ID   EPIC4_PHYIT             Reviewed;         172 AA.
AC   D0MSS2;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Cystatin-like cysteine protease inhibitor EPIC4 {ECO:0000303|PubMed:17085509};
DE   AltName: Full=Extracellular protease inhibitor with cystatin-like domain protein 4 {ECO:0000303|PubMed:17085509};
DE   AltName: Full=Secreted effector EPIC4 {ECO:0000303|PubMed:17085509};
DE   Flags: Precursor;
GN   Name=EPIC4 {ECO:0000303|PubMed:17085509}; ORFNames=PITG_00058;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   IDENTIFICATION, AND INDUCTION.
RX   PubMed=17085509; DOI=10.1104/pp.106.090050;
RA   Tian M., Win J., Song J., van der Hoorn R., van der Knaap E., Kamoun S.;
RT   "A Phytophthora infestans cystatin-like protein targets a novel tomato
RT   papain-like apoplastic protease.";
RL   Plant Physiol. 143:364-377(2007).
CC   -!- FUNCTION: Secreted effector that interacts with and inhibits host
CC       apoplastic pathogenesis-related papain-like cysteine proteases
CC       (Probable). Inhibition of host proteases by a pathogen extracellular
CC       protease inhibitor forms a specific type of defense-counterdefense
CC       mechanism between plants and microbial pathogens (Probable).
CC       {ECO:0000305|PubMed:17085509}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:17085509}.
CC       Note=Localizes to host apoplast where it targets defense proteases for
CC       inhibition. {ECO:0000305|PubMed:17085509}.
CC   -!- INDUCTION: Expressed during infection of host plant and non-sporulating
CC       growth, upon nitrogen starvation, in mating culture, in mycelium as
CC       well as in ungerminated sporangia. {ECO:0000269|PubMed:17085509}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; DS028118; EEY57506.1; -; Genomic_DNA.
DR   RefSeq; XP_002908692.1; XM_002908646.1.
DR   AlphaFoldDB; D0MSS2; -.
DR   SMR; D0MSS2; -.
DR   EnsemblProtists; PITG_00058T0; PITG_00058T0; PITG_00058.
DR   GeneID; 9476578; -.
DR   KEGG; pif:PITG_00058; -.
DR   VEuPathDB; FungiDB:PITG_00058; -.
DR   eggNOG; ENOG502S15D; Eukaryota.
DR   HOGENOM; CLU_117422_0_0_1; -.
DR   InParanoid; D0MSS2; -.
DR   OMA; CAPTEYV; -.
DR   OrthoDB; 1565344at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Protease inhibitor; Reference proteome; Secreted; Signal;
KW   Thiol protease inhibitor; Virulence.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..172
FT                   /note="Cystatin-like cysteine protease inhibitor EPIC4"
FT                   /id="PRO_5003012437"
FT   REGION          129..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           71..75
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250|UniProtKB:P01040"
FT   SITE            27
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250|UniProtKB:P01040"
SQ   SEQUENCE   172 AA;  18014 MW;  97164AEB17BA606A CRC64;
     MRASLSILVA FPALAAARDV VTIGMTGSWH PADVTEDNTK LLGTALSGSS FSKSVGDKRV
     CYSEVTSLET QVVAGTNYRF HISGCDVTDS DGECSTSALS SCELSGFVVQ IFEQSWTNTL
     KVTNIKAEEA ATASSSSTPA PTPASTSTSA SSSEETMLQS SVQQRAMFSD FV