EPO_RAT
ID EPO_RAT Reviewed; 192 AA.
AC P29676; P70504;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Erythropoietin;
DE Flags: Precursor;
GN Name=Epo;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Kidney;
RX PubMed=1420369; DOI=10.1016/0167-4781(92)90146-q;
RA Nagao M., Suga H., Okano M., Masuda S., Narita H., Ikura K., Sasaki R.;
RT "Nucleotide sequence of rat erythropoietin.";
RL Biochim. Biophys. Acta 1171:99-102(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-192.
RC STRAIN=Sprague-Dawley; TISSUE=Kidney;
RX PubMed=8364201;
RA Wen D., Boissel J.-P.R., Tracy T.E., Gruninger R.H., Mulcahy L.S.,
RA Czelusniak J., Goodman M., Bunn H.F.;
RT "Erythropoietin structure-function relationships: high degree of sequence
RT homology among mammals.";
RL Blood 82:1507-1516(1993).
CC -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC proliferation and differentiation and the maintenance of a
CC physiological level of circulating erythrocyte mass. Binds to EPOR
CC leading to EPOR dimerization and JAK2 activation thereby activating
CC specific downstream effectors, including STAT1 and STAT3.
CC {ECO:0000250|UniProtKB:P01588}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC liver of fetal or neonatal mammals.
CC -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D10763; BAA01593.1; -; mRNA.
DR EMBL; L10608; AAA41126.1; -; mRNA.
DR PIR; S28148; S28148.
DR RefSeq; NP_058697.1; NM_017001.1.
DR AlphaFoldDB; P29676; -.
DR SMR; P29676; -.
DR STRING; 10116.ENSRNOP00000001914; -.
DR GlyGen; P29676; 3 sites.
DR PaxDb; P29676; -.
DR Ensembl; ENSRNOT00000001914; ENSRNOP00000001914; ENSRNOG00000001412.
DR GeneID; 24335; -.
DR KEGG; rno:24335; -.
DR UCSC; RGD:2559; rat.
DR CTD; 2056; -.
DR RGD; 2559; Epo.
DR eggNOG; ENOG502RXRC; Eukaryota.
DR GeneTree; ENSGT00390000017226; -.
DR HOGENOM; CLU_110946_0_0_1; -.
DR InParanoid; P29676; -.
DR OMA; GVRECPA; -.
DR OrthoDB; 1175751at2759; -.
DR PhylomeDB; P29676; -.
DR TreeFam; TF333413; -.
DR Reactome; R-RNO-9027276; Erythropoietin activates Phosphoinositide-3-kinase (PI3K).
DR Reactome; R-RNO-9027284; Erythropoietin activates RAS.
DR PRO; PR:P29676; -.
DR Proteomes; UP000002494; Chromosome 12.
DR Bgee; ENSRNOG00000001412; Expressed in ovary and 3 other tissues.
DR Genevisible; P29676; RN.
DR GO; GO:0044297; C:cell body; IDA:RGD.
DR GO; GO:0009986; C:cell surface; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0005125; F:cytokine activity; ISO:RGD.
DR GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; ISO:RGD.
DR GO; GO:0006953; P:acute-phase response; IEP:RGD.
DR GO; GO:0007568; P:aging; IEP:RGD.
DR GO; GO:0006915; P:apoptotic process; ISO:RGD.
DR GO; GO:0071474; P:cellular hyperosmotic response; ISO:RGD.
DR GO; GO:0007566; P:embryo implantation; ISO:RGD.
DR GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
DR GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0042541; P:hemoglobin biosynthetic process; ISO:RGD.
DR GO; GO:0033028; P:myeloid cell apoptotic process; IEA:Ensembl.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; ISO:RGD.
DR GO; GO:2001258; P:negative regulation of cation channel activity; ISO:RGD.
DR GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; ISO:RGD.
DR GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; ISO:RGD.
DR GO; GO:0033033; P:negative regulation of myeloid cell apoptotic process; ISO:RGD.
DR GO; GO:1901215; P:negative regulation of neuron death; IDA:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; ISO:RGD.
DR GO; GO:0042104; P:positive regulation of activated T cell proliferation; IDA:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IMP:RGD.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:RGD.
DR GO; GO:0010976; P:positive regulation of neuron projection development; IDA:RGD.
DR GO; GO:0045860; P:positive regulation of protein kinase activity; ISO:RGD.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0048678; P:response to axon injury; IEP:RGD.
DR GO; GO:0071548; P:response to dexamethasone; IEP:RGD.
DR GO; GO:0051602; P:response to electrical stimulus; IEP:RGD.
DR GO; GO:0043627; P:response to estrogen; IEP:RGD.
DR GO; GO:0055093; P:response to hyperoxia; IEP:RGD.
DR GO; GO:0001666; P:response to hypoxia; IEP:RGD.
DR GO; GO:0070555; P:response to interleukin-1; IEP:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0007584; P:response to nutrient; IEP:RGD.
DR GO; GO:0009651; P:response to salt stress; IEP:RGD.
DR GO; GO:0033574; P:response to testosterone; IEP:RGD.
DR GO; GO:0033189; P:response to vitamin A; IEP:RGD.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR019767; EPO/TPO_CS.
DR InterPro; IPR001323; EPO_TPO.
DR InterPro; IPR003013; Erythroptn.
DR PANTHER; PTHR10370; PTHR10370; 1.
DR Pfam; PF00758; EPO_TPO; 1.
DR PIRSF; PIRSF001951; EPO; 1.
DR PRINTS; PR00272; ERYTHROPTN.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00817; EPO_TPO; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000250"
FT CHAIN 27..192
FT /note="Erythropoietin"
FT /id="PRO_0000008408"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT DISULFID 33..187
FT /evidence="ECO:0000250"
SQ SEQUENCE 192 AA; 21286 MW; 3EA632737E7D2443 CRC64;
MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR
LSENITVPDT KVNFYAWKRM KVEEQAVEVW QGLSLLSEAI LQAQALQANS SQPPESLQLH
IDKAISGLRS LTSLLRVLGA QKELMSPPDA TQAAPLRTLT ADTFCKLFRV YSNFLRGKLK
LYTGEACRRG DR
