EPIC3_PHYIT
ID EPIC3_PHYIT Reviewed; 131 AA.
AC D0NP95;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=Cystatin-like cysteine protease inhibitor EPIC3 {ECO:0000303|PubMed:17085509};
DE AltName: Full=Extracellular protease inhibitor with cystatin-like domain protein 3 {ECO:0000303|PubMed:17085509};
DE AltName: Full=Secreted effector EPIC3 {ECO:0000303|PubMed:17085509};
DE Flags: Precursor;
GN Name=EPIC3 {ECO:0000303|PubMed:17085509}; ORFNames=PITG_14891;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP IDENTIFICATION, AND INDUCTION.
RX PubMed=17085509; DOI=10.1104/pp.106.090050;
RA Tian M., Win J., Song J., van der Hoorn R., van der Knaap E., Kamoun S.;
RT "A Phytophthora infestans cystatin-like protein targets a novel tomato
RT papain-like apoplastic protease.";
RL Plant Physiol. 143:364-377(2007).
CC -!- FUNCTION: Secreted effector that interacts with and inhibits host
CC apoplastic pathogenesis-related papain-like cysteine proteases
CC (Probable). Inhibition of host proteases by a pathogen extracellular
CC protease inhibitor forms a specific type of defense-counterdefense
CC mechanism between plants and microbial pathogens (Probable).
CC {ECO:0000305|PubMed:17085509}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:17085509}.
CC Note=Localizes to host apoplast where it targets defense proteases for
CC inhibition. {ECO:0000305|PubMed:17085509}.
CC -!- INDUCTION: Expressed during infection of host plant and in germinating
CC cysts. {ECO:0000269|PubMed:17085509}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; DS028150; EEY62437.1; -; Genomic_DNA.
DR RefSeq; XP_002899073.1; XM_002899027.1.
DR AlphaFoldDB; D0NP95; -.
DR SMR; D0NP95; -.
DR EnsemblProtists; PITG_14891T0; PITG_14891T0; PITG_14891.
DR GeneID; 9467999; -.
DR KEGG; pif:PITG_14891; -.
DR VEuPathDB; FungiDB:PITG_14891; -.
DR eggNOG; ENOG502SST5; Eukaryota.
DR HOGENOM; CLU_117422_0_0_1; -.
DR InParanoid; D0NP95; -.
DR OMA; FQVAGCP; -.
DR OrthoDB; 1565344at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Protease inhibitor; Reference proteome; Secreted; Signal;
KW Thiol protease inhibitor; Virulence.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..131
FT /note="Cystatin-like cysteine protease inhibitor EPIC3"
FT /id="PRO_5003013612"
FT MOTIF 71..75
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250|UniProtKB:P01040"
FT SITE 27
FT /note="Reactive site"
FT /evidence="ECO:0000250|UniProtKB:P01040"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 131 AA; 14053 MW; 8586FE98A55763C9 CRC64;
MAFTRSIALF AGLALAASSA QGATILGGYT QKNATSDDIE LLTQATSSAN MYNKNVDTRI
CLIAIENLET QTVAGTNYKF QVAGCPVETD DELGACDDRN CDYSSYNIVI FSQPWSDTIE
VTSITPAEYQ G
