EPO_PIG
ID EPO_PIG Reviewed; 194 AA.
AC P49157; Q9MYM8;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Erythropoietin;
DE Flags: Precursor;
GN Name=EPO;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=Norwegian Landrace; TISSUE=Kidney;
RX PubMed=11311851; DOI=10.1016/s0739-7240(01)00089-3;
RA David R.B., Blom A.K., Sjaastad O.V., Harbitz I.;
RT "The porcine erythropoietin gene: cDNA sequence, genomic sequence and
RT expression analyses in piglets.";
RL Domest. Anim. Endocrinol. 20:137-147(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 5-194.
RC TISSUE=Kidney;
RX PubMed=8364201;
RA Wen D., Boissel J.-P.R., Tracy T.E., Gruninger R.H., Mulcahy L.S.,
RA Czelusniak J., Goodman M., Bunn H.F.;
RT "Erythropoietin structure-function relationships: high degree of sequence
RT homology among mammals.";
RL Blood 82:1507-1516(1993).
CC -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC proliferation and differentiation and the maintenance of a
CC physiological level of circulating erythrocyte mass. Binds to EPOR
CC leading to EPOR dimerization and JAK2 activation thereby activating
CC specific downstream effectors, including STAT1 and STAT3.
CC {ECO:0000250|UniProtKB:P01588}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC liver of fetal or neonatal mammals.
CC -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR EMBL; AJ249745; CAB96416.1; -; mRNA.
DR EMBL; AJ249746; CAB96417.1; -; Genomic_DNA.
DR EMBL; L10607; AAA31029.1; -; mRNA.
DR PIR; I46578; I46578.
DR RefSeq; NP_999299.1; NM_214134.1.
DR AlphaFoldDB; P49157; -.
DR SMR; P49157; -.
DR STRING; 9823.ENSSSCP00000008194; -.
DR PaxDb; P49157; -.
DR PRIDE; P49157; -.
DR Ensembl; ENSSSCT00000008413; ENSSSCP00000008194; ENSSSCG00000007673.
DR Ensembl; ENSSSCT00005000040; ENSSSCP00005000009; ENSSSCG00005000027.
DR Ensembl; ENSSSCT00025100933; ENSSSCP00025044599; ENSSSCG00025073331.
DR Ensembl; ENSSSCT00030013330; ENSSSCP00030005994; ENSSSCG00030009733.
DR Ensembl; ENSSSCT00035097698; ENSSSCP00035041231; ENSSSCG00035072206.
DR Ensembl; ENSSSCT00040103619; ENSSSCP00040047022; ENSSSCG00040074856.
DR Ensembl; ENSSSCT00045031528; ENSSSCP00045021842; ENSSSCG00045018517.
DR Ensembl; ENSSSCT00055050476; ENSSSCP00055040356; ENSSSCG00055025537.
DR Ensembl; ENSSSCT00065019511; ENSSSCP00065007977; ENSSSCG00065014655.
DR Ensembl; ENSSSCT00070032133; ENSSSCP00070026806; ENSSSCG00070016325.
DR GeneID; 397249; -.
DR KEGG; ssc:397249; -.
DR CTD; 2056; -.
DR VGNC; VGNC:87744; EPO.
DR eggNOG; ENOG502RXRC; Eukaryota.
DR GeneTree; ENSGT00390000017226; -.
DR HOGENOM; CLU_110946_0_0_1; -.
DR InParanoid; P49157; -.
DR OMA; GVRECPA; -.
DR OrthoDB; 1175751at2759; -.
DR TreeFam; TF333413; -.
DR Reactome; R-SSC-9027276; Erythropoietin activates Phosphoinositide-3-kinase (PI3K).
DR Reactome; R-SSC-9027284; Erythropoietin activates RAS.
DR Proteomes; UP000008227; Chromosome 3.
DR Proteomes; UP000314985; Chromosome 3.
DR Bgee; ENSSSCG00000007673; Expressed in limb bud and 1 other tissue.
DR Genevisible; P49157; SS.
DR GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR GO; GO:0071474; P:cellular hyperosmotic response; IEA:Ensembl.
DR GO; GO:0007566; P:embryo implantation; IEA:Ensembl.
DR GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
DR GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0042541; P:hemoglobin biosynthetic process; IEA:Ensembl.
DR GO; GO:0033028; P:myeloid cell apoptotic process; IEA:Ensembl.
DR GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; IEA:Ensembl.
DR GO; GO:2001258; P:negative regulation of cation channel activity; IEA:Ensembl.
DR GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; IEA:Ensembl.
DR GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:Ensembl.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR019767; EPO/TPO_CS.
DR InterPro; IPR001323; EPO_TPO.
DR InterPro; IPR003013; Erythroptn.
DR PANTHER; PTHR10370; PTHR10370; 1.
DR Pfam; PF00758; EPO_TPO; 1.
DR PIRSF; PIRSF001951; EPO; 1.
DR PRINTS; PR00272; ERYTHROPTN.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00817; EPO_TPO; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..194
FT /note="Erythropoietin"
FT /id="PRO_0000008406"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 172
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 33..189
FT /evidence="ECO:0000250"
FT DISULFID 55..59
FT /evidence="ECO:0000250"
SQ SEQUENCE 194 AA; 21304 MW; 77881A6F6F20EA1C CRC64;
MGARECPARL LLLSLLLLPL GLPVLGAPPR LICDSRVLER YILEAKEGEN ATMGCAESCS
FSENITVPDT KVNFYAWKRM EVQQQAMEVW QGLALLSEAI LQGQALLANS SQPSEALQLH
VDKAVSGLRS LTSLLRALGA QKEAIPLPDA SPSSATPLRT FAVDTLCKLF RNYSNFLRGK
LTLYTGEACR RRDR
