ID   EPSD_RALSL              Reviewed;         423 AA.
AC   Q45410;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase;
DE            EC=1.1.1.-;
GN   Name=epsD;
OS   Ralstonia solanacearum (Pseudomonas solanacearum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AW;
RX   PubMed=7476194; DOI=10.1111/j.1365-2958.1995.tb02323.x;
RA   Huang J., Schell M.;
RT   "Molecular characterization of the eps gene cluster of Pseudomonas
RT   solanacearum and its transcriptional regulation at a single promoter.";
RL   Mol. Microbiol. 16:977-989(1995).
CC   -!- FUNCTION: Probably involved in synthesis of sugar components of EPS I,
CC       by converting NDP-N-acetyl-D-galactosamine into NDP-N-acetyl-D-
CC       galactosaminuronic acid.
CC   -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; U17898; AAA91627.1; -; Genomic_DNA.
DR   PIR; S77638; S77638.
DR   AlphaFoldDB; Q45410; -.
DR   SMR; Q45410; -.
DR   STRING; 859657.RPSI07_mp1010; -.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR017476; UDP-Glc/GDP-Man.
DR   InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR   InterPro; IPR036220; UDP-Glc/GDP-Man_DH_C_sf.
DR   InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR   InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR   InterPro; IPR028359; UDP_ManNAc/GlcNAc_DH.
DR   Pfam; PF00984; UDPG_MGDP_dh; 1.
DR   Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR   Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR   PIRSF; PIRSF500136; UDP_ManNAc_DH; 1.
DR   PIRSF; PIRSF000124; UDPglc_GDPman_dh; 1.
DR   SMART; SM00984; UDPG_MGDP_dh_C; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52413; SSF52413; 1.
DR   TIGRFAMs; TIGR03026; NDP-sugDHase; 1.
PE   3: Inferred from homology;
KW   Lipopolysaccharide biosynthesis; NAD; Oxidoreductase.
FT   CHAIN           1..423
FT                   /note="NDP-N-acetyl-D-galactosaminuronic acid
FT                   dehydrogenase"
FT                   /id="PRO_0000074074"
FT   ACT_SITE        218
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:O59284"
FT   ACT_SITE        272
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O59284"
FT   BINDING         11..28
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   423 AA;  46803 MW;  405E93D11A09B13A CRC64;
     MDRAIEIDFR TISVVGLGYI GLPTATVLAS RQRELIGVDI NQHAVDTINQ ARIHIVEPDL
     DMLVRAAVSQ GYLRATTEPE PADAFLIAVP TPFLEDKQPD LTYIEAAAKA IAPVLKRGDL
     VVLESTSPVG ATEQLSAWLS EQRSDLSFPH QLGEESDIRV AHCPERVLPG HVLRELVEND
     RIIGGMTPRC SQAAQRLYEL FVRGRCIVTD ARTAEMCKLT ENAFRDVNIA FANELSMICD
     EIGVNVWELI SVANRHPRVN ILQPGPGVGG HCIAVDPWFI VDAAPESARL IRTAREVNDA
     KPHYVLDRVK QAARRFKEPV IACFGLSFKA NIDDLRESPA IEIVRTMVQQ QLGTVLVVEP
     HIKVLPASLE GVELLNAEPA LSRADIVVLL VDHQKFRKLD TDRLQSRVVI DTRGMWSAKR
     LAA