed:17085509}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AY935253; AAY21184.1; -; mRNA.
DR   AlphaFoldDB; A1L018; -.
DR   SMR; A1L018; -.
DR   VEuPathDB; FungiDB:PITG_14891; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Protease inhibitor; Secreted; Signal;
KW   Thiol protease inhibitor; Virulence.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..131
FT                   /note="Cystatin-like cysteine protease inhibitor EPIC3"
FT                   /id="PRO_5002635797"
FT   MOTIF           71..75
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250|UniProtKB:P01040"
FT   SITE            27
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250|UniProtKB:P01040"
FT   CARBOHYD        33
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   131 AA;  14125 MW;  683025F69DB5C3E1 CRC64;
     MAFTRSIALF AGLALAASSA QEATILGGYT QKNATSDDIE LLTQATSSAN MYNKNVDTRI
     CLIAIENLET QTVAGTNYKF QVAGCPVETD DELGACDDRN CDYSSYNIVI FSQPWSDTIE
     VTSITPAEYQ G