ID   EPO_DANRE               Reviewed;         183 AA.
AC   Q2XNF5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Erythropoietin;
DE   AltName: Full=Erythropoietin-L2;
DE   Flags: Precursor;
GN   Name=epo;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Paffett-Lugassy N., Yang C., Paw B., Leschinsky I., Barut B., Bahary N.,
RA   Caro J., Handin R., Zon L.;
RT   "Functional conservation of epo-epor signaling in zebrafish.";
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR
RP   LOCATION, AND GLYCOSYLATION.
RX   PubMed=17706649; DOI=10.1016/j.febslet.2007.07.073;
RA   Chu C.-Y., Cheng C.-H., Chen G.-D., Chen Y.-C., Hung C.-C., Huang K.-Y.,
RA   Huang C.-J.;
RT   "The zebrafish erythropoietin: functional identification and biochemical
RT   characterization.";
RL   FEBS Lett. 581:4265-4271(2007).
CC   -!- FUNCTION: Erythropoietin is the principal hormone involved in the
CC       regulation of erythrocyte differentiation and the maintenance of a
CC       physiological level of circulating erythrocyte mass.
CC       {ECO:0000269|PubMed:17706649}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17706649}.
CC   -!- TISSUE SPECIFICITY: Expressed in heart and liver.
CC       {ECO:0000269|PubMed:17706649}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:17706649}.
CC   -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR   EMBL; DQ278896; ABB77436.1; -; mRNA.
DR   EMBL; EF426727; ABQ41211.1; -; mRNA.
DR   RefSeq; NP_001033098.1; NM_001038009.2.
DR   AlphaFoldDB; Q2XNF5; -.
DR   SMR; Q2XNF5; -.
DR   STRING; 7955.ENSDARP00000100433; -.
DR   PaxDb; Q2XNF5; -.
DR   Ensembl; ENSDART00000111066; ENSDARP00000100433; ENSDARG00000055163.
DR   GeneID; 100004455; -.
DR   KEGG; dre:100004455; -.
DR   CTD; 100004455; -.
DR   ZFIN; ZDB-GENE-061218-3; epoa.
DR   eggNOG; ENOG502RXRC; Eukaryota.
DR   GeneTree; ENSGT00390000017226; -.
DR   InParanoid; Q2XNF5; -.
DR   TreeFam; TF333413; -.
DR   Reactome; R-DRE-9027276; Erythropoietin activates Phosphoinositide-3-kinase (PI3K).
DR   PRO; PR:Q2XNF5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 7.
DR   Bgee; ENSDARG00000055163; Expressed in cardiac ventricle and 27 other tissues.
DR   ExpressionAtlas; Q2XNF5; baseline.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:ZFIN.
DR   GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR   GO; GO:0005128; F:erythropoietin receptor binding; IEA:InterPro.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR   GO; GO:0030097; P:hemopoiesis; IDA:ZFIN.
DR   GO; GO:0048823; P:nucleate erythrocyte development; IDA:ZFIN.
DR   GO; GO:0048793; P:pronephros development; IMP:ZFIN.
DR   GO; GO:0014823; P:response to activity; IDA:ZFIN.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR001323; EPO_TPO.
DR   InterPro; IPR003013; Erythroptn.
DR   PANTHER; PTHR10370; PTHR10370; 1.
DR   Pfam; PF00758; EPO_TPO; 1.
DR   PIRSF; PIRSF001951; EPO; 1.
DR   PRINTS; PR00272; ERYTHROPTN.
DR   SUPFAM; SSF47266; SSF47266; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..183
FT                   /note="Erythropoietin"
FT                   /id="PRO_0000313665"
FT   CARBOHYD        61
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        104
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        30..178
FT                   /evidence="ECO:0000250"
FT   DISULFID        52..56
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   183 AA;  20384 MW;  AF8E73FF524DBD50 CRC64;
     MFHGSGLFAL LLMVLEWTRP GLSSPLRPIC DLRVLDHFIK EAWDAEAAMR TCKDDCSIAT
     NVTVPLTRVD FEVWEAMNIE EQAQEVQSGL HMLNEAIGSL QISNQTEVLQ SHIDASIRNI
     ASIRQVLRSL SIPEYVPPTS SGEDKETQKI SSISELFQVH VNFLRGKARL LLANAPVCRQ
     GVS