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EPN2_MOUSE
ID   EPN2_MOUSE              Reviewed;         595 AA.
AC   Q8CHU3; O70447; Q5NCM4; Q8BZ85;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 165.
DE   RecName: Full=Epsin-2;
DE   AltName: Full=EPS-15-interacting protein 2;
DE   AltName: Full=Intersectin-EH-binding protein 2;
DE            Short=Ibp2;
GN   Name=Epn2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 75-595 (ISOFORM 2), AND INTERACTION WITH
RP   ITSN1.
RX   PubMed=9813051; DOI=10.1074/jbc.273.47.31401;
RA   Yamabhai M., Hoffman N.G., Hardison N.L., McPherson P.S., Castagnoli L.,
RA   Cesareni G., Kay B.K.;
RT   "Intersectin, a novel adaptor protein with two eps15 homology and five src
RT   homology 3 domains.";
RL   J. Biol. Chem. 273:31401-31407(1998).
RN   [5]
RP   PROTEIN SEQUENCE OF 77-86, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=OF1; TISSUE=Hippocampus;
RA   Lubec G., Sunyer B., Chen W.-Q.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Embryonic brain;
RX   PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA   Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT   "Phosphoproteomic analysis of the developing mouse brain.";
RL   Mol. Cell. Proteomics 3:1093-1101(2004).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173 AND SER-192, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC   Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [8]
RP   METHYLATION [LARGE SCALE ANALYSIS] AT ARG-170, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain;
RX   PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA   Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA   Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA   Bedford M.T., Comb M.J.;
RT   "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT   methylation.";
RL   Mol. Cell. Proteomics 13:372-387(2014).
CC   -!- FUNCTION: Plays a role in the formation of clathrin-coated
CC       invaginations and endocytosis. {ECO:0000250}.
CC   -!- SUBUNIT: Binds EPS15, AP-2 and clathrin (By similarity). Interacts with
CC       UBQLN2 (By similarity). Interacts with ITSN1.
CC       {ECO:0000250|UniProtKB:O95208, ECO:0000250|UniProtKB:Q9Z1Z3,
CC       ECO:0000269|PubMed:9813051}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=In punctate
CC       structures throughout the cell and particularly concentrated in the
CC       region of the Golgi complex. {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8CHU3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8CHU3-2; Sequence=VSP_009157;
CC   -!- DOMAIN: The NPF repeat domain is involved in EPS15 binding.
CC   -!- DOMAIN: The DPW repeat domain is involved in AP-2 and clathrin binding.
CC   -!- PTM: Ubiquitinated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the epsin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC97476.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AK036331; BAC29387.1; -; mRNA.
DR   EMBL; AL604029; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC039138; AAH39138.1; -; mRNA.
DR   EMBL; AF057286; AAC97476.1; ALT_INIT; mRNA.
DR   CCDS; CCDS24816.1; -. [Q8CHU3-1]
DR   CCDS; CCDS56779.1; -. [Q8CHU3-2]
DR   RefSeq; NP_001239118.1; NM_001252189.1. [Q8CHU3-2]
DR   RefSeq; NP_034278.1; NM_010148.3. [Q8CHU3-1]
DR   RefSeq; XP_017169742.1; XM_017314253.1.
DR   AlphaFoldDB; Q8CHU3; -.
DR   SMR; Q8CHU3; -.
DR   BioGRID; 199486; 8.
DR   ELM; Q8CHU3; -.
DR   IntAct; Q8CHU3; 1.
DR   MINT; Q8CHU3; -.
DR   STRING; 10090.ENSMUSP00000001063; -.
DR   iPTMnet; Q8CHU3; -.
DR   PhosphoSitePlus; Q8CHU3; -.
DR   jPOST; Q8CHU3; -.
DR   MaxQB; Q8CHU3; -.
DR   PeptideAtlas; Q8CHU3; -.
DR   PRIDE; Q8CHU3; -.
DR   ProteomicsDB; 275634; -. [Q8CHU3-1]
DR   ProteomicsDB; 275635; -. [Q8CHU3-2]
DR   Antibodypedia; 25881; 348 antibodies from 27 providers.
DR   DNASU; 13855; -.
DR   Ensembl; ENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036. [Q8CHU3-1]
DR   Ensembl; ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036. [Q8CHU3-2]
DR   Ensembl; ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036. [Q8CHU3-1]
DR   GeneID; 13855; -.
DR   KEGG; mmu:13855; -.
DR   UCSC; uc007jhv.2; mouse. [Q8CHU3-1]
DR   CTD; 22905; -.
DR   MGI; MGI:1333766; Epn2.
DR   VEuPathDB; HostDB:ENSMUSG00000001036; -.
DR   eggNOG; KOG2056; Eukaryota.
DR   GeneTree; ENSGT00940000157239; -.
DR   HOGENOM; CLU_012678_4_2_1; -.
DR   InParanoid; Q8CHU3; -.
DR   OrthoDB; 1263849at2759; -.
DR   PhylomeDB; Q8CHU3; -.
DR   TreeFam; TF313361; -.
DR   Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR   Reactome; R-MMU-8856828; Clathrin-mediated endocytosis.
DR   BioGRID-ORCS; 13855; 3 hits in 71 CRISPR screens.
DR   ChiTaRS; Epn2; mouse.
DR   PRO; PR:Q8CHU3; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; Q8CHU3; protein.
DR   Bgee; ENSMUSG00000001036; Expressed in animal zygote and 254 other tissues.
DR   ExpressionAtlas; Q8CHU3; baseline and differential.
DR   Genevisible; Q8CHU3; MM.
