ID   EPO_BOSMU               Reviewed;         192 AA.
AC   A3FFS8; A3FM64;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Erythropoietin;
DE   Flags: Precursor;
GN   Name=EPO;
OS   Bos mutus grunniens (Wild yak) (Bos grunniens).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=30521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LEU-86.
RC   STRAIN=BaZhou, DaTong, and JiuLong;
RA   Jin S., Zhong J.C., Chen Z.H., Zhuo Y.;
RT   "Cloning and sequence analysis on erythropoietin (EPO) gene of Bos
RT   grunniens.";
RL   Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC       proliferation and differentiation and the maintenance of a
CC       physiological level of circulating erythrocyte mass. Binds to EPOR
CC       leading to EPOR dimerization and JAK2 activation thereby activating
CC       specific downstream effectors, including STAT1 and STAT3.
CC       {ECO:0000250|UniProtKB:P01588}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC       liver of fetal or neonatal mammals.
CC   -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR   EMBL; EF374047; ABN48307.1; -; Genomic_DNA.
DR   EMBL; EF374048; ABN48308.1; -; Genomic_DNA.
DR   EMBL; EF405921; ABN59378.1; -; Genomic_DNA.
DR   AlphaFoldDB; A3FFS8; -.
DR   SMR; A3FFS8; -.
DR   Ensembl; ENSBGRT00000035954; ENSBGRP00000031084; ENSBGRG00000019517.
DR   Ensembl; ENSBGRT00000036028; ENSBGRP00000031151; ENSBGRG00000019517.
DR   GeneTree; ENSGT00390000017226; -.
DR   Proteomes; UP000694520; Chromosome 26.
DR   GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR   GO; GO:0005615; C:extracellular space; IEA:Ensembl.
DR   GO; GO:0005125; F:cytokine activity; IEA:Ensembl.
DR   GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0030295; F:protein kinase activator activity; IEA:Ensembl.
DR   GO; GO:0071474; P:cellular hyperosmotic response; IEA:Ensembl.
DR   GO; GO:0007566; P:embryo implantation; IEA:Ensembl.
DR   GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
DR   GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR   GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR   GO; GO:0042541; P:hemoglobin biosynthetic process; IEA:Ensembl.
DR   GO; GO:0033028; P:myeloid cell apoptotic process; IEA:Ensembl.
DR   GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; IEA:Ensembl.
DR   GO; GO:2001258; P:negative regulation of cation channel activity; IEA:Ensembl.
DR   GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; IEA:Ensembl.
DR   GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0018105; P:peptidyl-serine phosphorylation; IEA:Ensembl.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR   GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IEA:Ensembl.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:Ensembl.
DR   GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IEA:Ensembl.
DR   GO; GO:0001666; P:response to hypoxia; IEA:Ensembl.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR019767; EPO/TPO_CS.
DR   InterPro; IPR001323; EPO_TPO.
DR   InterPro; IPR003013; Erythroptn.
DR   PANTHER; PTHR10370; PTHR10370; 1.
DR   Pfam; PF00758; EPO_TPO; 1.
DR   PIRSF; PIRSF001951; EPO; 1.
DR   PRINTS; PR00272; ERYTHROPTN.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00817; EPO_TPO; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..192
FT                   /note="Erythropoietin"
FT                   /id="PRO_0000313662"
FT   CARBOHYD        49
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        63
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        108
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        32..187
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..58
FT                   /evidence="ECO:0000250"
FT   VARIANT         86
FT                   /note="Q -> L (in strain: JiuLong)"
FT                   /evidence="ECO:0000269|Ref.1"
SQ   SEQUENCE   192 AA;  21090 MW;  198D32938DF16098 CRC64;
     MGARDCTPLL MLSFLLFPLG FPVLGAPARL ICDSRVLERY ILEAREAENA TMGCAEGCSF
     NENITVPDTK VNFYAWKRME VQQQAQEVWQ GLALLSEAIL RGQALLANAS QPCEALRLHV
     DKAVSGLRSL TSLLRALGAQ KEAISLPDAT PSAAPLRAFT VDALSKLFRI YSNFLRGKLT
     LYTGEACRRG DR