AGO3_ARATH
ID AGO3_ARATH Reviewed; 1194 AA.
AC Q9SHF2;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Protein argonaute 3;
GN Name=AGO3; OrderedLocusNames=At1g31290; ORFNames=T19E23.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Involved in RNA-mediated post-transcriptional gene silencing
CC (PTGS). Main component of the RNA-induced silencing complex (RISC) that
CC binds to a short guide RNA such as a microRNA (miRNA) or small
CC interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide
CC for slicer-directed cleavage of homologous mRNAs to repress gene
CC expression (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC {ECO:0000305}.
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DR EMBL; AC007654; AAF24586.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31336.1; -; Genomic_DNA.
DR RefSeq; NP_174414.1; NM_102867.2.
DR AlphaFoldDB; Q9SHF2; -.
DR SMR; Q9SHF2; -.
DR STRING; 3702.AT1G31290.1; -.
DR PaxDb; Q9SHF2; -.
DR PRIDE; Q9SHF2; -.
DR ProteomicsDB; 244773; -.
DR EnsemblPlants; AT1G31290.1; AT1G31290.1; AT1G31290.
DR GeneID; 840017; -.
DR Gramene; AT1G31290.1; AT1G31290.1; AT1G31290.
DR KEGG; ath:AT1G31290; -.
DR Araport; AT1G31290; -.
DR TAIR; locus:2197550; AT1G31290.
DR eggNOG; KOG1041; Eukaryota.
DR HOGENOM; CLU_004544_3_0_1; -.
DR InParanoid; Q9SHF2; -.
DR OrthoDB; 220258at2759; -.
DR PhylomeDB; Q9SHF2; -.
DR PRO; PR:Q9SHF2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SHF2; baseline and differential.
DR Genevisible; Q9SHF2; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0051607; P:defense response to virus; IDA:TAIR.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd04657; Piwi_ago-like; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR014811; ArgoL1.
DR InterPro; IPR032472; ArgoL2.
DR InterPro; IPR032474; Argonaute_N.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR003165; Piwi.
DR InterPro; IPR045246; Piwi_ago-like.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF08699; ArgoL1; 1.
DR Pfam; PF16488; ArgoL2; 1.
DR Pfam; PF16486; ArgoN; 1.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF02171; Piwi; 1.
DR SMART; SM01163; DUF1785; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00950; Piwi; 1.
DR SUPFAM; SSF101690; SSF101690; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS50822; PIWI; 1.
PE 3: Inferred from homology;
KW Plant defense; Reference proteome; Repressor; Ribonucleoprotein;
KW RNA-binding; RNA-mediated gene silencing; Transcription;
KW Transcription regulation; Translation regulation.
FT CHAIN 1..1194
FT /note="Protein argonaute 3"
FT /id="PRO_0000404666"
FT DOMAIN 540..656
FT /note="PAZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT DOMAIN 841..1145
FT /note="Piwi"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT REGION 1..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..55
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..277
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..326
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1194 AA; 129185 MW; 930E52C6CDBE2AB2 CRC64;
MDRGGYRGGR GDGRGRGGGG DRGRGYSGRG DGRGRGGDGD RGYSGRGDGH GRGGGGDRGR
GYSGRGDGRG RGGGGDRGRG YSGRGDGHGR GGGGDRGRGY SGRGRGFVQD RDGGWVNPGQ
SSGGHVRGRG TQLQQPPPQE VPPSSSQAQV SQGVAPGDVG QGGVGDVGRD GVGDVGRDGV
GDVGQGGVGD VGQVGVGDVG QGGVGDVGQG GVGDVGRDGV GDVGRDGVGD VGRGGVGDRG
QSQSGLSSGH FGRGTQLQQP QPQAVSQSSS QGQVSQSFAT GGVGLGAWAR KPQLFSDSTV
LPSSSSSNVV ASHTASGSQV MTPKPSSSDK KEPVKRPDKG GNIKVKGVIN LSVNHFRVSF
STESVIRHYD VDIKGENSSK KISRFELAMV KEKLFKDNND FPNAMTAYDG QKNIFSAVEL
PTGSFKVDFS ETEEIMRGRS YTFIIKQVKE LKLLDLQAYI DGRSTFIPRD VLQGMDVVMK
EHPSKRMITV GKRFFSTRLE IDFGYGVGAA KGFHHTLKPT VQGLSLCLNS SLLAFRKAIS
VIEYLKLYFG WRNIRQFKNC RPDDVVQELI GLKVTVDHRK TKQKFIIMGL SKDDTKDIKF
DFIDHAGNQP PRKISIVEYF KEKYGRDIDH KDIPCLNLGK KGRENFVPME FCNLVEGQIF
PKEKLYRDSA AWLKELSLVT PQQRLENINK MIKSSDGPRG GDIIGNFGLR VDPNMTTVEG
RVLEAPTLKL TDRRGNPIHE KLMSESNQWN LTTKGVTKGS IIKHWAVLDF TASESLKKKM
PGYFVNKLIE RCKGLGMQME APIVCKTSSM ETLYDGNALE ELLRSVIDEA SHNHGGACPT
LVLCAMTGKH DGYKTLKWIA ETKLGLVTQC FLTISAIKGE TVSDQYLANL ALKINAKVGG
TNVELVDNIF SFFKKEDKVM FIGADVNHPA AHDNMSPSIV AVVGTLNWPE ANRYAARVKA
QSHRKEEIQG FGETCWELIE AHSQAPEKRP NKIVIFRDGV SDGQFDMVLN VELQNVKDVF
AKVGYNPQIT VIVAQKRHQT RFFPATTSKD GRAKGNVPSG TVVDTTIIHP FEYDFYLCSQ
HGAIGTSKPT HYYVLSDEIG FNSNQIQKLI FDLCFTFTRC TKPVALVPPV SYADKAASRG
RVYYEASLMK KNSKQSRGAS SSSASVASSS SSVTMEDKEI FKVHAGIENF MFFV
