ID   EPO_CANLF               Reviewed;         206 AA.
AC   P33707; Q6PWU5;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 2.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Erythropoietin;
DE   Flags: Precursor;
GN   Name=EPO;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Kidney;
RA   Souza D.S., Vicentim D.L., Costa F.F., Saad S.T.O.;
RT   "Description of the full length of canine erythropoietin.";
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 19-193.
RX   PubMed=8364201;
RA   Wen D., Boissel J.-P.R., Tracy T.E., Gruninger R.H., Mulcahy L.S.,
RA   Czelusniak J., Goodman M., Bunn H.F.;
RT   "Erythropoietin structure-function relationships: high degree of sequence
RT   homology among mammals.";
RL   Blood 82:1507-1516(1993).
CC   -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC       proliferation and differentiation and the maintenance of a
CC       physiological level of circulating erythrocyte mass. Binds to EPOR
CC       leading to EPOR dimerization and JAK2 activation thereby activating
CC       specific downstream effectors, including STAT1 and STAT3.
CC       {ECO:0000250|UniProtKB:P01588}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC       liver of fetal or neonatal mammals.
CC   -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR   EMBL; AY572971; AAS77874.1; -; mRNA.
DR   EMBL; L13027; AAA30842.1; -; mRNA.
DR   PIR; I46199; I46199.
DR   RefSeq; NP_001006647.1; NM_001006646.1.
DR   AlphaFoldDB; P33707; -.
DR   SMR; P33707; -.
DR   STRING; 9615.ENSCAFP00000020937; -.
DR   PaxDb; P33707; -.
DR   GeneID; 404002; -.
DR   CTD; 2056; -.
DR   eggNOG; ENOG502RXRC; Eukaryota.
DR   InParanoid; P33707; -.
DR   OrthoDB; 1175751at2759; -.
DR   Proteomes; UP000002254; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR   GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR   GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
DR   GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR   GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central.
DR   GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IBA:GO_Central.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR019767; EPO/TPO_CS.
DR   InterPro; IPR001323; EPO_TPO.
DR   InterPro; IPR003013; Erythroptn.
DR   PANTHER; PTHR10370; PTHR10370; 1.
DR   Pfam; PF00758; EPO_TPO; 1.
DR   PIRSF; PIRSF001951; EPO; 1.
DR   PRINTS; PR00272; ERYTHROPTN.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00817; EPO_TPO; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..40
FT                   /evidence="ECO:0000250"
FT   CHAIN           41..206
FT                   /note="Erythropoietin"
FT                   /id="PRO_0000008398"
FT   CARBOHYD        64
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        78
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        123
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        47..201
FT                   /evidence="ECO:0000250"
FT   DISULFID        69..73
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   206 AA;  22666 MW;  1EEC64A02CE4F5B0 CRC64;
     MCEPAPPKPT QSAWHSFPEC PALLLLLSLL LLPLGLPVLG APPRLICDSR VLERYILEAR
     EAENVTMGCA QGCSFSENIT VPDTKVNFYT WKRMDVGQQA LEVWQGLALL SEAILRGQAL
     LANASQPSET PQLHVDKAVS SLRSLTSLLR ALGAQKEAMS LPEEASPAPL RTFTVDTLCK
     LFRIYSNFLR GKLTLYTGEA CRRGDR