ID   EPO_TAKRU               Reviewed;         185 AA.
AC   Q6JV22; Q6JV23;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Erythropoietin;
DE   Flags: Precursor;
GN   Name=epo;
OS   Takifugu rubripes (Japanese pufferfish) (Fugu rubripes).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Takifugu.
OX   NCBI_TaxID=31033;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND ALTERNATIVE
RP   SPLICING.
RX   PubMed=15142879; DOI=10.1182/blood-2003-10-3404;
RA   Chou C.-F., Tohari S., Brenner S., Venkatesh B.;
RT   "Erythropoietin gene from a teleost fish, Fugu rubripes.";
RL   Blood 104:1498-1503(2004).
CC   -!- FUNCTION: Erythropoietin is the principal hormone involved in the
CC       regulation of erythrocyte differentiation and the maintenance of a
CC       physiological level of circulating erythrocyte mass. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6JV22-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6JV22-2; Sequence=VSP_030095;
CC   -!- TISSUE SPECIFICITY: Expressed mainly in heart, liver and brain. Isoform
CC       2 is brain specific. {ECO:0000269|PubMed:15142879}.
CC   -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR   EMBL; AY303753; AAQ72466.1; -; Genomic_DNA.
DR   EMBL; AY303753; AAQ72467.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6JV22; -.
DR   SMR; Q6JV22; -.
DR   Ensembl; ENSTRUT00000068811; ENSTRUP00000069601; ENSTRUG00000029744. [Q6JV22-1]
DR   GeneTree; ENSGT00390000017226; -.
DR   HOGENOM; CLU_2660853_0_0_1; -.
DR   InParanoid; Q6JV22; -.
DR   Proteomes; UP000005226; Chromosome 8.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:Ensembl.
DR   GO; GO:0005128; F:erythropoietin receptor binding; IEA:InterPro.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR   GO; GO:0048823; P:nucleate erythrocyte development; IEA:Ensembl.
DR   GO; GO:0048793; P:pronephros development; IEA:Ensembl.
DR   GO; GO:0014823; P:response to activity; IEA:Ensembl.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR001323; EPO_TPO.
DR   InterPro; IPR003013; Erythroptn.
DR   PANTHER; PTHR10370; PTHR10370; 1.
DR   Pfam; PF00758; EPO_TPO; 1.
DR   PIRSF; PIRSF001951; EPO; 1.
DR   PRINTS; PR00272; ERYTHROPTN.
DR   SUPFAM; SSF47266; SSF47266; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Disulfide bond; Erythrocyte maturation; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..185
FT                   /note="Erythropoietin"
FT                   /id="PRO_0000313667"
FT   DISULFID        32..180
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..58
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..8
FT                   /note="MLQKTGRG -> MEFPR (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_030095"
SQ   SEQUENCE   185 AA;  20549 MW;  9D9C4311F9D2EF4B CRC64;
     MLQKTGRGLL AFLLIVLEWT QPSLPSPLRP ICDLRVLNHF IKEAQDAEAA MKLCSEGCTL
     SDSVIVPQTT VEFDVWEKKS ALAKAQEVQS GLWLLQEAFN FLRTSVTNTA LHSHIDNSVR
     NLLSVNAVLR SLNIQEFTPP ASAAEIEGTW RVSTATELLQ VHINFLRGKV RLILLDAQAC
     QQDVS