EPO_MOUSE
ID EPO_MOUSE Reviewed; 192 AA.
AC P07321;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Erythropoietin;
DE Flags: Precursor;
GN Name=Epo;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3773894; DOI=10.1128/mcb.6.3.849-858.1986;
RA Shoemaker C.B., Mitsock L.D.;
RT "Murine erythropoietin gene: cloning, expression, and human gene
RT homology.";
RL Mol. Cell. Biol. 6:849-858(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3022133; DOI=10.1128/mcb.6.3.842-848.1986;
RA McDonald J.D., Lin F.-K., Goldwasser E.;
RT "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin
RT gene.";
RL Mol. Cell. Biol. 6:842-848(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=11239002; DOI=10.1093/nar/29.6.1352;
RA Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P.,
RA Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W.,
RA Koop B.F.;
RT "Comparative analysis of the gene-dense ACHE/TFR2 region on human
RT chromosome 7q22 with the orthologous region on mouse chromosome 5.";
RL Nucleic Acids Res. 29:1352-1365(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-52.
RC STRAIN=ICFW;
RX PubMed=9365246; DOI=10.1038/sj.onc.1201364;
RA Chretien S., Duprez V., Maouche L., Gisselbrecht S., Mayeux P., Lacombe C.;
RT "Abnormal erythropoietin (Epo) gene expression in the murine
RT erythroleukemia IW32 cells results from a rearrangement between the G-
RT protein beta2 subunit gene and the Epo gene.";
RL Oncogene 15:1995-1999(1997).
CC -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC proliferation and differentiation and the maintenance of a
CC physiological level of circulating erythrocyte mass. Binds to EPOR
CC leading to EPOR dimerization and JAK2 activation thereby activating
CC specific downstream effectors, including STAT1 and STAT3.
CC {ECO:0000250|UniProtKB:P01588}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC liver of fetal or neonatal mammals.
CC -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR EMBL; M12482; AAA37568.1; -; Genomic_DNA.
DR EMBL; M12930; AAA37570.1; -; Genomic_DNA.
DR EMBL; AF312033; AAK28825.1; -; Genomic_DNA.
DR EMBL; Y11971; CAA72707.1; -; mRNA.
DR CCDS; CCDS39332.1; -.
DR PIR; A24902; A24902.
DR RefSeq; NP_031968.1; NM_007942.2.
DR AlphaFoldDB; P07321; -.
DR SMR; P07321; -.
DR STRING; 10090.ENSMUSP00000031723; -.
DR GlyGen; P07321; 3 sites.
DR PhosphoSitePlus; P07321; -.
DR PaxDb; P07321; -.
DR PRIDE; P07321; -.
DR Antibodypedia; 4151; 1289 antibodies from 40 providers.
DR DNASU; 13856; -.
DR Ensembl; ENSMUST00000031723; ENSMUSP00000031723; ENSMUSG00000029711.
DR Ensembl; ENSMUST00000111038; ENSMUSP00000106667; ENSMUSG00000029711.
DR GeneID; 13856; -.
DR KEGG; mmu:13856; -.
DR UCSC; uc009acm.1; mouse.
DR CTD; 2056; -.
DR MGI; MGI:95407; Epo.
DR VEuPathDB; HostDB:ENSMUSG00000029711; -.
DR eggNOG; ENOG502RXRC; Eukaryota.
DR GeneTree; ENSGT00390000017226; -.
DR InParanoid; P07321; -.
DR OMA; GVRECPA; -.
DR OrthoDB; 1175751at2759; -.
DR PhylomeDB; P07321; -.
DR TreeFam; TF333413; -.
DR Reactome; R-MMU-9027276; Erythropoietin activates Phosphoinositide-3-kinase (PI3K).
DR Reactome; R-MMU-9027284; Erythropoietin activates RAS.
DR SABIO-RK; P07321; -.
DR BioGRID-ORCS; 13856; 1 hit in 75 CRISPR screens.
DR PRO; PR:P07321; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P07321; protein.
DR Bgee; ENSMUSG00000029711; Expressed in vasculature of trunk and 31 other tissues.
DR ExpressionAtlas; P07321; baseline and differential.
DR GO; GO:0044297; C:cell body; ISO:MGI.
DR GO; GO:0009986; C:cell surface; ISO:MGI.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0005125; F:cytokine activity; ISO:MGI.
DR GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; IDA:UniProtKB.
DR GO; GO:0006953; P:acute-phase response; IEA:Ensembl.
DR GO; GO:0007568; P:aging; IEA:Ensembl.
DR GO; GO:0001525; P:angiogenesis; TAS:DFLAT.
DR GO; GO:0006915; P:apoptotic process; IDA:MGI.
DR GO; GO:0055008; P:cardiac muscle tissue morphogenesis; TAS:DFLAT.
DR GO; GO:0071474; P:cellular hyperosmotic response; ISO:MGI.
DR GO; GO:0007566; P:embryo implantation; IDA:MGI.
DR GO; GO:0030218; P:erythrocyte differentiation; IDA:MGI.
DR GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0003007; P:heart morphogenesis; TAS:DFLAT.
DR GO; GO:0042541; P:hemoglobin biosynthetic process; IDA:MGI.
DR GO; GO:0033028; P:myeloid cell apoptotic process; IDA:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
DR GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; ISO:MGI.
DR GO; GO:2001258; P:negative regulation of cation channel activity; ISO:MGI.
DR GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; ISO:MGI.
DR GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; ISO:MGI.
DR GO; GO:0033033; P:negative regulation of myeloid cell apoptotic process; IDA:MGI.
DR GO; GO:1901215; P:negative regulation of neuron death; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IDA:UniProtKB.
DR GO; GO:0042104; P:positive regulation of activated T cell proliferation; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:MGI.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR GO; GO:0045860; P:positive regulation of protein kinase activity; IDA:UniProtKB.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0048678; P:response to axon injury; IEA:Ensembl.
DR GO; GO:0071548; P:response to dexamethasone; IEA:Ensembl.
DR GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl.
DR GO; GO:0043627; P:response to estrogen; IEA:Ensembl.
DR GO; GO:0055093; P:response to hyperoxia; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IDA:MGI.
DR GO; GO:0070555; P:response to interleukin-1; IEA:Ensembl.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0009651; P:response to salt stress; IEA:Ensembl.
DR GO; GO:0033574; P:response to testosterone; IEA:Ensembl.
DR GO; GO:0033189; P:response to vitamin A; IEA:Ensembl.
DR GO; GO:0060979; P:vasculogenesis involved in coronary vascular morphogenesis; TAS:DFLAT.
DR GO; GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; TAS:DFLAT.
DR GO; GO:0061032; P:visceral serous pericardium development; TAS:DFLAT.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR019767; EPO/TPO_CS.
DR InterPro; IPR001323; EPO_TPO.
DR InterPro; IPR003013; Erythroptn.
DR PANTHER; PTHR10370; PTHR10370; 1.
DR Pfam; PF00758; EPO_TPO; 1.
DR PIRSF; PIRSF001951; EPO; 1.
DR PRINTS; PR00272; ERYTHROPTN.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00817; EPO_TPO; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT CHAIN 27..192
FT /note="Erythropoietin"
FT /id="PRO_0000008405"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT DISULFID 33..187
FT /evidence="ECO:0000250"
SQ SEQUENCE 192 AA; 21365 MW; 65F94E214E0DEF2E CRC64;
MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR
LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI LQAQALLANS SQPPETLQLH
IDKAISGLRS LTSLLRVLGA QKELMSPPDT TPPAPLRTLT VDTFCKLFRV YANFLRGKLK
LYTGEVCRRG DR
