ID   AGO2_ORYSJ              Reviewed;        1034 AA.
AC   Q7XTS4; A0A0P0WEU6;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Protein argonaute 2;
DE            Short=OsAGO2;
GN   Name=AGO2; OrderedLocusNames=Os04g0615700, LOC_Os04g52540;
GN   ORFNames=OSJNBa0008M17.11;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18826656; DOI=10.1186/1471-2164-9-451;
RA   Kapoor M., Arora R., Lama T., Nijhawan A., Khurana J.P., Tyagi A.K.,
RA   Kapoor S.;
RT   "Genome-wide identification, organization and phylogenetic analysis of
RT   dicer-like, argonaute and RNA-dependent RNA polymerase gene families and
RT   their expression analysis during reproductive development and stress in
RT   rice.";
RL   BMC Genomics 9:451-451(2008).
CC   -!- FUNCTION: Probably involved in the RNA silencing pathway. May bind to
CC       short RNAs such as microRNAs (miRNAs) or short interfering RNAs
CC       (siRNAs), and represses the translation of mRNAs which are
CC       complementary to them (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL662950; CAD41795.2; -; Genomic_DNA.
DR   EMBL; AP008210; BAF15785.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS91002.1; -; Genomic_DNA.
DR   EMBL; AK120766; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015636011.1; XM_015780525.1.
DR   AlphaFoldDB; Q7XTS4; -.
DR   SMR; Q7XTS4; -.
DR   STRING; 4530.OS04T0615700-01; -.
DR   iPTMnet; Q7XTS4; -.
DR   PaxDb; Q7XTS4; -.
DR   PRIDE; Q7XTS4; -.
DR   EnsemblPlants; Os04t0615700-01; Os04t0615700-01; Os04g0615700.
DR   GeneID; 4336991; -.
DR   Gramene; Os04t0615700-01; Os04t0615700-01; Os04g0615700.
DR   KEGG; osa:4336991; -.
DR   eggNOG; KOG1041; Eukaryota.
DR   HOGENOM; CLU_004544_3_0_1; -.
DR   InParanoid; Q7XTS4; -.
DR   OMA; YLCSYYG; -.
DR   OrthoDB; 159407at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q7XTS4; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR   CDD; cd04657; Piwi_ago-like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR014811; ArgoL1.
DR   InterPro; IPR032472; ArgoL2.
DR   InterPro; IPR032474; Argonaute_N.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR045246; Piwi_ago-like.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF08699; ArgoL1; 1.
DR   Pfam; PF16488; ArgoL2; 1.
DR   Pfam; PF16486; ArgoN; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM01163; DUF1785; 1.
DR   SMART; SM00949; PAZ; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..1034
FT                   /note="Protein argonaute 2"
FT                   /id="PRO_0000378429"
FT   DOMAIN          390..504
FT                   /note="PAZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT   DOMAIN          688..989
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT   REGION          1..201
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        948
FT                   /note="D -> N (in Ref. 5; AK120766)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1034 AA;  111445 MW;  596847313CAD09CE CRC64;
     MEHERGGGGR GRGRGRGGGR GGGGGDGRGG GYGGAGGGGV GGRGGRGPPG GGGGRGYEPG
     GGRGYGGGGG GGGRGYGGGG GGGGYESGGG RGYGGGGRGY ESGGGRGPGG GGRGHESGGG
     GGRGGNVWAQ PGRGRGGAPA PAPAPAPAAR RIQDEGAARS SGTVERIAST EVVRVQPPAP
     PVAVSRSGTR VPMRRPDGGG SVSKAKVKLL VNHFIVKYRQ ASTVFHYDID IKLDISSPKA
     SDKELSKGDF LTVKDELFKD ESFRRLSSAV AYDGKRNLFT CAELPDGLFR VKVRSRTYIV
     SVEFKKKLPL SQLSELPVPR EVLQGLDVIV REASSWRKII IGQGFYSQGR SVPIGPDVVA
     LKGTQQTLKC TQKGLILCVD YSVMPFRKAG PVLDLVQKSV RYLDYRTTLN KHQLDTLKNE
     LKGQRVTVNH RRTKQKYIVK GLTDKPASQI TFVDSESGQT KKLLDYYSQQ YGKVIEYQML
     PCLDLSKSKD KQNYVPIELC DLLEGQRYPK ASLNRNSDKT LKEMALIPAS SRKEEILELV
     NADDGPCRGE IAQQFGISLD VQMMEVTGRT LPPPSLKLGT SSGQPPKFNI DQPNCQWNLT
     RKRLAEGGVL QCWGVVDFSA DSGQYALNGN MFIDKIVRKC CDLGVQMNRN PCIVQLLDME
     VLSDPHQLFE ELNKAKQAAA SKKQKLQLLF CPMSDQHPGY KTLKLICETQ LGIQTQCFLS
     FLANKQQGQD QYMSNLALKI NGKIGGSNIQ LFGESLPRIS GAPYMFIGAD VNHPSPGNVE
     SPSIAAVVAS VDQGASKYVP RIRAQPHRCE VIQHLGDMCK ELIGVFEKRN RVKPQRIIYF
     RDGVSDGQFD MVLNEELADM EKAIKTKDYS PTITVIVAKK RHHTRLFPKD LNQQQTKNGN
     VLPGTVVDTG VVDPAAYDFY LCSHNGLIGT SRPTHYYSLL DEHGFASDDL QKLVYNLCFV
     FARCTKPVSL ATPVYYADLA AYRGRLYYEG MMMSQPPPSS AASASSASSS GAGASDFRSF
     PALHEDLVDN MFFI