DR   GO; GO:0030128; C:clathrin coat of endocytic vesicle; ISO:MGI.
DR   GO; GO:0030125; C:clathrin vesicle coat; IBA:GO_Central.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0030276; F:clathrin binding; IBA:GO_Central.
DR   GO; GO:0005543; F:phospholipid binding; IBA:GO_Central.
DR   GO; GO:0048568; P:embryonic organ development; IGI:MGI.
DR   GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR   GO; GO:0001701; P:in utero embryonic development; IGI:MGI.
DR   GO; GO:1903671; P:negative regulation of sprouting angiogenesis; ISO:MGI.
DR   GO; GO:0030948; P:negative regulation of vascular endothelial growth factor receptor signaling pathway; ISO:MGI.
DR   GO; GO:0007219; P:Notch signaling pathway; IGI:MGI.
DR   GO; GO:0045747; P:positive regulation of Notch signaling pathway; ISO:MGI.
DR   GO; GO:0030100; P:regulation of endocytosis; ISO:MGI.
DR   Gene3D; 1.25.40.90; -; 1.
DR   InterPro; IPR013809; ENTH.
DR   InterPro; IPR008942; ENTH_VHS.
DR   InterPro; IPR027319; Epsin-2_metazoa.
DR   InterPro; IPR003903; UIM_dom.
DR   PANTHER; PTHR12276:SF50; PTHR12276:SF50; 1.
DR   Pfam; PF01417; ENTH; 1.
DR   SMART; SM00273; ENTH; 1.
DR   SMART; SM00726; UIM; 2.
DR   SUPFAM; SSF48464; SSF48464; 1.
DR   PROSITE; PS50942; ENTH; 1.
DR   PROSITE; PS50330; UIM; 2.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Direct protein sequencing; Endocytosis;
KW   Lipid-binding; Methylation; Phosphoprotein; Reference proteome; Repeat;
KW   Ubl conjugation.
FT   CHAIN           1..595
FT                   /note="Epsin-2"
FT                   /id="PRO_0000074517"
FT   DOMAIN          12..144
FT                   /note="ENTH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT   DOMAIN          218..237
FT                   /note="UIM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT   DOMAIN          255..274
FT                   /note="UIM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT   REPEAT          313..315
FT                   /note="1"
FT   REPEAT          325..327
FT                   /note="2"
FT   REPEAT          338..340
FT                   /note="3"
FT   REPEAT          352..354
FT                   /note="4"
FT   REPEAT          370..372
FT                   /note="5"
FT   REPEAT          387..389
FT                   /note="6"
FT   REPEAT          494..496
FT                   /note="1"
FT   REPEAT          508..510
FT                   /note="2"
FT   REPEAT          591..593
FT                   /note="3"
FT   REGION          164..254
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          267..289
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          305..396
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          313..389
FT                   /note="6 X 3 AA repeats of [DE]-P-W"
FT   REGION          423..469
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          494..593
FT                   /note="3 X 3 AA repeats of N-P-F"
FT   COMPBIAS        164..186
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..221
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        267..284
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        305..341
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        355..384
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        434..467
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         8
FT                   /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT                   inositol-4,5-bisphosphate)"
FT                   /ligand_id="ChEBI:CHEBI:58456"
FT                   /evidence="ECO:0000250"
FT   BINDING         11
FT                   /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT                   inositol-4,5-bisphosphate)"
FT                   /ligand_id="ChEBI:CHEBI:58456"
FT                   /evidence="ECO:0000250"
FT   BINDING         25
FT                   /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT                   inositol-4,5-bisphosphate)"
FT                   /ligand_id="ChEBI:CHEBI:58456"
FT                   /evidence="ECO:0000250"
FT   BINDING         30
FT                   /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT                   inositol-4,5-bisphosphate)"
FT                   /ligand_id="ChEBI:CHEBI:58456"
FT                   /evidence="ECO:0000250"
FT   BINDING         63
FT                   /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT                   inositol-4,5-bisphosphate)"
FT                   /ligand_id="ChEBI:CHEBI:58456"
FT                   /evidence="ECO:0000250"
FT   BINDING         73
FT                   /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT                   inositol-4,5-bisphosphate)"
FT                   /ligand_id="ChEBI:CHEBI:58456"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         170
FT                   /note="Omega-N-methylarginine"
FT                   /evidence="ECO:0007744|PubMed:24129315"
FT   MOD_RES         173
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         192
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         195
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Z1Z3"
FT   MOD_RES         443
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Z1Z3"
FT   MOD_RES         465
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Z1Z3"
FT   MOD_RES         526
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O95208"
FT   VAR_SEQ         237..248
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072,
FT                   ECO:0000303|PubMed:9813051"
FT                   /id="VSP_009157"
FT   CONFLICT        75
FT                   /note="Y -> S (in Ref. 4; AAC97476)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   595 AA;  63472 MW;  255DC7BC7E1B2D13 CRC64;
     MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV VAFSEIMSMV
     WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI FAIQTLKDFQ YIDRDGKDQG
     INVREKSKQL VALLKDEERL KVERVQALKT KERMAQVATG VGSNQITFGR GSSQPNLSTS
     YSEQEYGKAG GSPASYHGST SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES
     VQTARGSKEE RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD
     ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS VDPWGVPTTA
     STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS FELFSNFNGT VKDDFSEFDN
     LRTSKKPAES GASVPPQDSR TTSPDLFESQ SLTSASSKPS SARKTPESFL GPNAALVNLD
     SLVTKPAPPA QSLNPFLAPG AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG
     VETVAPMTSV APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL
 
 
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