ID   EPSD_RALSO              Reviewed;         423 AA.
AC   P58591;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase;
DE            EC=1.1.1.-;
GN   Name=epsD; OrderedLocusNames=RSp1016; ORFNames=RS02350;
OS   Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OG   Plasmid megaplasmid Rsp.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- FUNCTION: Probably involved in the synthesis of sugar components of EPS
CC       I, by converting NDP-N-acetyl-D-galactosamine into NDP-N-acetyl-D-
CC       galactosaminuronic acid. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; AL646053; CAD18167.1; -; Genomic_DNA.
DR   RefSeq; WP_011004306.1; NC_003296.1.
DR   AlphaFoldDB; P58591; -.
DR   SMR; P58591; -.
DR   STRING; 267608.RSp1016; -.
DR   EnsemblBacteria; CAD18167; CAD18167; RSp1016.
DR   GeneID; 60503927; -.
DR   KEGG; rso:RSp1016; -.
DR   eggNOG; COG0677; Bacteria.
DR   HOGENOM; CLU_023810_3_2_4; -.
DR   OMA; IELANTH; -.
DR   Proteomes; UP000001436; Plasmid megaplasmid Rsp.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR017476; UDP-Glc/GDP-Man.
DR   InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR   InterPro; IPR036220; UDP-Glc/GDP-Man_DH_C_sf.
DR   InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR   InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR   InterPro; IPR028359; UDP_ManNAc/GlcNAc_DH.
DR   Pfam; PF00984; UDPG_MGDP_dh; 1.
DR   Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR   Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR   PIRSF; PIRSF500136; UDP_ManNAc_DH; 1.
DR   PIRSF; PIRSF000124; UDPglc_GDPman_dh; 1.
DR   SMART; SM00984; UDPG_MGDP_dh_C; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52413; SSF52413; 1.
DR   TIGRFAMs; TIGR03026; NDP-sugDHase; 1.
PE   3: Inferred from homology;
KW   Lipopolysaccharide biosynthesis; NAD; Oxidoreductase; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..423
FT                   /note="NDP-N-acetyl-D-galactosaminuronic acid
FT                   dehydrogenase"
FT                   /id="PRO_0000074073"
FT   ACT_SITE        218
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:O59284"
FT   ACT_SITE        272
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O59284"
FT   BINDING         11..28
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   423 AA;  46673 MW;  14A719FF9D822FDB CRC64;
     MDRAIDIDFR TISVVGLGYI GLPTATVLAS RQREVIGVDI NQHAVDTINQ GRIHIVEPDL
     DMLVRAAVSQ GYLRATTEPE PADAFLIAVP TPFLDNKQPD LSYIEAAARA IAPVLKRGDL
     VVLESTSPVG ATEQLSDWLS AQRPDLSFPH QQGEESDIRV AHCPERVLPG HVLRELVEND
     RIIGGMTPKC SEAAQRLYEL FVRGRCIVTD ARTAEMCKLT ENAFRDVNIA FANELSMICD
     EIGVNVWELI SVANRHPRVN ILQPGPGVGG HCIAVDPWFI VDAAPESARL IRTAREVNDA
     KPHYVLDRVK QAARRFKEPV IACFGLSFKA NIDDLRESPA IEIVQTMVQQ QLGTVLVVEP
     HIKVLPAALQ GVELLNAEAA LSRADIVVLL VDHQQFRKLD TDRLQSRVVI DTRGMWSAKR
     IAA